Invitrogen

CD19 Monoclonal Antibody (HIB19), PE-Cyanine5, eBioscience™

FIGURE: 1 / 33

CD19 Antibody (15-0199-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD3 FITC (Product # 11-0038-42) and Mouse IgG1 K Isotype Control PE-Cyanine5 (Product # 15-4714-81) (left) or Anti-Human CD19 PE-Cyanine5 (right).Cells in the lymphocyte gate were used for analysis.

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Product Details

15-0199-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.25 µg)/test

View 33 publications 33 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-Cyanine5, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

HIB19

Conjugate

PE-Cyanine5 PE-Cyanine5 PE-Cyanine5

Excitation/Emission Max

568/666 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10853658

Product Specific Information

Description: The HIB19 monoclonal antibody reacts with human CD19, a 95 kDa transmembrane glycoprotein. CD19 is expressed by B cells during all stages of development excluding the terminally differentiated plasma cells. Follicular dendritic cells also express CD19. Together CD21, CD81, Leu13, MHC class II, and CD19 form a multimolecular complex that associates with BCR. Signaling through CD19 induces tyrosine phosphorylation, calcium flux and proliferation of B cells. The SJ25C1 antibody and the HIB19 monoclonal antibody recognize overlapping epitopes.

Applications Reported: The HIB19 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This HIB19 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD19 is a member of the immunoglobulin superfamily and has two Ig like domains. The CD19 molecule is expressed on 100% of the peripheral B cells as defined by expression of kappa or lambda light chains. CD19 appears to be expressed on myeloid leukemia cells, particularly those of monocytic lineage. Leukemia phenotype studies have demonstrated that the earliest and broadest B cell restricted antigen is the CD19 antigen. The receptor for CD19 is an important functional regulator of normal and malignant B cell proliferation, and is expressed in all B cell precursor leukemias. Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. CD19 is a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. Besides being a signal-amplifying coreceptor for the B cell receptor (BCR), CD19 can also signal independently of BCR co-ligation and is a central regulatory component upon which multiple signaling pathways converge. Mutation of the CD19 gene results in hypogammaglobulinemia, whereas CD19 overexpression causes B cell hyperactivity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Phenotyping of lymphoproliferative tumours generated in xenografts of non-small cell lung cancer.

Frontiers in oncology

Pearce DR,Akarca AU,De Maeyer RPH,Kostina E,Huebner A,Sivakumar M,Karasaki T,Shah K,Janes SM,McGranahan N,Reddy V,Akbar AN,Moore DA,Marafioti T,Swanton C,Hynds RE

Published figure using CD19 monoclonal antibody (Product # 15-0199-42) in Flow Cytometry

Wed Jul 03 00:00:00 EDT 2024

Human serum albumin promotes self-renewal and expansion of umbilical cord blood CD34⁺ hematopoietic stem/progenitor cells.

Annals of translational medicine

Hua J,Jiao T,Qiao Y,Zhang S,Xiao T,Zhang Y,Yan J

Published figure using CD19 monoclonal antibody (Product # 15-0199-42) in Flow Cytometry

Tue Jan 31 00:00:00 EST 2023

Exosomes derived from stem cells of human deciduous exfoliated teeth inhibit angiogenesis in vivo and in vitro via the transfer of miR-100-5p and miR-1246.

Stem cell research & therapy

Liu P,Zhang Q,Mi J,Wang S,Xu Q,Zhuang D,Chen W,Liu C,Zhang L,Guo J,Wu X

Published figure using CD19 monoclonal antibody (Product # 15-0199-42) in Flow Cytometry

Thu Mar 03 00:00:00 EST 2022

Decoding the pathogenesis of Diamond-Blackfan anemia using single-cell RNA-seq.

