Invitrogen

HLA-E Monoclonal Antibody (3D12HLA-E), eBioscience™

FIGURE: 1 / 1

HLA-E Antibody (14-9953-82) in Flow

Staining of normal human peripheral blood cells with 0.5 µg of Mouse IgG1 K Isotype Control Purified (Product # 14-4714-82) (open histogram) or 0.5 µg of Anti-Human HLA-E Purified (filled histogram) followed by Anti-Mouse IgG Biotin (Product # 13-4013-85)and Streptavidin PE (Product # 12-4317-87).Cells in the lymphocyte gate were used for analysis.

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Product Details

14-9953-82

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-Dependent

Flow Cytometry (Flow)

1 µg/test

View 4 publications 4 publications

Immunoprecipitation (IP)

Assay-Dependent

Neutralization (Neu)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

3D12HLA-E

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1210774

Product Specific Information

Description: The monoclonal antibody 3D12 (HLA-E) recognizes human HLA-E, a member of the Human Leukocyte Antigen family. HLA-E is a MHC Class IB member, which has a specialized role in NK cell recognition. Binding of a restricted set of peptides derived from other Class I leader sequences to the HLA-E and B2M (beta2 microglobulin) complex leads to interaction with NKG2A on NK cells. This binding protects the HLA-G-expressing cell from NK cell killing. HLA-E expression is ubiquitous.

Applications Tested: This 3D12 (HLA-E) antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

HLA-E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-E binds a restricted subset of peptides derived from the leader peptides of other class I molecules. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail.

HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

HLA-E-expressing pluripotent stem cells escape allogeneic responses and lysis by NK cells.

Nature biotechnology

Gornalusse GG,Hirata RK,Funk SE,Riolobos L,Lopes VS,Manske G,Prunkard D,Colunga AG,Hanafi LA,Clegg DO,Turtle C,Russell DW

Tue Aug 01 00:00:00 EDT 2017

High-efficiency lysis of cervical cancer by allogeneic NK cells derived from umbilical cord progenitors is independent of HLA status.

Cancer immunology, immunotherapy : CII

Veluchamy JP,Heeren AM,Spanholtz J,van Eendenburg JD,Heideman DA,Kenter GG,Verheul HM,van der Vliet HJ,Jordanova ES,de Gruijl TD

14-9953 was used in Flow cytometry/Cell sorting to provide a rationale to initiate a clinical trial for cervical cancer with adoptively transferred allogeneic NK cells, employing either UCB-NK or PBNK + CET for EGFR-expressing tumors.

Sun Jan 01 00:00:00 EST 2017

EBV BILF1 evolved to downregulate cell surface display of a wide range of HLA class I molecules through their cytoplasmic tail.

Journal of immunology (Baltimore, Md. : 1950)

Griffin BD,Gram AM,Mulder A,Van Leeuwen D,Claas FH,Wang F,Ressing ME,Wiertz E

14-9953 was used in Flow cytometry/Cell sorting to show that the cytoplasmic C-terminal tail of EBV BILF1 is required for reducing surface HLA class I expression.

Fri Feb 15 00:00:00 EST 2013

Restoration of TLR3-activated myeloid dendritic cell activity leads to improved natural killer cell function in chronic hepatitis B virus infection.

Journal of virology

Tjwa ET,van Oord GW,Biesta PJ,Boonstra A,Janssen HL,Woltman AM

14-9953 was used in Flow cytometry/Cell sorting to investigate the functional interaction of myeloid dendritic cells and natural killer cells in chronic hepatitis B.

Sun Apr 01 00:00:00 EDT 2012

Selenite induces posttranscriptional blockade of HLA-E expression and sensitizes tumor cells to CD94/NKG2A-positive NK cells.

Journal of immunology (Baltimore, Md. : 1950)

Enqvist M,Nilsonne G,Hammarfjord O,Wallin RP,Björkström NK,Björnstedt M,Hjerpe A,Ljunggren HG,Dobra K,Malmberg KJ,Carlsten M

14-9953 was used in Flow cytometry/Cell sorting to suggest that selenite may be used to potentiate the anti-tumor cytotoxicity in settings of NK cell-based immunotherapies.

Sat Oct 01 00:00:00 EDT 2011

Bioinformatics

Protein Aliases: DAMA-277I14.1; DAQB-90C11.16; DKFZp686P19218; FLJ26655; HLA class I histocompatibility antigen, alpha chain E; HLA class I histocompatibility antigen, E alpha chain; lymphocyte antigen; MHC 1; MHC class I antigen E; MHC HLA-E alpha-1; MHC HLA-E alpha-2.1; MHC1

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Gene Aliases: EA1.2; EA2.1; HLA-6.2; HLA-E; HLAE; MHC; QA1

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UniProt ID: (Human) P13747

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Entrez Gene ID: (Human) 3133

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Function(s)

receptor binding beta-2-microglobulin binding MHC class I protein binding peptide antigen binding natural killer cell lectin-like receptor binding major histocompatibility complex protein

Process(es)

positive regulation of T cell mediated cytotoxicity adaptive immune response antigen processing and presentation of peptide antigen via MHC class I antigen processing and presentation of endogenous peptide antigen via MHC class Ib antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent regulation of natural killer cell mediated immunity positive regulation of natural killer cell mediated immunity antibacterial humoral response positive regulation of interleukin-13 production positive regulation of interleukin-4 production positive regulation of TRAIL production positive regulation of tumor necrosis factor production CD8-positive, alpha-beta T cell activation protection from natural killer cell mediated cytotoxicity innate immune response regulation of immune response defense response to Gram-positive bacterium positive regulation of immunoglobulin secretion interferon-gamma-mediated signaling pathway type I interferon signaling pathway positive regulation of CD8-positive, alpha-beta T cell proliferation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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HLA-E Monoclonal Antibody (3D12HLA-E), eBioscience™

HLA-E Antibody (14-9953-82) in Flow

Product Details

14-9953-82

Western Blot (WB)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Neutralization (Neu)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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