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Invitrogen
CD88 (C5aR1) Monoclonal Antibody (S5/1), Functional Grade, eBioscience™
Product Details
16-0888-85
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
S5/1
Immunogen
BSA-Conjugated Peptide aa. 1-31
Conjugate
Form
Liquid
Concentration
1.0 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
no preservative
Storage conditions
4° C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2815074
Product Specific Information
Description: This S5/1 monoclonal antibody recognizes human CD88 also known as C5aR1. C5aR1 is a complement receptor widely expressed on cells of the myeloid lineage. Clone S5/1 has been reported to block C5a binding.
Applications Reported: This S5/1 antibody has been reported for use in flow cytometric analysis. S5/1 has also been reported for blocking activity.
Applications Tested: This S5/1 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Storage and handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: Greater than 90%, as determined by SDS-PAGE.
Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.
Aggregation: Less than 10%, as determined by HPLC.
Target Information
CD88, also known as C5aR1, is a C5a Anaphylatoxin Receptor that mediates the effects of one of the most potent chemotactic agents known. C5AR1 has been implicated in asthma, and its expression has been reported in myeloid blood cells, brain, liver, lung, spleen, heart, kidney, and intestinal tract. CD88 is the receptor for the chemotactic and inflammatory peptide anaphylatoxin C5a. Functionally, CD88 stimulates chemotaxis, granule enzyme release and superoxide anion production. CD88 is also a component of the classical pathway of the complement cascade; it may be involved with tissue injury associated with ischemia or sepsis and it may be a component of the membrane attack complex. Diseases associated with CD88 include Mannose-Binding Lectin Deficiency and Systemic Lupus Erythematosus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C5a anaphylatoxin chemotactic receptor; C5a anaphylatoxin chemotactic receptor 1; C5a anaphylatoxin receptor; C5a ligand; C5a Receptor; C5a-R; CD88; CD88 antigen; complement component 5 receptor 1
Gene Aliases: C5A; C5AR; C5AR1; C5R1; CD88
UniProt ID: (Human) P21730
Entrez Gene ID: (Human) 728
activation of MAPK activity
apoptotic process
chemotaxis
inflammatory response
immune response
cellular defense response
signal transduction
activation of phospholipase C activity
positive regulation of cytosolic calcium ion concentration
sensory perception of chemical stimulus
positive regulation of vascular endothelial growth factor production
positive regulation of macrophage chemotaxis
cell proliferation in hindbrain
regulation of complement activation
neutrophil chemotaxis
organ regeneration
response to peptidoglycan
response to lipopolysaccharide
complement component C5a signaling pathway
mRNA transcription from RNA polymerase II promoter
negative regulation of neuron apoptotic process
positive regulation of angiogenesis
positive regulation of epithelial cell proliferation
defense response to Gram-positive bacterium
positive regulation of ERK1 and ERK2 cascade
positive regulation of neutrophil chemotaxis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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