Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
CD36 Monoclonal Antibody (HM36), APC, eBioscience™
Advanced Verification
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 4
CD36 Antibody (17-0362-82) in Flow
BALB/c mouse thioglycolate-elicited peritoneal exudate cells were stained with F4/80 Monoclonal Antibody, FITC (Product # 11-4801-82) and 0.25 µg of Armenian Hamster IgG Isotype Control, APC (Product # 17-4888-82) (left) or 0.25 µg of CD36 Monoclonal Antibody, APC (right).
Product Details
17-0362-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Armenian hamster
/ IgG
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
HM36
Immunogen
CHO cells transfected with full-length mouse CD36
Conjugate
Excitation/Emission Max
651/660 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2734967
Product Specific Information
Description: This HM36 antibody recognizes mouse CD36, also known as scavenger receptor BIII.
Applications Reported: This HM36 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This HM36 antibody has been tested by flow cytometric analysis of thioglycolate-elicited peritoneal macrophages. This may be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser
Target Information
CD36, also known as scavenger receptor class B member 3, is a protein that is expressed on the surface of various cell types, including macrophages, platelets, and adipocytes. It plays a role in lipid metabolism, inflammation, and atherosclerosis, and is involved in the recognition and uptake of various ligands such as oxidized low-density lipoproteins, long-chain fatty acids, and apoptotic cells. CD36 is also implicated in the pathogenesis of malaria. The protein encoded by this gene serves as a receptor for thrombospondin in platelets and various cell lines, and is the fourth major glycoprotein of the platelet surface. It binds to collagen, thrombospondin, anionic phospholipids, and oxidized LDL, and directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes. Mutations in this gene cause platelet glycoprotein deficiency. Multiple alternatively spliced transcript variants have been found for this gene. Diseases associated with CD36 include Platelet Glycoprotein IV Deficiency and Coronary Heart Disease 7.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
Bioinformatics
Protein Aliases: CD36; fatty acid translocase; Glycoprotein IIIb; GPIIIB; GPIV; PAS IV; PAS-4; Platelet glycoprotein 4; Platelet glycoprotein IV; SR-B3
Gene Aliases: Cd36; FAT; GPIV; Scarb3
UniProt ID: (Mouse) Q08857
Entrez Gene ID: (Mouse) 12491
low-density lipoprotein receptor activity
protein binding
high-density lipoprotein particle binding
lipid binding
low-density lipoprotein particle binding
transforming growth factor beta binding
thrombospondin receptor activity
oleic acid binding
lipoteichoic acid receptor activity
lipoprotein particle binding
membrane trafficking regulatory protein
negative regulation of transcription from RNA polymerase II promoter
long-chain fatty acid metabolic process
positive regulation of cell-matrix adhesion
pattern recognition receptor signaling pathway
negative regulation of systemic arterial blood pressure
fatty acid metabolic process
triglyceride metabolic process
transport
receptor-mediated endocytosis
phagocytosis, recognition
cell adhesion
signal transduction
cell surface receptor signaling pathway
nitric oxide mediated signal transduction
response to mechanical stimulus
negative regulation of gene expression
positive regulation of macrophage derived foam cell differentiation
positive regulation of cholesterol storage
fatty acid transport
long-chain fatty acid transport
plasma membrane long-chain fatty acid transport
negative regulation of angiogenesis
fatty acid oxidation
lipid storage
cGMP-mediated signaling
positive regulation of blood coagulation
cholesterol transport
positive regulation of interleukin-12 production
positive regulation of interleukin-6 production
positive regulation of tumor necrosis factor production
plasma lipoprotein particle clearance
low-density lipoprotein particle clearance
lipoprotein transport
negative regulation of transcription factor import into nucleus
positive regulation of I-kappaB kinase/NF-kappaB signaling
apoptotic cell clearance
positive regulation of MAPK cascade
regulation of transcription from RNA polymerase II promoter in response to oxidative stress
negative regulation of growth of symbiont in host
long-chain fatty acid import
positive regulation of cytokine secretion
positive regulation of peptidyl-tyrosine phosphorylation
defense response to Gram-positive bacterium
low-density lipoprotein particle mediated signaling
positive regulation of phagocytosis, engulfment
positive regulation of macrophage cytokine production
cellular response to bacterial lipopeptide
cellular response to lipopolysaccharide
cellular response to lipoteichoic acid
cellular response to hydroperoxide
regulation of removal of superoxide radicals
positive regulation of blood microparticle formation
positive regulation of reactive oxygen species metabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support