Invitrogen

CD19 Monoclonal Antibody (MB19-1), APC, eBioscience™

FIGURE: 1 / 1

CD19 Antibody (17-0191-82) in Flow

Staining of mouse splenocytes with Anti-Mouse CD3e FITC (Product # 11-0031-82) and staining buffer (autofluorescence) (left) or 0.25 µg of Anti-Mouse CD19 APC (right). Total viable cells were used for analysis.

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Product Details

17-0191-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.5 µg/test

View 15 publications 15 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Mouse / IgA, kappa

Class

Monoclonal

Type

Antibody

Clone

MB19-1

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_469358

Product Specific Information

Description: The MB19-1 monoclonal antibody reacts with mouse CD19, a 95 kDa transmembrane glycoprotein. CD19 is expressed by B cells during all stages of development excluding the terminally differentiated plasma cells. Follicular dendritic cells also express CD19. Together CD21, CD81, MHC class II, and CD19 form a multimolecular complex that associates with the BCR. Signaling through CD19 induces tyrosine phosphorylation, calcium flux and proliferation of B cells. Staining of B cells with MB19-1 and its conjugates is usually dimmer than the rat anti-mouse CD19 antibody, clone 6D5.

Applications Reported: The MB19-1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The MB19-1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD19 is a member of the immunoglobulin superfamily and has two Ig like domains. The CD19 molecule is expressed on 100% of the peripheral B cells as defined by expression of kappa or lambda light chains. CD19 appears to be expressed on myeloid leukemia cells, particularly those of monocytic lineage. Leukemia phenotype studies have demonstrated that the earliest and broadest B cell restricted antigen is the CD19 antigen. The receptor for CD19 is an important functional regulator of normal and malignant B cell proliferation, and is expressed in all B cell precursor leukemias. Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. CD19 is a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. Besides being a signal-amplifying coreceptor for the B cell receptor (BCR), CD19 can also signal independently of BCR co-ligation and is a central regulatory component upon which multiple signaling pathways converge. Mutation of the CD19 gene results in hypogammaglobulinemia, whereas CD19 overexpression causes B cell hyperactivity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Tnpo3 controls splicing of the pre-mRNA encoding the canonical TCR α chain of iNKT cells.

Nature communications

Iwanami N,Richter AS,Sikora K,Boehm T

17-0191-82 was used in Flow cytometry/Cell sorting to identify a role for Tnpo3 in regulating the splicing of the pre-mRNA encoding the cognate TCRα chain of iNKT cells.

Tue Jun 20 00:00:00 EDT 2023

Prophylactic Antiheparanase Activity by PG545 Is Antiviral In Vitro and Protects against Ross River Virus Disease in Mice.

Antimicrobial agents and chemotherapy

Supramaniam A,Liu X,Ferro V,Herrero LJ

17-0191 was used in Flow cytometry/Cell sorting to describe both prophylactic and therapeutic treatment with PG545, a low-molecular-weight heparan sulfate mimetic, for arthritogenic alphaviral infection.

Sun Apr 01 00:00:00 EDT 2018

Helminth-induced IL-4 expands bystander memory CD8⁺ T cells for early control of viral infection.

Nature communications

Rolot M,Dougall AM,Chetty A,Javaux J,Chen T,Xiao X,Machiels B,Selkirk ME,Maizels RM,Hokke C,Denis O,Brombacher F,Vanderplasschen A,Gillet L,Horsnell WGC,Dewals BG

17-0191 was used in Flow cytometry/Cell sorting to study whether helminth-induced type 2 immunity functionally affects the T virtual memory cell compartment.

Tue Oct 30 00:00:00 EDT 2018

G-CSF is a key modulator of MDSC and could be a potential therapeutic target in colitis-associated colorectal cancers.

Protein & cell

Li W,Zhang X,Chen Y,Xie Y,Liu J,Feng Q,Wang Y,Yuan W,Ma J

17-0191 was used in Flow cytometry/Cell sorting to indicate that G-CSF is a critical regulating molecule in the migration, proliferation and functional maintenance of myeloid derived suppressor cells.

Mon Feb 01 00:00:00 EST 2016

Expression of CD11c Is Associated with Unconventional Activated T Cell Subsets with High Migratory Potential.

PloS one

Qualai J,Li LX,Cantero J,Tarrats A,Fernández MA,Sumoy L,Rodolosse A,McSorley SJ,Genescà M

17-0191 was used in Flow cytometry/Cell sorting to find markers associated with non-conventional T cell subsets, including γδ T cells and invariant natural killer T cells.

Thu Mar 16 00:00:00 EDT 2017

Pentosan Polysulfate: a Novel Glycosaminoglycan-Like Molecule for Effective Treatment of Alphavirus-Induced Cartilage Destruction and Inflammatory Disease.

Journal of virology

Herrero LJ,Foo SS,Sheng KC,Chen W,Forwood MR,Bucala R,Mahalingam S

17-0191 was used in Flow cytometry/Cell sorting to demonstrate the potential of glycotherapeutics as a new class of treatment for infectious arthritis.

