Invitrogen

CD90.1 (Thy-1.1) Monoclonal Antibody (HIS51), PE-Cyanine5, eBioscience™

CD90.1 (Thy-1.1) Antibody in Flow Cytometry (Flow)

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CD90.1 (Thy-1.1) Antibody (15-0900-82) in Flow

Heliyon 2022 - Supplemental Figure 2 Identifications of CPSCs. a) CPSCs exhibited spindle-like morphology, chondrogenesis, and osteogenesis. Scale bar = 100 mum. b) Flow cytometric analysis of CD29 and CD90, characteristic positive markers of CPSCs, and the negative marker CD45. c) Morphology of the colony after cells were cultured for 1, 3, 5, 7 days and the colonies were stained with crystal violet. Scale bars: 200 mum ... View More

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Product Details

15-0900-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.06 µg/test

View 38 publications 38 publications

Product Specifications

Species Reactivity

Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG2a, kappa

Recommended Isotype Control

Mouse IgG2a kappa Isotype Control (eBM2a), PE-Cyanine5, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

HIS51

Conjugate

PE-Cyanine5 PE-Cyanine5 PE-Cyanine5

Excitation/Emission Max

568/666 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_468781

Product Specific Information

Description: The HIS51 monoclonal antibody reacts with rat CD90 and cross-reacts with mouse CD90.1, a GPI-linked membrane molecule. In the rat, CD90 is expressed by hematopoietic stem cells, immature B cells, thymocytes, recent thymic emigrants, neurons, inflammed endothelia and other cell types. In the CD90.1-expressing mouse strains, PL and AKR, CD90 is expressed by early hematopoietic cells in the bone marrow, thymocytes and mature T cells.

Applications Reported: The HIS51 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The HIS51 antibody has been tested by flow cytometric analysis of rat thymocyte and splenocyte suspensions. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD90 (Thy 1) antigen is a GPI linked glycoprotein member of the Immunoglobulin super family. It is expressed in murine T cells, thymocytes, neural cells, Kupffer's cells and fibroblasts. Thy 1 may play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Higher throughput workflow with sensitive, reliable and automatic quantification of myelination in vitro suitable for drug screening.

Scientific reports

Seiler S,Wälti CM,de Barros V,Barbash S,Foo LC

15-0900-82 was used in flow cytometry experiments to develop a method allowing systematic and reliable testing of remyelinating compound efficacy.

Sat Feb 18 00:00:00 EST 2023

Regenerative Effects of Locally or Intra-Arterially Administered BMSCs on the Thin Endometrium.

Frontiers in bioengineering and biotechnology

Guo Q,Chang Y,Li J,Zhou C,Huang R,Yang X,Liu G,Liang X

Published figure using CD90.1 (Thy-1.1) monoclonal antibody (Product # 15-0900-82) in Flow Cytometry

Sat Jul 16 00:00:00 EDT 2022

Sustained release of exosomes loaded into polydopamine-modified chitin conduits promotes peripheral nerve regeneration in rats.

Neural regeneration research

Li C,Liu SY,Zhang M,Pi W,Wang B,Li QC,Lu CF,Zhang PX

Published figure using CD90.1 (Thy-1.1) monoclonal antibody (Product # 15-0900-82) in Flow Cytometry

Thu Sep 01 00:00:00 EDT 2022

NLRP3 downregulation enhances engraftment and functionality of adipose-derived stem cells to alleviate erectile dysfunction in diabetic rats.

Frontiers in endocrinology

Luo C,Peng Y,Zhou X,Fan J,Chen W,Zhang H,Wei A

Published figure using CD90.1 (Thy-1.1) monoclonal antibody (Product # 15-0900-82) in Flow Cytometry

Tue Aug 09 00:00:00 EDT 2022

Binary outcomes of enhancer activity underlie stable random monoallelic expression.

eLife

Kissiov DU,Ethell A,Chen S,Wolf NK,Zhang C,Dang SM,Jo Y,Madsen KN,Paranjpe I,Lee AY,Chim B,Muljo SA,Raulet DH

Published figure using CD90.1 (Thy-1.1) monoclonal antibody (Product # 15-0900-82) in Flow Cytometry

Thu May 26 00:00:00 EDT 2022

Propofol suppresses adipose-derived stem cell progression via PI3K/AKT-Wnt signaling pathway.

