Invitrogen

CD184 (CXCR4) Monoclonal Antibody (12G5), eBioscience™

CD184 (CXCR4) Antibody in Flow Cytometry (Flow)

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CD184 (CXCR4) Antibody (14-9999-82) in Flow

Staining of normal human peripheral blood cells with 0.25 µg of Mouse IgG2a K Isotype Control Purified (Product # 14-4724-82) (open histogram) or 0.25 µg of Anti-Human CD184 (CXCR4) Purified (filled histogram) followed by Anti-Mouse IgG Biotin (Product # 13-4013-85) and Streptavidin PE (Product # 12-4317-87).Cells in the lymphocyte gate were used for analysis.

CD184 (CXCR4) Antibody in Western Blot (WB)

CD184 (CXCR4) Antibody in Neutralization (Neu)

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Product Details

14-9999-82

Applications

Tested Dilution

Publications

Western Blot (WB)

View 4 publications 4 publications

Flow Cytometry (Flow)

0.5 µg/test

View 26 publications 26 publications

ELISA (ELISA)

View 1 publication 1 publication

Neutralization (Neu)

Assay-Dependent

View 1 publication 1 publication

Functional Assay (Functional)

Assay-Dependent

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

12G5

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_468685

Product Specific Information

Description: The 12G5 monoclonal antibody reacts with human CD184 (CXCR4), also termed Fusin, LESTR, or HUMSTR. CD184 is a member of the G-protein-coupled chemokine receptor family with seven membrane-spanning domains, and functions as a coreceptor for X4 HIV-1 entry into CD4+ cells. CD184 is expressed predominantly on naive T cell subsets of peripheral blood and is rapidly upregulated by PHA and IL-2 stimulation. The 12G5 antibody shows partial inhibition of chemotaxis and calcium influx induced by SDF-1 (the natural ligand of CD184), blocks CD4-independent HIV-2 infection, and blocks CD4-dependent infection by some T-tropic HIV-1 isolates.

Applications Reported: The 12G5 antibody has been reported for use in flow cytometric analysis. It has also been reported in blocking of CXCR4 in functional studies. (Please use Functional Grade purified 12G5, Product # 16-9999, in functional assays).

Applications Tested: The 12G5 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CXCR4 (Fusin, LESTR, HUMSTR) is a member of the G-protein-coupled chemokine receptor family with seven membrane-spanning domains, and functions as a co-receptor for X4 HIV-1 entry into CD4+ cells. CXCR4 has been proved to be the co-receptor for HIV-2's binding to CD4 through envelope glycoprotein gp 120 and promotes Env-mediated fusion of the virus. In other cases, CXCR4 can even function as the only receptor for HIV-2's binding to the CD4 host cells. CXCR4 is the receptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium ion levels and enhancing MAPK1/MAPK3 activation. CXCR4 acts as a receptor for extracellular ubiquitin; leading to enhanced intracellular calcium ions and reduced cellular cAMP levels. Also, CXCR4 is involved in hematopoiesis and in cardiac ventricular septum formation, and plays an essential role in vascularization of the gastrointestinal tract, probably by regulating vascular branching and/or remodeling processes in endothelial cells. In the CNS, CXCR4 could mediate hippocampal-neuron survival and is involved in cerebellar development. CXCR4 binds bacterial lipopolysaccharide (LPS) and mediates LPS-induced inflammatory response, including TNF secretion by monocytes. Antibodies to CXCR4 block HIV-1 and HIV-2 fusion and infection of human target cells. The amino-terminal domain and the second extracellular loop of CXCR4 serve as HIV biding sites.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Amanita muscaria extract potentiates production of proinflammatory cytokines by dsRNA-activated human microglia.

Frontiers in pharmacology

Wagner A,Pehar M,Yan Z,Kulka M

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 14-9999-82) in Flow Cytometry

Tue May 02 00:00:00 EDT 2023

Airway and Lung Organoids from Human-Induced Pluripotent Stem Cells Can Be Used to Assess CFTR Conductance.

