Invitrogen

CD86 (B7-2) Monoclonal Antibody (IT2.2), PE-Cyanine5, eBioscience™

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Antibody Testing Data (1)
Published Figures (22)

CD86 (B7-2) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 23

CD86 (B7-2) Antibody (15-0869-42) in Flow

Staining of normal human peripheral blood cells with Mouse IgG2b Isotype Control PE-Cyanine5 (Product # 15-4732-42) (blue histogram) or Anti-Human CD86 (B7-2) PE-Cyanine5 (purple histogram).Cells in the monocyte gate were used for analysis.

Product Image Gallery

Product Details

15-0869-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.25 µg)/test

View 23 publications 23 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Rhesus monkey

Host/Isotype

Mouse / IgG2b, kappa

Recommended Isotype Control

Mouse IgG2b kappa Isotype Control (eBMG2b), PE-Cyanine5, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

IT2.2

Conjugate

PE-Cyanine5 PE-Cyanine5 PE-Cyanine5

Excitation/Emission Max

568/666 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11042003

Product Specific Information

Description: The IT2.2 monoclonal antibody reacts with human CD86, an ~80 kDa surface receptor also known as B7-2. CD86 and CD80 are members of the B7 family of costimulatory molecules. CD86 is expressed at low levels on B cells, macrophages, and dendritic cells and is upregulated on B cells through a variety of surface stimuli including the BCR complex, CD40 and some cytokine receptors. In addition to CD80 (B7-1), CD86 is a counter-receptor for the T cell surface molecules CD28 and CD152 (CTLA-4). The interaction of CD86 with its ligands plays a critical role in T-B crosstalk, T cell costimulation, autoantibody production and Th2-mediated Ig production. The kinetics of upregulation of CD86 upon stimulation supports its major contribution during the primary phase of an immune response.

Applications Reported: The IT2.2 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This IT2.2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD86 is one of two ligands (the other CD80) for CTLA4 and CD28. CD86 acts as costimulatory molecule in eliciting T-cell help during antigen presentation. Antigen presentation in the absence of sufficient co-stimulation involving CD86/CD80 can induce tolerance. CD80 appears to play a role distinct from CD80 in T helper cell differentiation. CD86 is a type I membrane protein that is a member of the immunoglobulin superfamily. The CD86 protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in two transcript variants encoding different isoforms of CD86. Additional transcript variants have been described for CD86, but their full-length sequences have not been determined. Diseases associated with CD86 dysfunction include gallbladder squamous cell carcinoma and myocarditis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Germline Neurofibromin 1 mutation enhances the anti-tumour immune response and decreases juvenile myelomonocytic leukaemia tumourigenicity.

British journal of haematology

Wang W,Li X,Qin X,Miao Y,Zhang Y,Li S,Yao R,Yang Y,Yu L,Zhu H,Song L,Mao S,Wang X,Chen J,Feng H,Li Y

Published figure using CD86 (B7-2) monoclonal antibody (Product # 15-0869-42) in Flow Cytometry

Sat Jul 01 00:00:00 EDT 2023

Engineered Human Dendritic Cell Exosomes as Effective Delivery System for Immune Modulation.

International journal of molecular sciences

Elsayed R,Elashiry M,Tran C,Yang T,Carroll A,Liu Y,Hamrick M,Cutler CW

Published figure using CD86 (B7-2) monoclonal antibody (Product # 15-0869-42) in Flow Cytometry

Tue Jul 11 00:00:00 EDT 2023

SARS-CoV-2 Delta (B.1.617.2) variant replicates and induces syncytia formation in human induced pluripotent stem cell-derived macrophages.

PeerJ

Thaweerattanasinp T,Wanitchang A,Saenboonrueng J,Srisutthisamphan K,Wanasen N,Sungsuwan S,Jongkaewwattana A,Chailangkarn T

Published figure using CD86 (B7-2) monoclonal antibody (Product # 15-0869-42) in Flow Cytometry

Thu Mar 09 00:00:00 EST 2023

M1 macrophage-derived exosomes and their key molecule lncRNA HOTTIP suppress head and neck squamous cell carcinoma progression by upregulating the TLR5/NF-κB pathway.

Cell death & disease

Jiang H,Zhou L,Shen N,Ning X,Wu D,Jiang K,Huang X

Published figure using CD86 (B7-2) monoclonal antibody (Product # 15-0869-42) in Flow Cytometry

Thu Feb 24 00:00:00 EST 2022

TcpC Inhibits M1 but Promotes M2 Macrophage Polarization via Regulation of the MAPK/NF-κB and Akt/STAT6 Pathways in Urinary Tract Infection.

Cells

Fang J,Ou Q,Wu B,Li S,Wu M,Qiu J,Cen N,Hu K,Che Y,Ma Y,Pan J

Published figure using CD86 (B7-2) monoclonal antibody (Product # 15-0869-42) in Flow Cytometry

Sun Aug 28 00:00:00 EDT 2022

GM-CSF-activated human dendritic cells promote type 1 T follicular helper cell polarization in a CD40-dependent manner.

