Invitrogen

Fibronectin Monoclonal Antibody (FN-3), eBioscience™

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

9 Published Figures

6 References

View all (101) Fibronectin antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Fibronectin Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 15

Fibronectin Antibody (14-9869-82) in ICC/IF

Immunofluorescence analysis of Fibronectin was performed using 70% confluent log phase Hep G2 cells treated with 1X PTI for 4h. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Fibronectin Monoclonal Antibody (FN-3), eBioscience™ (Product # 14-9869-82) at... View More

Fibronectin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Fibronectin Antibody in Western Blot (WB)

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Product Details

14-9869-82

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5 µg/mL

Immunohistochemistry (Paraffin) (IHC (P))

10 µg/mL

Immunocytochemistry (ICC/IF)

0.025 µg/mL

View 6 publications 6 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

FN-3

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11040270

Product Specific Information

Description: The FN-3 monoclonal antibody recognizes human fibronectin, a component of the extracellular matrix. This high molecular weight protein (almost 400 kDa) exists as an extracellular and a soluble plasma form. Fibronectin functions in cell adhesion and migration via integrins present on the interacting cell. Fibronectin also associates with collagen, actin and fibrins. In malignancies, fibronectin protein levels can be altered. In lung carninomas, expression is decreased. The FN-3 antibody recognizes a determinant on human cellular but not plasma fibronectin; this recognition is not lost upon trypsin treatment. Furthermore, FN-3 antibody has been shown to crossreact to bovine fibronectin.

Applications Reported: This FN-3 antibody has been reported for use in immunohistochemical staining of formalin-fixed paraffin embedded (FFPE) tissue sections and immunoblotting.

Applications Tested: This FN-3 antibody has been tested by immunohistochemistry on FFPE human placenta (with IHC Antigen Retrieval Solution - Low pH (Product # 00-4955-58) and can be used at less than or equal to 10 µg/mL. Is is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Fibronectin is a disulfide-bonded dimer with a molecular weight range of 230-250 kDa. In the extracellular matrix of several connective tissues and vessels, fibronectin is present as an insoluble protein that is extensively cross-linked by interchain disulfide bonds forming high molecular mass polymers. Fibronectin is most abundant during embryonic development and tissue remodeling. Fibronectin is also present at high concentrations as a soluble plasma protein. Fibronectin is organized as a linear series of repeating modules which form domains for interaction with fibronectin itself, other matrix components (e.g. collagen and heparin) and receptors on cells (e.g. integrins). Fibronectin is present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. Fibronectin has been implicated in carcinoma development in lung cancer. Further, Fibronectin expression is increased especially in non-small cell lung carcinoma. The adhesion of lung carcinoma cells to Fibronectin enhances tumorgenecity and confers resistance to apoptosis induced by standard chemotherapeutic agents. The gene encoding Fibronectin has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some Fibronectin variants has not been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Increased Stiffness Downregulates Focal Adhesion Kinase Expression in Pancreatic Cancer Cells Cultured in 3D Self-Assembling Peptide Scaffolds.

Biomedicines

Betriu N,Andreeva A,Alonso A,Semino CE

Published figure using Fibronectin monoclonal antibody (Product # 14-9869-82) in Immunocytochemistry

Fri Jul 29 00:00:00 EDT 2022

3D Extracellular Matrix Regulates the Activity of T Cells and Cancer Associated Fibroblasts in Breast Cancer.

Frontiers in oncology

Gao H,Tian Q,Zhu L,Feng J,Zhou Y,Yang J

Published figure using Fibronectin monoclonal antibody (Product # 14-9869-82) in Immunocytochemistry

Fri Apr 29 00:00:00 EDT 2022

Application of a High-Content Screening Assay Utilizing Primary Human Lung Fibroblasts to Identify Antifibrotic Drugs for Rapid Repurposing in COVID-19 Patients.

SLAS discovery : advancing life sciences R & D

Marwick JA,Elliott RJR,Longden J,Makda A,Hirani N,Dhaliwal K,Dawson JC,Carragher NO

Published figure using Fibronectin monoclonal antibody (Product # 14-9869-82) in Immunocytochemistry

Fri Oct 01 00:00:00 EDT 2021

Spontaneous Extracellular Matrix Accumulation in a Human in vitro Model of Renal Fibrosis Is Mediated by αV Integrins.

Nephron

Bon H,Hales P,Lumb S,Holdsworth G,Johnson T,Qureshi O,Twomey BM

14-9869 was used in Immunocytochemistry to test the hypothesis that integrins are pro-fibrotic via regulation of functional interactions between tubular epithelial cells and renal fibroblasts.

Tue Sep 01 00:00:00 EDT 2020

A high content, phenotypic 'scar-in-a-jar' assay for rapid quantification of collagen fibrillogenesis using disease-derived pulmonary fibroblasts.

BMC biomedical engineering

Good RB,Eley JD,Gower E,Butt G,Blanchard AD,Fisher AJ,Nanthakumar CB

Published figure using Fibronectin monoclonal antibody (Product # 14-9869-82) in Immunocytochemistry

Sat Apr 16 00:00:00 EDT 2022

PEGylated pUR4/FUD peptide inhibitor of fibronectin fibrillogenesis decreases fibrosis in murine Unilateral Ureteral Obstruction model of kidney disease.

PloS one

Tomasini-Johansson BR,Zbyszynski PW,Toraason I,Peters DM,Kwon GS

Published figure using Fibronectin monoclonal antibody (Product # 14-9869-82) in Immunofluorescence

Fri Mar 22 00:00:00 EDT 2019

Bioinformatics

Protein Aliases: CIG; Cold insoluble globulin (CIG); Cold-insoluble globulin; DKFZp686F10164; DKFZp686H0342; DKFZp686I1370; DKFZp686O13149; ferritin L subunit; ferritin L-chain; Fibronectin; FN; Migration stimulating factor (MSF); migration-stimulating factor

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Gene Aliases: CIG; ED-B; FINC; FN; FN1; FNZ; GFND; GFND2; LETS; MSF

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UniProt ID: (Human) P02751

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Entrez Gene ID: (Human) 2335

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Function(s)

protease binding integrin binding protein binding collagen binding heparin binding peptidase activator activity identical protein binding mercury ion binding extracellular matrix protein extracellular matrix structural protein

Process(es)

angiogenesis cell activation regulation of protein phosphorylation platelet degranulation response to ischemia acute-phase response cell-substrate junction assembly cell adhesion calcium-independent cell-matrix adhesion positive regulation of cell proliferation glial cell migration regulation of cell shape response to wounding response to ozone positive regulation of gene expression positive regulation of peptidase activity peptide cross-linking extracellular matrix disassembly extracellular matrix organization integrin activation substrate adhesion-dependent cell spreading cellular response to vascular endothelial growth factor stimulus endodermal cell differentiation cellular response to platelet-derived growth factor stimulus wound healing negative regulation of apoptotic process positive regulation of axon extension positive regulation of fibroblast proliferation leukocyte migration positive regulation of chemotaxis response to glucocorticoid regulation of ERK1 and ERK2 cascade cellular response to lipopolysaccharide cellular response to mercury ion cellular response to glucose stimulus cellular response to interleukin-1 cellular response to prostaglandin E stimulus cellular response to transforming growth factor beta stimulus cellular response to BMP stimulus positive regulation of substrate-dependent cell migration, cell attachment to substrate negative regulation of transforming growth factor-beta secretion

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Fibronectin Monoclonal Antibody (FN-3), eBioscience™

Fibronectin Antibody (14-9869-82) in ICC/IF

Product Details

14-9869-82

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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