International journal of molecular sciences

Demchenko A,Kondrateva E,Tabakov V,Efremova A,Salikhova D,Bukharova T,Goldshtein D,Balyasin M,Bulatenko N,Amelina E,Lavrov A,Smirnikhina S

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 14-9999-82) in Flow Cytometry

Mon Mar 27 00:00:00 EDT 2023

Dynamics of CXCR4 positive circulating tumor cells in prostate cancer patients during radiotherapy.

International journal of cancer

Klusa D,Lohaus F,Franken A,Baumbach M,Cojoc M,Dowling P,Linge A,Offermann A,Löck S,Hušman D,Rivandi M,Polzer B,Freytag V,Lange T,Neubauer H,Kücken M,Perner S,Hölscher T,Dubrovska A,Krause M,Kurth I,Baumann M,Peitzsch C

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 14-9999-82) in Flow Cytometry

Thu Jun 15 00:00:00 EDT 2023

Parallel functional assessment of m⁶A sites in human endodermal differentiation with base editor screens.

Nature communications

Cheng W,Liu F,Ren Z,Chen W,Chen Y,Liu T,Ma Y,Cao N,Wang J

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 14-9999-82) in Flow Cytometry

Tue Jan 25 00:00:00 EST 2022

Rapamycin Pretreatment Rescues the Bone Marrow AML Cell Elimination Capacity of CAR-T Cells.

Clinical cancer research : an official journal of the American Association for Cancer Research

Nian Z,Zheng X,Dou Y,Du X,Zhou L,Fu B,Sun R,Tian Z,Wei H

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 14-9999-82) in Flow Cytometry

Mon Nov 01 00:00:00 EDT 2021

Cotargeting of miR-126-3p and miR-221-3p inhibits PIK3R2 and PTEN, reducing lung cancer growth and metastasis by blocking AKT and CXCR4 signalling.

Molecular oncology

Di Paolo D,Pontis F,Moro M,Centonze G,Bertolini G,Milione M,Mensah M,Segale M,Petraroia I,Borzi C,Suatoni P,Brignole C,Perri P,Ponzoni M,Pastorino U,Sozzi G,Fortunato O

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 14-9999-82) in Flow Cytometry

Mon Nov 01 00:00:00 EDT 2021

Senescent Tumor Cells Build a Cytokine Shield in Colorectal Cancer.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Choi YW,Kim YH,Oh SY,Suh KW,Kim YS,Lee GY,Yoon JE,Park SS,Lee YK,Park YJ,Kim HS,Park SH,Kim JH,Park TJ

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 14-9999-82) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2021

Longitudinal single-cell profiling reveals molecular heterogeneity and tumor-immune evolution in refractory mantle cell lymphoma.

Nature communications

Zhang S,Jiang VC,Han G,Hao D,Lian J,Liu Y,Zhang R,McIntosh J,Wang R,Dang M,Dai E,Wang Y,Santos D,Badillo M,Leeming A,Chen Z,Hartig K,Bigcal J,Zhou J,Kanagal-Shamanna R,Ok CY,Lee H,Steiner RE,Zhang J,Song X,Nair R,Ahmed S,Rodriquez A,Thirumurthi S,Jain P,Wagner-Bartak N,Hill H,Nomie K,Flowers C,Futreal A,Wang L,Wang M

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 14-9999-82) in Flow Cytometry

Mon May 17 00:00:00 EDT 2021

Associations between Hypertriglyceridemia and Circulating Neutrophil Subpopulation in Patients with Dyslipidemia.

International journal of inflammation

Genkel V,Dolgushin I,Baturina I,Savochkina A,Kuznetsova A,Pykhova L,Shaposhnik I

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 14-9999-82) in Flow Cytometry

Sun Apr 24 00:00:00 EDT 2022

Enhanced CXCR4 Expression of Human CD8^Low T Lymphocytes Is Driven by S1P₄.

Frontiers in immunology

Burkard T,Dreis C,Herrero San Juan M,Huhn M,Weigert A,Pfeilschifter JM,Radeke HH

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 14-9999-82) in Flow Cytometry

Wed Oct 20 00:00:00 EDT 2021

Circulating Progenitor Cells in Patients With Coronary Artery Disease and Renal Insufficiency.