Sat Aug 01 00:00:00 EDT 2015

Depletion of foxp3(+) T cells abrogates tolerance of skin and heart allografts in murine mixed chimeras without the loss of mixed chimerism.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons

Shinoda K,Akiyoshi T,Chase CM,Farkash EA,Ndishabandi DK,Raczek CM,Sebastian DP,Pelle PD,Russell PS,Madsen JC,Colvin RB,Alessandrini A

17-0191 was used in Flow cytometry/Cell sorting to investigate the relative contribution of central and peripheral mechanisms to the generation of skin and heart allograft tolerance in murine mixed chimeras.

Wed Oct 01 00:00:00 EDT 2014

IL-7 produced by thymic epithelial cells plays a major role in the development of thymocytes and TCRγδ+ intraepithelial lymphocytes.

Journal of immunology (Baltimore, Md. : 1950)

Shitara S,Hara T,Liang B,Wagatsuma K,Zuklys S,Holländer GA,Nakase H,Chiba T,Tani-ichi S,Ikuta K

17-0191 was used in Flow cytometry/Cell sorting to suggest that TEC-derived IL-7 is important in proliferation, survival, and maturation of thymocytes and γδ T-cell development.

Sat Jun 15 00:00:00 EDT 2013

R-phycoerythrin-conjugated antibodies are inappropriate for intracellular staining of murine plasma cells.

Cytometry. Part A : the journal of the International Society for Analytical Cytology

Kim MS,Kim TS

17-0191-82 was used in Sample Preparation to show that PE-conjugated antibodies are inappropriate for intracellular staining of murine plasma cells.

Wed May 01 00:00:00 EDT 2013

Macrophage migration inhibitory factor receptor CD74 mediates alphavirus-induced arthritis and myositis in murine models of alphavirus infection.

Arthritis and rheumatism

Herrero LJ,Sheng KC,Jian P,Taylor A,Her Z,Herring BL,Chow A,Leo YS,Hickey MJ,Morand EF,Ng LF,Bucala R,Mahalingam S

17-0191 was used in Flow cytometry/Cell sorting to investigate the role that CD74 plays in the immunopathogenesis of arthritic alphavirus infection and myositis in mice.

Tue Oct 01 00:00:00 EDT 2013

Methotrexate treatment causes early onset of disease in a mouse model of Ross River virus-induced inflammatory disease through increased monocyte production.

PloS one

Taylor A,Sheng KC,Herrero LJ,Chen W,Rulli NE,Mahalingam S

17-0191 was used in Flow cytometry/Cell sorting to study infiltration of inflammatory cells to skeletal muscle in Ross River virus disease in response to MTX treatment.

Thu Mar 06 00:00:00 EST 2014

IKKα-mediated signaling circuitry regulates early B lymphopoiesis during hematopoiesis.

Blood

Balkhi MY,Willette-Brown J,Zhu F,Chen Z,Liu S,Guttridge DC,Karin M,Hu Y

17-0191-82 was used in Flow Cytometry to demonstrate that IKKα regulates B-lineage commitment via combined canonical and noncanonical NF-κB transcriptional activities to target Pax5 expression during hematopoiesis.

Thu Jun 07 00:00:00 EDT 2012

Dermatan sulfate interacts with dead cells and regulates CD5(+) B-cell fate: implications for a key role in autoimmunity.

The American journal of pathology

Wang JY,Lee J,Yan M,Rho JH,Roehrl MH

17-0191 was used in Flow cytometry/Cell sorting to investigate the mechanisms underlying the positive selection and expansion of CD5(+) B cells, showing that dermatan sulfate interacts with dead cells and regulates CD5(+) B-cell fate.

Sun May 01 00:00:00 EDT 2011

Intermediate-term hematopoietic stem cells with extended but time-limited reconstitution potential.

Cell stem cell

Benveniste P,Frelin C,Janmohamed S,Barbara M,Herrington R,Hyam D,Iscove NN

17-0191 was used in Flow cytometry/Cell sorting to investigate the presence of "intermediate-term" multipotent hematopoietic stem cells, revealing that these cells are able to sustain clones of differentiating cells for only 4-6 weeks.

Fri Jan 08 00:00:00 EST 2010

Evaluation of intranuclear BrdU detection procedures for use in multicolor flow cytometry.

Cytometry. Part A : the journal of the International Society for Analytical Cytology

Rothaeusler K,Baumgarth N

17-0191 was used in Flow cytometry/Cell sorting to assess the viability of a novel detection method.

Sat Apr 01 00:00:00 EST 2006

Bioinformatics

Protein Aliases: B-lymphocyte antigen CD19; CD19; Differentiation antigen CD19; Leu-12

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Gene Aliases: AW495831; Cd19

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UniProt ID: (Mouse) P25918

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Entrez Gene ID: (Mouse) 12478

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Process(es)

chromosome breakage B cell receptor signaling pathway positive regulation of release of sequestered calcium ion into cytosol

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD19 Monoclonal Antibody (MB19-1), APC, eBioscience™

CD19 Antibody (17-0191-82) in Flow

Product Details

17-0191-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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