Journal of cell science

Korniotis S,Saichi M,Trichot C,Hoffmann C,Amblard E,Viguier A,Grondin S,Noel F,Mattoo H,Soumelis V

15-0869-42 was used in Flow cytometry/Cell sorting to report that human blood CD1c+ dendritic cells activated by GM-CSF (also known as CSF2) drive the differentiation of naive CD4+ T cells into Tfh1 cells.

Tue Nov 01 00:00:00 EDT 2022

Glycerol monolaurate inhibition of human B cell activation.

Scientific reports

Fosdick MG,Loftus S,Phillips I,Zacharias ZR,Houtman JCD

Published figure using CD86 (B7-2) monoclonal antibody (Product # 15-0869-42) in Flow Cytometry

Fri Aug 05 00:00:00 EDT 2022

Longitudinal profiling of respiratory and systemic immune responses reveals myeloid cell-driven lung inflammation in severe COVID-19.

Immunity

Szabo PA,Dogra P,Gray JI,Wells SB,Connors TJ,Weisberg SP,Krupska I,Matsumoto R,Poon MML,Idzikowski E,Morris SE,Pasin C,Yates AJ,Ku A,Chait M,Davis-Porada J,Guo XV,Zhou J,Steinle M,Mackay S,Saqi A,Baldwin MR,Sims PA,Farber DL

Published figure using CD86 (B7-2) monoclonal antibody (Product # 15-0869-42) in Flow Cytometry

Tue Apr 13 00:00:00 EDT 2021

Small-molecule inhibitors targeting Polycomb repressive complex 1 RING domain.

Nature chemical biology

Shukla S,Ying W,Gray F,Yao Y,Simes ML,Zhao Q,Miao H,Cho HJ,González-Alonso P,Winkler A,Lund G,Purohit T,Kim E,Zhang X,Ray JM,He S,Nikolaidis C,Ndoj J,Wang J,Jaremko Ł,Jaremko M,Ryan RJH,Guzman ML,Grembecka J,Cierpicki T

Published figure using CD86 (B7-2) monoclonal antibody (Product # 15-0869-42) in Flow Cytometry

Thu Jul 01 00:00:00 EDT 2021

IL-10^-/- Enhances DCs Immunity Against Chlamydia psittaci Infection via OX40L/NLRP3 and IDO/Treg Pathways.

Frontiers in immunology

Li Q,Li X,Quan H,Wang Y,Qu G,Shen Z,He C

Published figure using CD86 (B7-2) monoclonal antibody (Product # 15-0869-42) in Flow Cytometry

Mon Sep 27 00:00:00 EDT 2021

Dendritic Cell Maturation Regulates TSPAN7 Function in HIV-1 Transfer to CD4⁺ T Lymphocytes.

Frontiers in cellular and infection microbiology

Perot BP,García-Paredes V,Luka M,Ménager MM

Published figure using CD86 (B7-2) monoclonal antibody (Product # 15-0869-42) in Flow Cytometry

Wed Mar 17 00:00:00 EDT 2021

Reshaping of the Dendritic Cell Chromatin Landscape and Interferon Pathways during HIV Infection.

Cell host & microbe

Johnson JS,Lucas SY,Amon LM,Skelton S,Nazitto R,Carbonetti S,Sather DN,Littman DR,Aderem A

Published figure using CD86 (B7-2) monoclonal antibody (Product # 15-0869-42) in Flow Cytometry

Wed Mar 14 00:00:00 EDT 2018

AG490 reverses phenotypic alteration of dendritic cells by bladder cancer cells.

Oncology letters

Xiu W,Ma J,Lei T,Zhang M

Published figure using CD86 (B7-2) monoclonal antibody (Product # 15-0869-42) in Flow Cytometry

Sat Sep 01 00:00:00 EDT 2018

Photodynamic therapy reduces the inhibitory effect of osteosarcoma cells on dendritic cells by upregulating HSP70.

Oncology letters

Zhang F,Zhu Y,Fan G,Hu S

Published figure using CD86 (B7-2) monoclonal antibody (Product # 15-0869-42) in Flow Cytometry

Mon Oct 01 00:00:00 EDT 2018

Ginsenoside Rg3 Mitigates Atherosclerosis Progression in Diabetic apoE-/- Mice by Skewing Macrophages to the M2 Phenotype.

Frontiers in pharmacology

Guo M,Xiao J,Sheng X,Zhang X,Tie Y,Wang L,Zhao L,Ji X

Published figure using CD86 (B7-2) monoclonal antibody (Product # 15-0869-42) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2020

Abnormal phenotypic features of IgM+B cell subsets in patients with chronic hepatitis C virus infection.

Experimental and therapeutic medicine

Kong F,Feng B,Zhang H,Rao H,Wang J,Cong X,Wei L

Published figure using CD86 (B7-2) monoclonal antibody (Product # 15-0869-42) in Flow Cytometry

Tue Aug 01 00:00:00 EDT 2017

Leishmania infantum and Leishmania braziliensis: Differences and Similarities to Evade the Innate Immune System.