JACC. Basic to translational science

Mehta A,Tahhan AS,Liu C,Dhindsa DS,Nayak A,Hooda A,Moazzami K,Islam SJ,Rogers SC,Almuwaqqat Z,Mokhtari A,Hesaroieh I,Ko YA,Waller EK,Quyyumi AA

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 14-9999-82) in Flow Cytometry

Sat Aug 01 00:00:00 EDT 2020

Optimized RNP transfection for highly efficient CRISPR/Cas9-mediated gene knockout in primary T cells.

The Journal of experimental medicine

Seki A,Rutz S

14-9999 was used in Flow cytometry/Cell sorting to develop an optimised approach for Cas9/RNP transfection of primary mouse and human T cells without TCR stimulation, that results in near complete loss of target gene expression at the population level.

Mon Mar 05 00:00:00 EST 2018

Effect of co‑culture with amniotic epithelial cells on the biological characteristics of amniotic mesenchymal stem cells.

Molecular medicine reports

Ran LJ,Zeng Y,Wang SC,Zhang DS,Hong M,Li SY,Dong J,Shi MX

14-9999-82 was used in Flow Cytometry to investigate the effect of co‚culture with amniotic epithelial cells (AECs) on the biological characteristics of amniotic mesenchymal stem cells.

Sun Jul 01 00:00:00 EDT 2018

Modulatory effects of trophoblast-secreted CXCL12 on the migration and invasion of human first-trimester decidual epithelial cells are mediated by CXCR4 rather than CXCR7.

Reproductive biology and endocrinology : RB&E

Zheng J,Wang H,Zhou W

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 14-9999-82) in Flow Cytometry

Fri Mar 02 00:00:00 EST 2018

The CXCR4 antagonist plerixafor (AMD3100) promotes proliferation of Ewing sarcoma cell lines in vitro and activates receptor tyrosine kinase signaling.

Cell communication and signaling : CCS

Berning P,Schaefer C,Clemens D,Korsching E,Dirksen U,Potratz J

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 14-9999-82) in Flow Cytometry

Fri May 18 00:00:00 EDT 2018

A phase 1 study of the CXCR4 antagonist plerixafor in combination with high-dose cytarabine and etoposide in children with relapsed or refractory acute leukemias or myelodysplastic syndrome: A Pediatric Oncology Experimental Therapeutics Investigators' Consortium study (POE 10-03).

Pediatric blood & cancer

Cooper TM,Sison EAR,Baker SD,Li L,Ahmed A,Trippett T,Gore L,Macy ME,Narendran A,August K,Absalon MJ,Boklan J,Pollard J,Magoon D,Brown PA

14-9999 was used in Flow cytometry/Cell sorting to show Plerixafor, in combination with high-dose cytarabine and etoposide, was well tolerated in children and young adults with relapsed/refractory acute leukemias and MDS.

Tue Aug 01 00:00:00 EDT 2017

Chemokine axes in breast cancer: factors of the tumor microenvironment reshape the CCR7-driven metastatic spread of luminal-A breast tumors.

Journal of leukocyte biology

Weitzenfeld P,Kossover O,Körner C,Meshel T,Wiemann S,Seliktar D,Legler DF,Ben-Baruch A

Wed Jun 01 00:00:00 EDT 2016

ST8SIA4-Dependent Polysialylation is Part of a Developmental Program Required for Germ Layer Formation from Human Pluripotent Stem Cells.

Stem cells (Dayton, Ohio)

Berger RP,Sun YH,Kulik M,Lee JK,Nairn AV,Moremen KW,Pierce M,Dalton S

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 14-9999-82) in Flow Cytometry

Fri Jul 01 00:00:00 EDT 2016

In Vitro Modeling of Alcohol-Induced Liver Injury Using Human-Induced Pluripotent Stem Cells.

Methods in molecular biology (Clifton, N.J.)

Tian L,Prasad N,Jang YY

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 14-9999-82) in Flow Cytometry

Thu Aug 18 00:00:00 EDT 2016

The dual action of poly(ADP-ribose) polymerase -1 (PARP-1) inhibition in HIV-1 infection: HIV-1 LTR inhibition and diminution in Rho GTPase activity.

