Invitrogen

CD43 Monoclonal Antibody (eBio84-3C1 (84-3C1)), APC, eBioscience™

FIGURE: 1 / 15

CD43 Antibody (17-0439-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD3 FITC (Product # 11-0038-42) and Mouse IgG1 kappa Isotype Control APC (Product # 17-4714-81) (left) or Anti-Human CD43 APC (right). Cells in the lymphocyte gate were used for analysis.

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Product Details

17-0439-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.25 µg)/test

View 9 publications 9 publications

Product Specifications

Species Reactivity

Human

Published species

Fish, Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBio84-3C1 (84-3C1)

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11219080

Product Specific Information

Description: The monoclonal antibody ebio84-3C1 reacts with CD43, which is also known as leukosialin, galactoglycoprotein and sialophorin. CD43 is a sialomucin which like many mucins can have both adhesive and anti-adhesive functions. Expression of CD43 is found on most leukocytes except resting B lymphocytes. Proteolytic processing upon activation decreases surface expression. CD43 is involved in activation of T cells, B cells, NK cells, and monocytes. The counter-receptor for CD43 is CD169/SIGLEC-1, which is expressed on macrophages.

Applications Reported: This eBio84-3C1 (84-3C1) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBio84-3C1 (84-3C1) antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood monocytes. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD43 (leukosialin, sialophorin) is a transmembrane mucin-like protein with high negative charge, expressed on the surface of most hematopoietic cells. CD43 contributes to a repulsive barrier that interferes with cellular adhesion, however, in certain cases also promotes leukocyte aggregation. By interaction with actin-binding proteins ezrin and moesin CD43 plays a regulatory role in remodeling T-cell morphology and regulates cell-cell interactions during lymphocyte traffic. CD43 signaling both enhances LFA-1 adhesiveness and counteracts LFA-1 induction via other receptors. Expression of CD43 causes induction of functionally active tumor suppressor p53 protein, but in case of p53 and ARF deficiency CD43 promotes tumor proliferation and viability. CD43 appears to be an important modulator of leukocyte functions. Diseases associated with CD43 dysfunction include Wiscott-Aldrich Syndrome and Adenoid Basal Cell Carcinoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

In vitro differentiation of human embryonic stem cells to hemogenic endothelium and blood progenitors via embryoid body formation.

STAR protocols

Garcia-Alegria E,Potts B,Menegatti S,Kouskoff V

Published figure using CD43 monoclonal antibody (Product # 17-0439-42) in Flow Cytometry

Fri Mar 19 00:00:00 EDT 2021

Verification and rectification of cell type-specific splicing of a Seckel syndrome-associated ATR mutation using iPS cell model.

Journal of human genetics

Ichisima J,Suzuki NM,Samata B,Awaya T,Takahashi J,Hagiwara M,Nakahata T,Saito MK

Published figure using CD43 monoclonal antibody (Product # 17-0439-42) in Flow Cytometry

Wed May 01 00:00:00 EDT 2019

The Generation of Human γδT Cell-Derived Induced Pluripotent Stem Cells from Whole Peripheral Blood Mononuclear Cell Culture.

Stem cells translational medicine

Watanabe D,Koyanagi-Aoi M,Taniguchi-Ikeda M,Yoshida Y,Azuma T,Aoi T

Published figure using CD43 monoclonal antibody (Product # 17-0439-42) in Flow Cytometry

Mon Jan 01 00:00:00 EST 2018

Activation of KLF1 Enhances the Differentiation and Maturation of Red Blood Cells from Human Pluripotent Stem Cells.

Stem cells (Dayton, Ohio)

Yang CT,Ma R,Axton RA,Jackson M,Taylor AH,Fidanza A,Marenah L,Frayne J,Mountford JC,Forrester LM

17-0439 was used in Flow cytometry/Cell sorting to investigate the role of KLF1 in maturation of red blood cells.

Sat Apr 01 00:00:00 EDT 2017

Aberrant expression of interleukin-10 and activation-induced cytidine deaminase in B cells from patients with Behçet's disease.

Biomedical reports

Yoon JY,Lee Y,Yu SL,Yoon HK,Park HY,Joung CI,Park SR,Kwon M,Kang J

Published figure using CD43 monoclonal antibody (Product # 17-0439-42) in Flow Cytometry

Fri Dec 01 00:00:00 EST 2017

Enforced Expression of HOXB4 in Human Embryonic Stem Cells Enhances the Production of Hematopoietic Progenitors but Has No Effect on the Maturation of Red Blood Cells.

Stem cells translational medicine

Jackson M,Ma R,Taylor AH,Axton RA,Easterbrook J,Kydonaki M,Olivier E,Marenah L,Stanley EG,Elefanty AG,Mountford JC,Forrester LM

Published figure using CD43 monoclonal antibody (Product # 17-0439-42) in Flow Cytometry

Mon Aug 01 00:00:00 EDT 2016

Mitochondrial ATP transporter Ant2 depletion impairs erythropoiesis and B lymphopoiesis.

Cell death and differentiation

Cho J,Seo J,Lim CH,Yang L,Shiratsuchi T,Lee MH,Chowdhury RR,Kasahara H,Kim JS,Oh SP,Lee YJ,Terada N

Published figure using CD43 monoclonal antibody (Product # 17-0439-42) in Flow Cytometry

Tue Sep 01 00:00:00 EDT 2015

Rapid Proliferation and Differentiation of a Subset of Circulating IgM Memory B Cells to a CpG/Cytokine Stimulus In Vitro.

PloS one

Vásquez C,Franco MA,Angel J

17-0439 was used in Flow cytometry/Cell sorting to compare the phenotype and in vitro functional response of various immune cells in response to CpG and a cytokine cocktail.

Thu Jun 02 00:00:00 EDT 2016

Reprogramming human endothelial cells to haematopoietic cells requires vascular induction.

Nature

Sandler VM,Lis R,Liu Y,Kedem A,James D,Elemento O,Butler JM,Scandura JM,Rafii S

17-0439 was used in Flow cytometry/Cell sorting to investigate the role of inductive cues from the vascular niche in coordinating and sustaining haematopoietic specification.

Thu Jul 17 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: CD43; Galactoglycoprotein; GALGP; GPL115; Leukocyte sialoglycoprotein; Leukosialin; Sialophorin; sialophorin (gpL115, leukosialin, CD43); sialophorin gpL115

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Gene Aliases: CD43; GALGP; GPL115; LSN; SPN

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UniProt ID: (Human) P16150

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Entrez Gene ID: (Human) 6693

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Function(s)

transmembrane signaling receptor activity

Process(es)

response to protozoan negative regulation of type IV hypersensitivity positive regulation of protein phosphorylation chemotaxis immune response cellular defense response negative regulation of cell adhesion establishment or maintenance of cell polarity signal transduction cell surface receptor signaling pathway T cell costimulation positive regulation of T cell proliferation negative regulation of T cell proliferation positive regulation of tumor necrosis factor biosynthetic process defense response to bacterium negative thymic T cell selection regulation of defense response to virus leukocyte migration apoptotic signaling pathway

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD43 Monoclonal Antibody (eBio84-3C1 (84-3C1)), APC, eBioscience™

CD43 Antibody (17-0439-42) in Flow

Product Details

17-0439-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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