BMC anesthesiology

Yin G,Wang J,Zhong Y,Wu W

Published figure using CD90.1 (Thy-1.1) monoclonal antibody (Product # 15-0900-82) in Flow Cytometry

Wed Mar 09 00:00:00 EST 2022

Single-cell transcriptomics reveals variable trajectories of CSPCs in the progression of osteoarthritis.

Heliyon

Qi L,Wang J,Chen X,Ding Y,Ling B,Wang W,Xu J,Xue Z

Published figure using CD90.1 (Thy-1.1) monoclonal antibody (Product # 15-0900-82) in Flow Cytometry

Tue Nov 01 00:00:00 EDT 2022

Macrophage polarization by MSC-derived CXCL12 determines tumor growth.

Cellular & molecular biology letters

Babazadeh S,Nassiri SM,Siavashi V,Sahlabadi M,Hajinasrollah M,Zamani-Ahmadmahmudi M

Published figure using CD90.1 (Thy-1.1) monoclonal antibody (Product # 15-0900-82) in Flow Cytometry

Sat Jun 26 00:00:00 EDT 2021

Effects of combination treatment with transcranial magnetic stimulation and bone marrow mesenchymal stem cell transplantation or Raf inhibition on spinal cord injury in rats.

Molecular medicine reports

Feng S,Wang S,Sun S,Su H,Zhang L

Published figure using CD90.1 (Thy-1.1) monoclonal antibody (Product # 15-0900-82) in Flow Cytometry

Thu Apr 01 00:00:00 EDT 2021

Adipose mesenchymal stem cells combined with platelet-rich plasma accelerate diabetic wound healing by modulating the Notch pathway.

Stem cell research & therapy

Ebrahim N,Dessouky AA,Mostafa O,Hassouna A,Yousef MM,Seleem Y,El Gebaly EAEAM,Allam MM,Farid AS,Saffaf BA,Sabry D,Nawar A,Shoulah AA,Khalil AH,Abdalla SF,El-Sherbiny M,Elsherbiny NM,Salim RF

Published figure using CD90.1 (Thy-1.1) monoclonal antibody (Product # 15-0900-82) in Flow Cytometry

Tue Jul 13 00:00:00 EDT 2021

A genome-wide CRISPR/Cas9 screen to identify phagocytosis modulators in monocytic THP-1 cells.

Scientific reports

Lindner B,Martin E,Steininger M,Bundalo A,Lenter M,Zuber J,Schuler M

Published figure using CD90.1 (Thy-1.1) monoclonal antibody (Product # 15-0900-82) in Flow Cytometry

Mon Jun 21 00:00:00 EDT 2021

Manufacturing and banking canine adipose-derived mesenchymal stem cells for veterinary clinical application.

BMC veterinary research

Luo H,Li D,Chen Z,Wang B,Chen S

Published figure using CD90.1 (Thy-1.1) monoclonal antibody (Product # 15-0900-82) in Flow Cytometry

Mon Mar 01 00:00:00 EST 2021

Icariin Promote Stem Cells Regeneration and Repair Acinar Cells in L-arginine / Radiation -Inducing Chronic Pancreatitis in Rats.

Dose-response : a publication of International Hormesis Society

Moustafa EM,Moawed FSM,Abdel-Hamid GR

Published figure using CD90.1 (Thy-1.1) monoclonal antibody (Product # 15-0900-82) in Flow Cytometry

Mon Apr 18 00:00:00 EDT 2022

Apoptotic bodies derived from mesenchymal stem cells promote cutaneous wound healing via regulating the functions of macrophages.

Stem cell research & therapy

Liu J,Qiu X,Lv Y,Zheng C,Dong Y,Dou G,Zhu B,Liu A,Wang W,Zhou J,Liu S,Liu S,Gao B,Jin Y

Published figure using CD90.1 (Thy-1.1) monoclonal antibody (Product # 15-0900-82) in Flow Cytometry

Fri Nov 27 00:00:00 EST 2020

Effects of bone marrow‑derived mesenchymal stem cell transplantation on chronic obstructive pulmonary disease/obstructive sleep apnea overlap syndrome in rats.

