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Invitrogen
CD314 (NKG2D) Monoclonal Antibody (1D11), Functional Grade, eBioscience™
Product Details
16-5878-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Not Applicable
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
1D11
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
no preservative
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_469116
Product Specific Information
Description: The 1D11 monoclonal antibody reacts with the human NKG2D, a 42 kDa lectin-like molecule expressed by NK cells, gamma delta T cells, CD8+ T cells, and some CD4+ T cells. Human NKG2D forms complexes with DAP10, a membrane adaptor protein, and has the ability to costimulate multiple NK activation receptors. The counter-receptor for human NKG2D has been identified as MICA/MICB expressed on epithelial tumors from lung, breast, kidney, ovary, prostate and colon carcinoma. 5C6 and 1D11 block binding of soluble MICA to gamma delta TCR T cell clones and inhibit lysis by these cells. 5C6 and 1D11 induced NKG2D function of redirected lysis of FcReceptor bearing P815 cells.
Applications Reported: The 1D11 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining. It has also been reported in inhibition of ligand binding in in vitro assays. (Fluorochrome-conjugated 1D11 is recommended for use in flow cytometric analysis).
Applications Tested: This 1D11 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Storage and handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: Greater than 90%, as determined by SDS-PAGE.
Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.
Aggregation: Less than 10%, as determined by HPLC.
Target Information
Natural killer (NK) cells are lymphocytes that can mediate lysis of certain tumor cells and virus-infected cells without previous activation. NK cells can regulate specific humoral and cell-mediated immunity, and preferentially express several calcium-dependent (C-type) lectins, which have been implicated in the regulation of NK cell function. The NK gene encodes a member of the NKG2 family, and the encoded transmembrane protein is characterized by a type II membrane orientation (extracellular C terminus) and the presence of a C-type lectin domain. The NKG2 gene family is located within the NK complex, a region that contains several C-type lectin genes preferentially expressed in NK cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD314; Killer cell lectin-like receptor subfamily K member 1; killer cell lectin-like receptor subfamily K, member 1; natural killer cell receptor; NK cell receptor D; NKG2-D type II integral membrane protein; NKG2-D-activating NK receptor
Gene Aliases: CD314; D12S2489E; KLR; KLRK1; NKG2-D; NKG2D
UniProt ID: (Human) P26718
Entrez Gene ID: (Human) 22914
stimulatory C-type lectin receptor signaling pathway
adaptive immune response
positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target
signal transduction
natural killer cell activation
cell differentiation
positive regulation of myeloid dendritic cell activation
T cell costimulation
positive regulation of interferon-gamma production
negative regulation of GTPase activity
positive regulation of apoptotic process
innate immune response
positive regulation of nitric oxide biosynthetic process
positive regulation of natural killer cell mediated cytotoxicity
regulation of immune response
defense response to Gram-positive bacterium
cellular response to lipopolysaccharide
negative regulation of natural killer cell chemotaxis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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