Cell discovery

Wang B,Wang C,Wan Y,Gao J,Ma Y,Zhang Y,Tong J,Zhang Y,Liu J,Chang L,Xu C,Shen B,Chen Y,Jiang E,Kurita R,Nakamura Y,Lim KC,Engel JD,Zhou J,Cheng T,Zhu X,Zhu P,Shi L

Published figure using CD19 monoclonal antibody (Product # 15-0199-42) in Flow Cytometry

Tue May 10 00:00:00 EDT 2022

Heterogeneous disease-propagating stem cells in juvenile myelomonocytic leukemia.

The Journal of experimental medicine

Louka E,Povinelli B,Rodriguez-Meira A,Buck G,Wen WX,Wang G,Sousos N,Ashley N,Hamblin A,Booth CAG,Roy A,Elliott N,Iskander D,de la Fuente J,Fordham N,O'Byrne S,Inglott S,Norfo R,Salio M,Thongjuea S,Rao A,Roberts I,Mead AJ

Published figure using CD19 monoclonal antibody (Product # 15-0199-42) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2021

Increased TNF-α Initiates Cytoplasmic Vacuolization in Whole Blood Coculture with Dengue Virus.

Journal of immunology research

Satria RD,Huang TW,Jhan MK,Shen TJ,Tseng PC,Wang YT,Yang ZY,Hsing CH,Lin CF

Published figure using CD19 monoclonal antibody (Product # 15-0199-42) in Flow Cytometry

Mon Dec 06 00:00:00 EST 2021

SARS-CoV-2 infection paralyzes cytotoxic and metabolic functions of the immune cells.

Heliyon

Singh Y,Trautwein C,Fendel R,Krickeberg N,Berezhnoy G,Bissinger R,Ossowski S,Salker MS,Casadei N,Riess O

Published figure using CD19 monoclonal antibody (Product # 15-0199-42) in Flow Cytometry

Tue Jun 01 00:00:00 EDT 2021

Allergen-specific IgE levels and the ability of IgE-allergen complexes to cross-link determine the extent of CD23-mediated T-cell activation.

The Journal of allergy and clinical immunology

Villazala-Merino S,Rodriguez-Dominguez A,Stanek V,Campion NJ,Gattinger P,Hofer G,Froeschl R,Fae I,Lupinek C,Vrtala S,Breiteneder H,Keller W,Perkmann T,Nakamura R,Pickl WF,Valenta R,Eckl-Dorna J,Niederberger V

Published figure using CD19 monoclonal antibody (Product # 15-0199-42) in Flow Cytometry

Sun Mar 01 00:00:00 EST 2020

Human T Cells Expressing a CD19 CAR-T Receptor Provide Insights into Mechanisms of Human CD19-Positive β Cell Destruction.

Cell reports. Medicine

Ma H,Jeppesen JF,Jaenisch R

Published figure using CD19 monoclonal antibody (Product # 15-0199-42) in Flow Cytometry

Tue Sep 22 00:00:00 EDT 2020

Th2 Biased Immunity With Altered B Cell Profiles in Circulation of Patients With Sporotrichosis Caused by Sporothrix globosa.

Frontiers in immunology

Zu J,Yao L,Song Y,Cui Y,Guan M,Chen R,Zhen Y,Li S

Published figure using CD19 monoclonal antibody (Product # 15-0199-42) in Flow Cytometry

Mon May 31 00:00:00 EDT 2021

Influenza Vaccination Primes Human Myeloid Cell Cytokine Secretion and NK Cell Function.

Journal of immunology (Baltimore, Md. : 1950)

Wagstaffe HR,Pickering H,Houghton J,Mooney JP,Wolf AS,Prevatt N,Behrens RH,Holland MJ,Riley EM,Goodier MR

15-0199 was used in Flow cytometry/Cell sorting to study the effects of IL-15 stimulation on the active influenza vaccine and CIML NK cell functions using healthy human adults.

Sun Sep 15 00:00:00 EDT 2019

The Act1 D10N missense variant impairs CD40 signaling in human B-cells.