Molecular medicine reports

Chen M,Huang Z,Bi H,Pan X,He J,He L,He X,Du J,Zhou K,Wang L,Wang Q,Guo X,Jin Z

Published figure using CD90.1 (Thy-1.1) monoclonal antibody (Product # 15-0900-82) in Flow Cytometry

Fri Nov 01 00:00:00 EDT 2019

Co-culture of the bone and bone marrow: a novel way to obtain mesenchymal stem cells with enhanced osteogenic ability for fracture healing in SD rats.

Journal of orthopaedic surgery and research

Zhu C,Sha M,Jiang H,Lin J,Lin W,Li W,Chen X,Huang G,Ding Z

Published figure using CD90.1 (Thy-1.1) monoclonal antibody (Product # 15-0900-82) in Flow Cytometry

Tue Sep 03 00:00:00 EDT 2019

Protective mechanism of artemisinin on rat bone marrow-derived mesenchymal stem cells against apoptosis induced by hydrogen peroxide via activation of c-Raf-Erk1/2-p90^rsk-CREB pathway.

Stem cell research & therapy

Fang J,Zhao X,Li S,Xing X,Wang H,Lazarovici P,Zheng W

Published figure using CD90.1 (Thy-1.1) monoclonal antibody (Product # 15-0900-82) in Flow Cytometry

Sat Oct 26 00:00:00 EDT 2019

Tanshinone IIA and Astragaloside IV promote the angiogenesis of mesenchymal stem cell-derived endothelial cell-like cells via upregulation of Cx37, Cx40 and Cx43.

Experimental and therapeutic medicine

Li Z,Zhang S,Cao L,Li W,Ye YC,Shi ZX,Wang ZR,Sun LX,Wang JW,Jia LT,Wang W

Published figure using CD90.1 (Thy-1.1) monoclonal antibody (Product # 15-0900-82) in Flow Cytometry

Thu Feb 01 00:00:00 EST 2018

Tail Vein Infusion of Adipose-Derived Mesenchymal Stem Cell Alleviated Inflammatory Response and Improved Blood Brain Barrier Condition by Suppressing Endoplasmic Reticulum Stress in a Middle Cerebral Artery Occlusion Rat Model.

Medical science monitor : international medical journal of experimental and clinical research

Chi L,Huang Y,Mao Y,Wu K,Zhang L,Nan G

Published figure using CD90.1 (Thy-1.1) monoclonal antibody (Product # 15-0900-82) in Flow Cytometry

Mon Jun 11 00:00:00 EDT 2018

Stability and function of regulatory T cells expressing the transcription factor T-bet.

Nature

Levine AG,Mendoza A,Hemmers S,Moltedo B,Niec RE,Schizas M,Hoyos BE,Putintseva EV,Chaudhry A,Dikiy S,Fujisawa S,Chudakov DM,Treuting PM,Rudensky AY

Published figure using CD90.1 (Thy-1.1) monoclonal antibody (Product # 15-0900-82) in Flow Cytometry

Thu Jun 15 00:00:00 EDT 2017

Mutations in 5-methylcytosine oxidase TET2 and RhoA cooperatively disrupt T cell homeostasis.

The Journal of clinical investigation

Zang S,Li J,Yang H,Zeng H,Han W,Zhang J,Lee M,Moczygemba M,Isgandarova S,Yang Y,Zhou Y,Rao A,You MJ,Sun D,Huang Y

Published figure using CD90.1 (Thy-1.1) monoclonal antibody (Product # 15-0900-82) in Flow Cytometry

Tue Aug 01 00:00:00 EDT 2017

CD73-derived adenosine and tenascin-C control cytokine production by epicardium-derived cells formed after myocardial infarction.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Hesse J,Leberling S,Boden E,Friebe D,Schmidt T,Ding Z,Dieterich P,Deussen A,Roderigo C,Rose CR,Floss DM,Scheller J,Schrader J

Published figure using CD90.1 (Thy-1.1) monoclonal antibody (Product # 15-0900-82) in Flow Cytometry

Sat Jul 01 00:00:00 EDT 2017

A new scaffold containing small intestinal submucosa and mesenchymal stem cells improves pancreatic islet function and survival in vitro and in vivo.

