Invitrogen

IFNAR1 Monoclonal Antibody (MAR1-5A3), Functional Grade, eBioscience™

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IFNAR1 Antibody (16-5945-85) in Neu

Cell reports 2022 - Figure 4. RIPK1-mediated IFN production suppresses virus-induced cell death (A-D) BMDMs were pre-treated with 10 mug/mL anti-IFNAR1 blocking antibody or IgG for 1 h (A and B) or left untreated (C and D), then infected with MNV-1 (MOI = 10) in vitro . Real-time cell death was monitored by Sytox Green staining and quantification (A and C). Mean + SEM shown. ****p < 0.001 (two-way ANOVA). (B and D) Representat... View More

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Product Details

16-5945-85

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

View 1 publication 1 publication

ELISA (ELISA)

View 2 publications 2 publications

Neutralization (Neu)

Assay-Dependent

View 6 publications 6 publications

Functional Assay (Functional)

Assay-Dependent

View 7 publications 7 publications

Inhibition Assays (IA)

View 1 publication 1 publication

Blocking Assay (BLOCK)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Bacteria, Mouse

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

MAR1-5A3

Conjugate

Functional Grade Functional Grade Functional Grade

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1210688

Product Specific Information

Description: The MAR1-5A3 monoclonal antibody reacts with mouse IFN alpha/beta receptor subunit 1 (IFNAR-1). The type I IFN receptor subunit 1 is co-expressed with IFNAR-2 on nearly all cells and make up the heterodimeric receptor complex that binds to all type I IFNs, IFN alpha and IFN beta. Type I IFNs are a group of structurally and functionally related proteins that demonstrate antiviral, antiparasitic, and antiproliferative activities.

Applications Reported: The MAR1-5A3 antibody has been reported for use in neutralization of IFNAR1 bioactivity.

Applications Tested: The Functional Grade Purified MAR1-5A3 antibody has been tested by LAL assay to verify low endotoxin levels, by bioassay for neutralization of mouse IFNAR-1bioactivity. The MAR1-5A3 antibody at 0.04 µg/mL has been found to inhibit by 50% the biological effects of 0.5 ng/mL mouse IFN-a2 (ND50), in an EMCV assay of L929 cell protection. Detailed information and protocols about cytokine bioassays and in vitro cytokine neutralization using antibodies can be found in Best Protocols® section.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

Target Information

Interferons are widely used therapeutic agents because of their anti tumor and antiviral effects and because of their modulatory effects on the immune system (Biron,2001, Kirkwood, 2002). These cytokines produce their effects by binding to the Type 1 Interferon-& Receptor (IFNAR1). Down regulation of this receptor plays a key role in determining the magnitude and duration of cytokine signaling. This down regulation is thought to be influenced by phosphorylation of Serine 535 and 539 in the IFNAR1 (Kumar et al., 2003).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Epigenetic state determines the in vivo efficacy of STING agonist therapy.

Nature communications

Falahat R,Berglund A,Perez-Villarroel P,Putney RM,Hamaidi I,Kim S,Pilon-Thomas S,Barber GN,Mulé JJ

16-5945-85 was used in Blocking Assay to show that reversal of methylation silencing of STING in murine melanoma cell lines using a clinically available DNA methylation inhibitor can improve agonist-induced STING activation and type-I IFN induction, which, in tumor-bearing mice, can induce tumor regression through a CD8+ T cell-dependent immune response.

Wed Mar 22 00:00:00 EDT 2023

Molecular mechanism of RIPK1 and caspase-8 in homeostatic type I interferon production and regulation.

Cell reports

Wang Y,Karki R,Mall R,Sharma BR,Kalathur RC,Lee S,Kancharana B,So M,Combs KL,Kanneganti TD

Published figure using IFNAR1 monoclonal antibody (Product # 16-5945-85) in Neutralization

Tue Oct 04 00:00:00 EDT 2022

Type I interferon signaling mediates Mycobacterium tuberculosis-induced macrophage death.

The Journal of experimental medicine

Zhang L,Jiang X,Pfau D,Ling Y,Nathan CF

16-5945 was used in Flow cytometry/Cell sorting to study how autocrine or paracrine signalling by macrophage-derived type I interferons play a critical role in the death of Mycobacterium tuberculosis-infected macrophages.

Mon Feb 01 00:00:00 EST 2021

Type I interferon signaling mediates Mycobacterium tuberculosis-induced macrophage death.