Genes and immunity

Yu N,Lambert S,Bornstein J,Nair RP,Enerbäck C,Elder JT

Published figure using CD19 monoclonal antibody (Product # 15-0199-42) in Flow Cytometry

Tue Jan 01 00:00:00 EST 2019

Ultra-High-Frequency Reprogramming of Individual Long-Term Hematopoietic Stem Cells Yields Low Somatic Variant Induced Pluripotent Stem Cells.

Cell reports

Wang K,Guzman AK,Yan Z,Zhang S,Hu MY,Hamaneh MB,Yu YK,Tolu S,Zhang J,Kanavy HE,Ye K,Bartholdy B,Bouhassira EE

15-0199-42 was used in Flow Cytometry to suggest LT-HSCs may become the preferred cell source for the production of clinical-grade iPSCs.

Tue Mar 05 00:00:00 EST 2019

A Human IPS Model Implicates Embryonic B-Myeloid Fate Restriction as Developmental Susceptibility to B Acute Lymphoblastic Leukemia-Associated ETV6-RUNX1.

Developmental cell

Böiers C,Richardson SE,Laycock E,Zriwil A,Turati VA,Brown J,Wray JP,Wang D,James C,Herrero J,Sitnicka E,Karlsson S,Smith AJH,Jacobsen SEW,Enver T

Published figure using CD19 monoclonal antibody (Product # 15-0199-42) in Flow Cytometry

Mon Feb 05 00:00:00 EST 2018

IL-15 Promotes Polyfunctional NK Cell Responses to Influenza by Boosting IL-12 Production.

Journal of immunology (Baltimore, Md. : 1950)

Wagstaffe HR,Nielsen CM,Riley EM,Goodier MR

15-0199 was used in Flow cytometry/Cell sorting to suggest that very low concentrations of IL-15 play an important role in boosting accessory cell function to support NK cell effector functions.

Sun Apr 15 00:00:00 EDT 2018

Peli1 negatively regulates noncanonical NF-κB signaling to restrain systemic lupus erythematosus.

Nature communications

Liu J,Huang X,Hao S,Wang Y,Liu M,Xu J,Zhang X,Yu T,Gan S,Dai D,Luo X,Lu Q,Mao C,Zhang Y,Shen N,Li B,Huang M,Zhu X,Jin J,Cheng X,Sun SC,Xiao Y

Published figure using CD19 monoclonal antibody (Product # 15-0199-42) in Flow Cytometry

Mon Mar 19 00:00:00 EDT 2018

NF-κB/MAPK activation underlies ACVR1-mediated inflammation in human heterotopic ossification.

JCI insight

Barruet E,Morales BM,Cain CJ,Ton AN,Wentworth KL,Chan TV,Moody TA,Haks MC,Ottenhoff TH,Hellman J,Nakamura MC,Hsiao EC

15-0199 was used in Flow cytometry/Cell sorting to examine the potential link between bone morphogenetic proteins and inflammation in patients with fibrodysplasia ossificans progressiva.

Thu Nov 15 00:00:00 EST 2018

Human Secretory IgM Emerges from Plasma Cells Clonally Related to Gut Memory B Cells and Targets Highly Diverse Commensals.

Immunity

Magri G,Comerma L,Pybus M,Sintes J,Lligé D,Segura-Garzón D,Bascones S,Yeste A,Grasset EK,Gutzeit C,Uzzan M,Ramanujam M,van Zelm MC,Albero-González R,Vazquez I,Iglesias M,Serrano S,Márquez L,Mercade E,Mehandru S,Cerutti A

Published figure using CD19 monoclonal antibody (Product # 15-0199-42) in Flow Cytometry

Tue Jul 18 00:00:00 EDT 2017

Medial HOXA genes demarcate haematopoietic stem cell fate during human development.