International journal of molecular medicine

Wang D,Ding X,Xue W,Zheng J,Tian X,Li Y,Wang X,Song H,Liu H,Luo X

Published figure using CD90.1 (Thy-1.1) monoclonal antibody (Product # 15-0900-82) in Flow Cytometry

Sun Jan 01 00:00:00 EST 2017

Yi Guan Jian decoction may enhance hepatic differentiation of bone marrow‑derived mesenchymal stem cells via SDF‑1 in vitro.

Molecular medicine reports

Fu LL,Pang BY,Zhu Y,Wang L,Leng AJ,Chen HL

Published figure using CD90.1 (Thy-1.1) monoclonal antibody (Product # 15-0900-82) in Flow Cytometry

Fri Sep 01 00:00:00 EDT 2017

Tissue-engineered sling with adipose-derived stem cells under static mechanical strain.

Experimental and therapeutic medicine

Wang Y,Wang W,Wang X,Wang Y,Wang J,Fu Q,Shi G

Published figure using CD90.1 (Thy-1.1) monoclonal antibody (Product # 15-0900-82) in Flow Cytometry

Tue Aug 01 00:00:00 EDT 2017

Effects of nerve growth factor and basic fibroblast growth factor dual gene modification on rat bone marrow mesenchymal stem cell differentiation into neuron-like cells in vitro.

Molecular medicine reports

Hu Y,Zhang Y,Tian K,Xun C,Wang S,Lv D

Published figure using CD90.1 (Thy-1.1) monoclonal antibody (Product # 15-0900-82) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2016

Treatment of radiation-induced acute intestinal injury with bone marrow-derived mesenchymal stem cells.

Experimental and therapeutic medicine

Zheng K,Wu W,Yang S,Huang L,Chen J,Gong C,Fu Z,Lin R,Tan J

Published figure using CD90.1 (Thy-1.1) monoclonal antibody (Product # 15-0900-82) in Flow Cytometry

Wed Jun 01 00:00:00 EDT 2016

Protective effect of adipose-derived mesenchymal stem cells against acute kidney injury induced by ischemia-reperfusion in Sprague-Dawley rats.

Experimental and therapeutic medicine

Sheashaa H,Lotfy A,Elhusseini F,Aziz AA,Baiomy A,Awad S,Alsayed A,El-Gilany AH,Saad MA,Mahmoud K,Zahran F,Salem DA,Sarhan A,Ghaffar HA,Sobh M

15-0900 was used in Flow cytometry/Cell sorting to study the differentiation capabilities of adipose-derived mesenchymal stem cells in response to ischemia-reperfusion injury.

Sun May 01 00:00:00 EDT 2016

Identification of a CD133-CD55- population functions as a fetal common skeletal progenitor.

Scientific reports

Weng L,Hu X,Kumar B,Garcia M,Todorov I,Jung X,Marcucci G,Forman SJ,Chen CC

Published figure using CD90.1 (Thy-1.1) monoclonal antibody (Product # 15-0900-82) in Flow Cytometry

Thu Dec 08 00:00:00 EST 2016

Fibroblast Growth Factor-10 (FGF-10) Mobilizes Lung-resident Mesenchymal Stem Cells and Protects Against Acute Lung Injury.

Scientific reports

Tong L,Zhou J,Rong L,Seeley EJ,Pan J,Zhu X,Liu J,Wang Q,Tang X,Qu J,Bai C,Song Y

Published figure using CD90.1 (Thy-1.1) monoclonal antibody (Product # 15-0900-82) in Flow Cytometry

Fri Feb 12 00:00:00 EST 2016

Isolation of adipose and bone marrow mesenchymal stem cells using CD29 and CD90 modifies their capacity for osteogenic and adipogenic differentiation.

Journal of tissue engineering

Davies OG,Cooper PR,Shelton RM,Smith AJ,Scheven BA

Published figure using CD90.1 (Thy-1.1) monoclonal antibody (Product # 15-0900-82) in Flow Cytometry

Fri Sep 18 00:00:00 EDT 2015

Distinct in vitro properties of embryonic and extraembryonic fibroblast-like cells are reflected in their in vivo behavior following grafting in the adult mouse brain.

Cell transplantation

Costa R,Bergwerf I,Santermans E,De Vocht N,Praet J,Daans J,Le Blon D,Hoornaert C,Reekmans K,Hens N,Goossens H,Berneman Z,Parolini O,Alviano F,Ponsaerts P

Published figure using CD90.1 (Thy-1.1) monoclonal antibody (Product # 15-0900-82) in Flow Cytometry

Mon Nov 16 00:00:00 EST 2015

Mesenchymal Cancer Cell-Stroma Crosstalk Promotes Niche Activation, Epithelial Reversion, and Metastatic Colonization.