Frontiers in microbiology

Rom S,Reichenbach NL,Dykstra H,Persidsky Y

14-9999 was used in Functional assays to show that poly(ADP-ribose) polymerase 1 is essential for HIV replication and inhibition may provide an effective approach to management of HIV infection.

Fri Sep 18 00:00:00 EDT 2015

Prevailing role of contact guidance in intrastromal T-cell trapping in human pancreatic cancer.

Clinical cancer research : an official journal of the American Association for Cancer Research

Hartmann N,Giese NA,Giese T,Poschke I,Offringa R,Werner J,Ryschich E

14-9999 was used in Flow cytometry/Cell sorting to analyse how chemokines and extracellular matrix collagen interact in mediating T-cell infiltration in pancreatic ductal adenocarcinoma.

Tue Jul 01 00:00:00 EDT 2014

Antibody epitopes on g protein-coupled receptors mapped with genetically encoded photoactivatable cross-linkers.

Biochemistry

Ray-Saha S,Huber T,Sakmar TP

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 14-9999-82) in Western Blot

Tue Mar 04 00:00:00 EST 2014

Antibody epitopes on g protein-coupled receptors mapped with genetically encoded photoactivatable cross-linkers.

Biochemistry

Ray-Saha S,Huber T,Sakmar TP

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 14-9999-82) in Western Blot

Tue Mar 04 00:00:00 EST 2014

AEG-1 promotes anoikis resistance and orientation chemotaxis in hepatocellular carcinoma cells.

PloS one

Zhou Z,Deng H,Yan W,Luo M,Tu W,Xia Y,He J,Han P,Fu Y,Tian D

14-9999 was used in Western Blotting to define a pathway by which AEG-1 regulates anoikis resistance and orientation chemotaxis during hepatocellular carcinoma cell metastasis.

Thu Oct 08 00:00:00 EDT 2015

Dynamic chemotherapy-induced upregulation of CXCR4 expression: a mechanism of therapeutic resistance in pediatric AML.

Molecular cancer research : MCR

Sison EA,McIntyre E,Magoon D,Brown P

14-9999 was used in Flow cytometry/Cell sorting to show that increased CXCR4 expression after chemotherapy treatment may represent a mechanism of therapeutic resistance in pediatric acute myeloid leukemia.

Sun Sep 01 00:00:00 EDT 2013

Flagellin induces myeloid-derived suppressor cells: implications for Pseudomonas aeruginosa infection in cystic fibrosis lung disease.

Journal of immunology (Baltimore, Md. : 1950)

Rieber N,Brand A,Hector A,Graepler-Mainka U,Ost M,Schäfer I,Wecker I,Neri D,Wirth A,Mays L,Zundel S,Fuchs J,Handgretinger R,Stern M,Hogardt M,Döring G,Riethmüller J,Kormann M,Hartl D

14-9999 was used in Flow cytometry/Cell sorting to demonstrate that flagellin induces the generation of MDSCs and suggest that Pseudomonas aeruginosa uses this mechanism to undermin host defense.

Fri Feb 01 00:00:00 EST 2013

LDL cholesterol modulates human CD34+ HSPCs through effects on proliferation and the IL-17 G-CSF axis.

PloS one

Cimato TR,Palka BA,Lang JK,Young RF

14-9999 was used in Flow cytometry/Cell sorting to determine how cholesterol levels affect haematopoietic stem/progenitor cell quantity in humans.

Sun Jun 15 00:00:00 EDT 2014

Human cytomegalovirus infection inhibits CXCL12- mediated migration and invasion of human extravillous cytotrophoblasts.

Virology journal

Warner JA,Zwezdaryk KJ,Day B,Sullivan DE,Pridjian G,Morris CA

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 14-9999-82) in Neutralization

Thu Nov 01 00:00:00 EDT 2012

Human cytomegalovirus infection inhibits CXCL12- mediated migration and invasion of human extravillous cytotrophoblasts.

Virology journal

Warner JA,Zwezdaryk KJ,Day B,Sullivan DE,Pridjian G,Morris CA

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 14-9999-82) in Neutralization

Thu Nov 01 00:00:00 EDT 2012

Osteopontin enhances the expression and activity of MMP-2 via the SDF-1/CXCR4 axis in hepatocellular carcinoma cell lines.