The Journal of experimental medicine

Zhang L,Jiang X,Pfau D,Ling Y,Nathan CF

Mon Feb 01 00:00:00 EST 2021

Type I interferon decreases macrophage energy metabolism during mycobacterial infection.

Cell reports

Olson GS,Murray TA,Jahn AN,Mai D,Diercks AH,Gold ES,Aderem A

16-5945-85 was used in Functional assay to suggest that a type I IFN-mitochondrial feedback loop controls macrophage responses to mycobacteria and that this could contribute to pathogenesis across a range of diseases.

Tue Jun 01 00:00:00 EDT 2021

Bacterial-induced cell fusion is a danger signal triggering cGAS-STING pathway via micronuclei formation.

Proceedings of the National Academy of Sciences of the United States of America

Ku JWK,Chen Y,Lim BJW,Gasser S,Crasta KC,Gan YH

16-5945-38 was used in Functional Assay to report that bacterial T6SS5-dependent cell fusion triggers type I IFN gene expression in the host and leads to activation of the cGAMP synthase-stimulator of IFN genes (cGAS-STING) pathway, independent of bacterial ligands.

Tue Jul 07 00:00:00 EDT 2020

Gastrodin Inhibits Virus Infection by Promoting the Production of Type I Interferon.

Frontiers in pharmacology

Zhou Y,Li M,Lv T,Huang M,Cheng B,Zhang Y,Zhu J

Published figure using IFNAR1 monoclonal antibody (Product # 16-5945-85) in Neutralization

Tue Mar 30 00:00:00 EDT 2021

Type I Interferon Receptor Signaling in Astrocytes Regulates Hippocampal Synaptic Plasticity and Cognitive Function of the Healthy CNS.

Cell reports

Hosseini S,Michaelsen-Preusse K,Grigoryan G,Chhatbar C,Kalinke U,Korte M

16-5945-85 was used in Functional assay to demonstrate the role of type I interferon receptor on synaptic plasticity and cognitive function in mice.

Tue May 19 00:00:00 EDT 2020

PHA eludes macrophage suppression to activate CD8⁺ T cells.

Immunobiology

Lomakova YD,Londregan J,Maslanka J,Goldman N,Somerville J,Riggs JE

16-5945-85 was used in Enzyme-linked immunosorbent assay to demonstrate that C57BL/6 J peritoneal cell culture models features of macrophage-dense tumors as TCR ligation fails to activate T cells unless IFNγ is neutralized or iNOS is inhibited.

Tue Jan 01 00:00:00 EST 2019

Cell-type- and region-specific restriction of neurotropic flavivirus infection by viperin.

Journal of neuroinflammation

Lindqvist R,Kurhade C,Gilthorpe JD,Överby AK

16-5945 was used in Functional assays to show that viperin restricts replication of neurotropic flaviviruses in the central nervous system in a region- and cell-type-specific manner.

Thu Mar 15 00:00:00 EDT 2018

C-type lectin receptor DCIR modulates immunity to tuberculosis by sustaining type I interferon signaling in dendritic cells.

Proceedings of the National Academy of Sciences of the United States of America

Troegeler A,Mercier I,Cougoule C,Pietretti D,Colom A,Duval C,Vu Manh TP,Capilla F,Poincloux R,Pingris K,Nigou J,Rademann J,Dalod M,Verreck FA,Al Saati T,Lugo-Villarino G,Lepenies B,Hudrisier D,Neyrolles O

16-5945 was used in Flow cytometry/Cell sorting to reveal the modulatory role of the C-type lectin, DCIR, in tuberculosis-associated inflammation.

Tue Jan 24 00:00:00 EST 2017

C-type lectin receptor DCIR modulates immunity to tuberculosis by sustaining type I interferon signaling in dendritic cells.

Proceedings of the National Academy of Sciences of the United States of America

16-5945 was used in Flow cytometry/Cell sorting to reveal the modulatory role of the C-type lectin, DCIR, in tuberculosis-associated inflammation.

Tue Jan 24 00:00:00 EST 2017

Plasmacytoid dendritic cells orchestrate TLR7-mediated innate and adaptive immunity for the initiation of autoimmune inflammation.

Scientific reports

Takagi H,Arimura K,Uto T,Fukaya T,Nakamura T,Choijookhuu N,Hishikawa Y,Sato K

16-5945 was used in Functional assays to investigate the role of plasmacytoid dendritic cells in autoimmunity, showing that they orchestrate TLR7-mediated immune response and initiate autoimmune inflammation.