Cell reports

Del Pozo Martin Y,Park D,Ramachandran A,Ombrato L,Calvo F,Chakravarty P,Spencer-Dene B,Derzsi S,Hill CS,Sahai E,Malanchi I

Published figure using CD90.1 (Thy-1.1) monoclonal antibody (Product # 15-0900-82) in Flow Cytometry

Tue Dec 22 00:00:00 EST 2015

Injury-dependent retention of intraportally administered mesenchymal stromal cells following partial hepatectomy of steatotic liver does not lead to improved liver recovery.

PloS one

Boeykens N,Ponsaerts P,Van der Linden A,Berneman Z,Ysebaert D,De Greef K

Published figure using CD90.1 (Thy-1.1) monoclonal antibody (Product # 15-0900-82) in Flow Cytometry

Thu Feb 13 00:00:00 EST 2014

Inefficient TLR4/MD-2 heterotetramerization by monophosphoryl lipid A.

PloS one

Casella CR,Mitchell TC

Published figure using CD90.1 (Thy-1.1) monoclonal antibody (Product # 15-0900-82) in Flow Cytometry

Mon Nov 11 00:00:00 EST 2013

Acid ceramidase maintains the chondrogenic phenotype of expanded primary chondrocytes and improves the chondrogenic differentiation of bone marrow-derived mesenchymal stem cells.

PloS one

Simonaro CM,Sachot S,Ge Y,He X,Deangelis VA,Eliyahu E,Leong DJ,Sun HB,Mason JB,Haskins ME,Richardson DW,Schuchman EH

Published figure using CD90.1 (Thy-1.1) monoclonal antibody (Product # 15-0900-82) in Flow Cytometry

Mon Nov 11 00:00:00 EST 2013

Enhanced development of CD4+ gammadelta T cells in the absence of Itk results in elevated IgE production.

Blood

Qi Q,Xia M,Hu J,Hicks E,Iyer A,Xiong N,August A

15-0900 was used in Flow cytometry/Cell sorting to investigate the effect of Itk on T cell populations, showing that development of CD4+ gammadelta T cells is enhanced and IgE production is elevated in the absence of Itk.

Thu Jul 16 00:00:00 EDT 2009

Tracking epitope-specific T cells.

Nature protocols

Moon JJ,Chu HH,Hataye J,Pagán AJ,Pepper M,McLachlan JB,Zell T,Jenkins MK

Published figure using CD90.1 (Thy-1.1) monoclonal antibody (Product # 15-0900-82) in Flow Cytometry

Tue Jun 30 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: CD90; FLJ33325; Thy 1.2; Thy-1 antigen; thy-1 glycoprotein; Thy-1 membrane glycoprotein; Thy-1 protein; thymus cell antigen 1, theta; Thymus cell surface antigen

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Gene Aliases: CD7; CD90; T25; Thy-1; Thy-1.2; Thy1; Thy1.1; Thy1.2

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UniProt ID: (Mouse) P01831, (Rat) P01830

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Entrez Gene ID: (Mouse) 21838, (Rat) 24832

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Function(s)

GTPase activator activity integrin binding enzyme binding protein kinase binding GPI anchor binding protein binding cell adhesion molecule

Process(es)

angiogenesis negative regulation of protein kinase activity cytoskeleton organization single organismal cell-cell adhesion negative regulation of cell migration positive regulation of GTPase activity retinal cone cell development protein autophosphorylation focal adhesion assembly negative regulation of axonogenesis T cell receptor signaling pathway negative regulation of T cell receptor signaling pathway positive regulation of T cell activation positive regulation of release of sequestered calcium ion into cytosol negative regulation of apoptotic process mast cell activation negative regulation of fibroblast proliferation positive regulation of peptidyl-tyrosine phosphorylation

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CD90.1 (Thy-1.1) Monoclonal Antibody (HIS51), PE-Cyanine5, eBioscience™

CD90.1 (Thy-1.1) Antibody (15-0900-82) in Flow

Product Details

15-0900-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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