Frontiers in immunology

Falcão Sde A,Jaramillo TM,Ferreira LG,Bernardes DM,Santana JM,Favali CB

15-0869 was used in Flow cytometry/Cell sorting to investigate the similarities and differences between the ways in which leishmania infantum and leishmania braziliensis evade the innate immune system..

Thu Aug 18 00:00:00 EDT 2016

MicroRNA-720 suppresses M2 macrophage polarization by targeting GATA3.

Bioscience reports

Zhong Y,Yi C

Published figure using CD86 (B7-2) monoclonal antibody (Product # 15-0869-42) in Flow Cytometry

Mon Aug 01 00:00:00 EDT 2016

Omental implantation of BOECs in hemophilia dogs results in circulating FVIII antigen and a complex immune response.

Blood

Ozelo MC,Vidal B,Brown C,Notley C,Hegadorn C,Webster S,Harpell L,Ahlin J,Winterborn A,Handforth J,Arruda VR,Hough C,Lillicrap D

15-0869 was used in Flow cytometry/Cell sorting to investigate the immune response to treatment with FVIII-expressing autologous BOECs.

Thu Jun 26 00:00:00 EDT 2014

Effects of NOD-like receptors in human B lymphocytes and crosstalk between NOD1/NOD2 and Toll-like receptors.

Journal of leukocyte biology

Petterson T,Jendholm J,Månsson A,Bjartell A,Riesbeck K,Cardell LO

15-0869 was used in Flow cytometry/Cell sorting to investigate how NLRs represent a new pathway for B cell activation and a potentially important host defense system against bacterial infections.

Tue Feb 01 00:00:00 EST 2011

Naïve and memory B cells in the rhesus macaque can be differentiated by surface expression of CD27 and have differential responses to CD40 ligation.

Journal of immunological methods

Kuhrt D,Faith S,Hattemer A,Leone A,Sodora D,Picker L,Borghesi L,Cole KS

15-0869 was used in Flow cytometry/Cell sorting to provide evidence that Rhesus macaque naive and memory B cells can be differentiated using surface expression of CD27.

Wed Jan 05 00:00:00 EST 2011

Linking innate and adaptive immunity: human Vgamma9Vdelta2 T cells enhance CD40 expression and HMGB-1 secretion.

Mediators of inflammation

Kalyan S,Chow AW

15-0869 was used in Flow cytometry/Cell sorting to show that gammadelta T cells induce an early upregulation of CD40 on monocytes and the local release of High Mobility Group Box-1.

Wed Dec 16 00:00:00 EST 2009

Mycobacterium tuberculosis-induced gamma interferon production by natural killer cells requires cross talk with antigen-presenting cells involving Toll-like receptors 2 and 4 and the mannose receptor in tuberculous pleurisy.

Infection and immunity

Schierloh P,Yokobori N,Alemán M,Landoni V,Geffner L,Musella RM,Castagnino J,Baldini M,Abbate E,de la Barrera SS,Sasiain MC

15-0869 was used in Flow cytometry/Cell sorting to study how the interplay between M. tuberculosis and NK cells/APC triggering IFN-gamma may play a beneficial role in tuberculous pleurisy by helping to maintain a type 1 profile.

Thu Nov 01 00:00:00 EDT 2007

Bioinformatics

Protein Aliases: Activation B7-2 antigen; B lymphocyte activation antigen B72; B-lymphocyte activation antigen B7-2; B70; BU63; CD28 antigen ligand 2; CD86; CD86 antigen (CD28 antigen ligand 2, B7-2 antigen); CTLA-4 counter-receptor B7.2; Early T-cell co-stimulatory molecule 1; FUN-1; MGC34413; T-lymphocyte activation antigen CD86

View more View less

Gene Aliases: B7-2; B7.2; B70; CD28LG2; CD86; LAB72

View more View less

UniProt ID: (Human) P42081

View more View less

Entrez Gene ID: (Human) 942

View more View less

Function(s)

virus receptor activity receptor activity receptor binding protein binding coreceptor activity phosphatidylinositol-4,5-bisphosphate 3-kinase activity immunoglobulin receptor superfamily

Process(es)

response to yeast adaptive immune response T cell proliferation involved in immune response negative regulation of T cell anergy immune response cell-cell signaling aging positive regulation of cell proliferation regulation of phosphatidylinositol 3-kinase signaling T cell costimulation toll-like receptor 3 signaling pathway response to interferon-gamma positive regulation of activated T cell proliferation T cell activation B cell activation response to drug myeloid dendritic cell differentiation positive regulation of lymphotoxin A biosynthetic process positive regulation of interleukin-2 biosynthetic process positive regulation of interleukin-4 biosynthetic process positive regulation of T-helper 2 cell differentiation positive regulation of transcription, DNA-templated viral entry into host cell phosphatidylinositol phosphorylation phosphatidylinositol-mediated signaling defense response to virus cellular response to lipopolysaccharide cellular response to metal ion cellular response to cytokine stimulus

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CD86 (B7-2) Monoclonal Antibody (IT2.2), PE-Cyanine5, eBioscience™

CD86 (B7-2) Antibody (15-0869-42) in Flow

Product Details

15-0869-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!