PloS one

Zhang R,Pan X,Huang Z,Weber GF,Zhang G

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 14-9999-82) in Western Blot

Thu Jan 05 00:00:00 EST 2012

CD137 ligand signaling induces human monocyte to dendritic cell differentiation.

European journal of immunology

Kwajah M M S,Schwarz H

14-9999 was used in Flow cytometry/Cell sorting to deduce that peripheral monocytes can be differentiated into mature inflammatory dendritic cells by the single factor CD137.

Thu Jul 01 00:00:00 EDT 2010

Erythromycin-induced CXCR4 expression on microvascular endothelial cells.

American journal of physiology. Lung cellular and molecular physiology

Takagi Y,Hashimoto N,Phan SH,Imaizumi K,Matsuo M,Nakashima H,Hashimoto I,Hayashi Y,Kawabe T,Shimokata K,Hasegawa Y

14-9999 was used in Flow cytometry to demonstrate that erythromycin markedly induces CXCR4 surface expression on microvascular endothelial cells in vitro and lung capillary endothelial cells in vivo.

Tue Sep 01 00:00:00 EDT 2009

Human mast cell activation with virus-associated stimuli leads to the selective chemotaxis of natural killer cells by a CXCL8-dependent mechanism.

Blood

Burke SM,Issekutz TB,Mohan K,Lee PW,Shmulevitz M,Marshall JS

14-9999 was used in Functional assays to examine the role of mast cells in the recruitment of human natural killer cells to sites of early viral infection via CXCL8.

Sun Jun 15 00:00:00 EDT 2008

The HIV coreceptors CXCR4 and CCR5 are differentially expressed and regulated on human T lymphocytes.

Proceedings of the National Academy of Sciences of the United States of America

Bleul CC,Wu L,Hoxie JA,Springer TA,Mackay CR

Tue Mar 04 00:00:00 EST 1997

Bioinformatics

Protein Aliases: C Cmotif chemokine; C X C motif chemokine; C-X-C chemokine receptor type 4; CC motif chemokine; CCmotif chemokine; CD antigen CD184; CD184; CD184 antigen; chemokine (C-X-C motif) receptor 4; chemokine receptor; CXC; CXC motif chemokine; CXC-R4; FB22; Fusin; HM89; HUMSTR; LAP-3; LCR1; LESTR; Leukocyte-derived seven transmembrane domain receptor; Lipopolysaccharide-associated protein 3; LPS-associated protein 3; neuropeptide Y receptor Y3; neuropeptide Y3 receptor; NPYRL; SDF-1 receptor; seven transmembrane helix receptor; seven-transmembrane-segment receptor, spleen; Stromal cell-derived factor 1 receptor

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Gene Aliases: CD184; CXCR4; D2S201E; FB22; HM89; HSY3RR; LAP-3; LAP3; LCR1; LESTR; NPY3R; NPYR; NPYRL; NPYY3R; WHIM; WHIMS

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UniProt ID: (Human) P61073

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Entrez Gene ID: (Human) 7852

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Function(s)

virus receptor activity actin binding G-protein coupled receptor activity protein binding drug binding coreceptor activity C-X-C chemokine receptor activity cytokine binding ubiquitin protein ligase binding myosin light chain binding ubiquitin binding

Process(es)

activation of MAPK activity response to hypoxia neuron migration epithelial cell development dendritic cell chemotaxis apoptotic process inflammatory response G-protein coupled receptor signaling pathway positive regulation of cytosolic calcium ion concentration neuron recognition response to virus fusion of virus membrane with host plasma membrane calcium-mediated signaling telencephalon cell migration entry into host cell regulation of cell migration regulation of programmed cell death myelin maintenance positive regulation of oligodendrocyte differentiation regulation of chemotaxis regulation of calcium ion transport cardiac muscle contraction chemokine-mediated signaling pathway cellular response to cytokine stimulus

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD184 (CXCR4) Monoclonal Antibody (12G5), eBioscience™

CD184 (CXCR4) Antibody (14-9999-82) in Flow

Product Details

14-9999-82

Western Blot (WB)

Flow Cytometry (Flow)

ELISA (ELISA)

Neutralization (Neu)

Functional Assay (Functional)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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