Thu Apr 14 00:00:00 EDT 2016

Fast type I interferon response protects astrocytes from flavivirus infection and virus-induced cytopathic effects.

Journal of neuroinflammation

Lindqvist R,Mundt F,Gilthorpe JD,Wölfel S,Gekara NO,Kröger A,Överby AK

16-5945 was used in Blocking experiments to show that astrocytes control viral replication of different TBEV strains, JEV, WNV, and ZIKV. In contrast to fibroblast, astrocytes mount a rapid interferon response and restrict viral spread.

Mon Oct 24 00:00:00 EDT 2016

TLR and RLR Signaling Are Reprogrammed in Opposite Directions after Detection of Viral Infection.

Journal of immunology (Baltimore, Md. : 1950)

Hotz C,Roetzer LC,Huber T,Sailer A,Oberson A,Treinies M,Heidegger S,Herbst T,Endres S,Bourquin C

16-5945 was used in an ELISA assay to demonstrate that innate recognition of viral infection critically modifies responses to pattern-recognition receptor stimulation.

Sun Nov 01 00:00:00 EDT 2015

Basophils regulate the recruitment of eosinophils in a murine model of irritant contact dermatitis.

The Journal of allergy and clinical immunology

Nakashima C,Otsuka A,Kitoh A,Honda T,Egawa G,Nakajima S,Nakamizo S,Arita M,Kubo M,Miyachi Y,Kabashima K

16-5945 was used in Flow cytometry/Cell sorting to clarify the roles of eosinophils in irritant contact dermatitis and the underlying mechanism of eosinophil infiltration of the skin.

Tue Jul 01 00:00:00 EDT 2014

SARM regulates CCL5 production in macrophages by promoting the recruitment of transcription factors and RNA polymerase II to the Ccl5 promoter.

Journal of immunology (Baltimore, Md. : 1950)

Gürtler C,Carty M,Kearney J,Schattgen SA,Ding A,Fitzgerald KA,Bowie AG

16-5945 was used in Neutralization experiments to characterise TLR-induced cytokine responses in murine macrophages deficient in the Toll/IL-1R adaptor sterile α and HEAT/Armadillo motif-containing protein (SARM).

Thu May 15 00:00:00 EDT 2014

A type I IFN-Flt3 ligand axis augments plasmacytoid dendritic cell development from common lymphoid progenitors.

The Journal of experimental medicine

Chen YL,Chen TT,Pai LM,Wesoly J,Bluyssen HA,Lee CK

16-5945 was used in Flow cytometry/Cell sorting to define a critical role for the FL/IFN-I/Flt3 axis in plasmacytoid dendritic cell differentiation from common lymphoid progenitors.

Mon Nov 18 00:00:00 EST 2013

Basophils support the survival of plasma cells in mice.

Journal of immunology (Baltimore, Md. : 1950)

Rodriguez Gomez M,Talke Y,Goebel N,Hermann F,Reich B,Mack M

16-5945 was used in Neutralization experiments to investigate whether basophils support plasma cell survival and Ig production.

Wed Dec 15 00:00:00 EST 2010

Bioinformatics

Protein Aliases: H3K1; IFN R1; IFN-alpha/beta receptor 1; IFN-alpha/betaR; IFN-alpha/betaR1; IFN-alpha/betaRalpha; IFN-R-1; IFNalpha/beta R1; IFNalpha/beta-Ralpha; IFNalpha/betaR1; IFNR1; INF-a receptor; Interferon alpha/beta receptor 1; interferon-alpha/beta receptor 1; Type I interferon receptor 1

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Gene Aliases: CD118; Ifar; Ifnar; Ifnar1; Ifrc; Infar

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UniProt ID: (Mouse) P33896

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Entrez Gene ID: (Mouse) 15975

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Function(s)

phosphoribosylamine-glycine ligase activity interferon receptor activity transmembrane signal receptor

Process(es)

cytokine-mediated signaling pathway positive regulation of interferon-gamma production T cell activation type I interferon biosynthetic process positive regulation of interleukin-1 beta secretion regulation of peptidyl-tyrosine phosphorylation defense response to virus

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IFNAR1 Monoclonal Antibody (MAR1-5A3), Functional Grade, eBioscience™

IFNAR1 Antibody (16-5945-85) in Neu

Product Details

16-5945-85

Flow Cytometry (Flow)

ELISA (ELISA)

Neutralization (Neu)

Functional Assay (Functional)

Inhibition Assays (IA)

Blocking Assay (BLOCK)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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