Nature cell biology

Dou DR,Calvanese V,Sierra MI,Nguyen AT,Minasian A,Saarikoski P,Sasidharan R,Ramirez CM,Zack JA,Crooks GM,Galic Z,Mikkola HK

Published figure using CD19 monoclonal antibody (Product # 15-0199-42) in Flow Cytometry

Wed Jun 01 00:00:00 EDT 2016

A Member of the Nuclear Receptor Superfamily, Designated as NR2F2, Supports the Self-Renewal Capacity and Pluripotency of Human Bone Marrow-Derived Mesenchymal Stem Cells.

Stem cells international

Zhu N,Wang H,Wang B,Wei J,Shan W,Feng J,Huang H

Published figure using CD19 monoclonal antibody (Product # 15-0199-42) in Flow Cytometry

Tue Jan 19 00:00:00 EST 2016

CD19 CAR immune pressure induces B-precursor acute lymphoblastic leukaemia lineage switch exposing inherent leukaemic plasticity.

Nature communications

Jacoby E,Nguyen SM,Fountaine TJ,Welp K,Gryder B,Qin H,Yang Y,Chien CD,Seif AE,Lei H,Song YK,Khan J,Lee DW,Mackall CL,Gardner RA,Jensen MC,Shern JF,Fry TJ

Published figure using CD19 monoclonal antibody (Product # 15-0199-42) in Flow Cytometry

Wed Jul 27 00:00:00 EDT 2016

MIF Plays a Key Role in Regulating Tissue-Specific Chondro-Osteogenic Differentiation Fate of Human Cartilage Endplate Stem Cells under Hypoxia.

Stem cell reports

Yao Y,Deng Q,Song W,Zhang H,Li Y,Yang Y,Fan X,Liu M,Shang J,Sun C,Tang Y,Jin X,Liu H,Huang B,Zhou Y

Published figure using CD19 monoclonal antibody (Product # 15-0199-42) in Flow Cytometry

Tue Aug 09 00:00:00 EDT 2016

dNP2 is a blood-brain barrier-permeable peptide enabling ctCTLA-4 protein delivery to ameliorate experimental autoimmune encephalomyelitis.

Nature communications

Lim S,Kim WJ,Kim YH,Lee S,Koo JH,Lee JA,Yoon H,Kim DH,Park HJ,Kim HM,Lee HG,Yun Kim J,Lee JU,Hun Shin J,Kyun Kim L,Doh J,Kim H,Lee SK,Bothwell ALM,Suh M,Choi JM

Published figure using CD19 monoclonal antibody (Product # 15-0199-42) in Flow Cytometry

Tue Sep 15 00:00:00 EDT 2015

Aberrant expression of SALL4 in acute B cell lymphoblastic leukemia: mechanism, function, and implication for a potential novel therapeutic target.

Experimental hematology

Ueno S,Lu J,He J,Li A,Zhang X,Ritz J,Silberstein LE,Chai L

Published figure using CD19 monoclonal antibody (Product # 15-0199-42) in Flow Cytometry

Tue Apr 01 00:00:00 EDT 2014

Induction of type I and type III interferons by Borrelia burgdorferi correlates with pathogenesis and requires linear plasmid 36.

PloS one

Krupna-Gaylord MA,Liveris D,Love AC,Wormser GP,Schwartz I,Petzke MM

Published figure using CD19 monoclonal antibody (Product # 15-0199-42) in Flow Cytometry

Thu Mar 12 00:00:00 EDT 2015

Comparative study of the cytokine/chemokine response in children with differing disease severity in enterovirus 71-induced hand, foot, and mouth disease.

PloS one

Zhang Y,Liu H,Wang L,Yang F,Hu Y,Ren X,Li G,Yang Y,Sun S,Li Y,Chen X,Li X,Jin Q

Published figure using CD19 monoclonal antibody (Product # 15-0199-42) in Flow Cytometry

Tue Jan 21 00:00:00 EST 2014

The acute environment, rather than T cell subset pre-commitment, regulates expression of the human T cell cytokine amphiregulin.

PloS one

Qi Y,Operario DJ,Georas SN,Mosmann TR

15-0199 was used in Flow cytometry/Cell sorting to collate a detailed analysis of the regulation of Amphiregulin expression by human T cell subsets.

Thu Dec 13 00:00:00 EST 2012

Follicular lymphoma tumor-infiltrating T-helper (T(H)) cells have the same polyfunctional potential as normal nodal T(H) cells despite skewed differentiation.

Blood

Hilchey SP,Rosenberg AF,Hyrien O,Secor-Socha S,Cochran MR,Brady MT,Wang JC,Sanz I,Burack WR,Quataert SA,Bernstein SH

15-0199 was used in Flow cytometry/Cell sorting to evaluate the potential of FL-infiltrating CD4(+) T-helper cells to elicit antitumour effects.

Thu Sep 29 00:00:00 EDT 2011

Upregulation of programmed death-1 on T cells and programmed death ligand-1 on monocytes in septic shock patients.

Critical care (London, England)

Zhang Y,Li J,Lou J,Zhou Y,Bo L,Zhu J,Zhu K,Wan X,Cai Z,Deng X

15-0199 was used in Flow cytometry/Cell sorting to demonstrate that lymphocyte apoptosis is markedly increased in septic shock patients, and suggest an essential role for the PD-1/PD-L1 pathway in sepsis-induced immunosuppression.

Wed Dec 07 00:00:00 EST 2011

A pathobiological role of the insulin receptor in chronic lymphocytic leukemia.

Clinical cancer research : an official journal of the American Association for Cancer Research

Saiya-Cork K,Collins R,Parkin B,Ouillette P,Kuizon E,Kujawski L,Erba H,Campagnaro E,Shedden K,Kaminski M,Malek SN

Published figure using CD19 monoclonal antibody (Product # 15-0199-42) in Flow Cytometry

Sun May 01 00:00:00 EDT 2011

Replication of Epstein-Barr virus primary infection in human tonsil tissue explants.

PloS one

Gotoh K,Ito Y,Maruo S,Takada K,Mizuno T,Teranishi M,Nakata S,Nakashima T,Iwata S,Goshima F,Nakamura S,Kimura H

15-0199 was used in Flow cytometry/Cell sorting to assess the ability of B95-8 virus and recombinant Epstein-Barr virus-eGFP to replicate in human lymphoid tissue, to establish a model for the study of viral infection.

Mon Feb 13 00:00:00 EST 2012

Sepsis-induced human lymphocyte apoptosis and cytokine production in "humanized" mice.

Journal of leukocyte biology

Unsinger J,McDonough JS,Shultz LD,Ferguson TA,Hotchkiss RS

Published figure using CD19 monoclonal antibody (Product # 15-0199-42) in Flow Cytometry

Sat Aug 01 00:00:00 EDT 2009

B cells and monocytes from patients with active multiple sclerosis exhibit increased surface expression of both HERV-H Env and HERV-W Env, accompanied by increased seroreactivity.

Retrovirology

Brudek T,Christensen T,Aagaard L,Petersen T,Hansen HJ,Møller-Larsen A

Published figure using CD19 monoclonal antibody (Product # 15-0199-42) in Flow Cytometry

Mon Nov 16 00:00:00 EST 2009

Bioinformatics

Protein Aliases: B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; CD19; Differentiation antigen CD19; Leu-12; T-cell surface antigen Leu-12

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Gene Aliases: B4; CD19; CVID3

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UniProt ID: (Human) P15391

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Entrez Gene ID: (Human) 930

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Function(s)

receptor signaling protein activity protein binding phosphatidylinositol-4,5-bisphosphate 3-kinase activity

Process(es)

cellular defense response cell surface receptor signaling pathway regulation of phosphatidylinositol 3-kinase signaling phosphatidylinositol phosphorylation phosphatidylinositol-mediated signaling regulation of immune response B cell receptor signaling pathway

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD19 Monoclonal Antibody (HIB19), PE-Cyanine5, eBioscience™

CD19 Antibody (15-0199-42) in Flow

Product Details

15-0199-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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