Invitrogen

CD47 Monoclonal Antibody (B6H12), APC, eBioscience™

15 Published Figures

14 References

View all (156) CD47 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (15)

FIGURE: 1 / 16

CD47 Antibody (17-0479-42) in Flow

Staining of normal human peripheral blood cells with Mouse IgG1 kappa Isotype Control APC (Product # 17-4714-81) (blue histogram) or Anti-Human CD47 APC (purple histogram). Cells in the lymphocyte gate were used for analysis.

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Product Details

17-0479-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (1 µg)/test

View 13 publications 13 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

B6H12

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1963586

Product Specific Information

Description: The monoclonal antibody B6H12 reacts to CD47 also known as integrin-associated protein (IAP), and neurophilin. CD47 is a glycosylated five transmembrane protein with a small alternatively spliced cytoplasmic domain. CD47 is involved in adhesion through interactions with SIRP (signal regulator protein) and is non-covalently associated with beta3 integrins CD51/CD61 and CD41/CD61. Furthermore this interaction can mediate bi-directional signaling to modify neural synaptic activity and regulate the phagocytic activities of macrophages. CD47 is the receptor for thrombospondin. T cell expression of CD47 can mediate activation or apoptosis (in the presence of high levels of thrombospondin). Recently stimulation of CD47 by monoclonal antibody has been shown to induce CD4+CD25- suppressive activity also increasing expression of Foxp3. Expression is found in the majority of hematopoietic cells including T and B cells, monocytes, platelets and erythrocytes (as part of the Rh complex). Expression is also found in non-hematopoietic cells.

This antibody has been reported to have neutralizing activity.

Applications Reported: This B6H12 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This B6H12 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD47 (integrin-associated protein, IAP) is a ubiquitously expressed cell surface transmembrane glycoprotein interacting with several integrins and regulating their functions. Engagement of CD47 by soluble ligands or counter receptors modulates various signaling pathways, such as activation of heterotrimeric G proteins. Functionally, CD47 is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. CD47 is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. CD47 has broad tissue distribution, is reduced in expression on Rh erythrocytes. CD47 acts as a ligand for CD172a (signal regulatory protein alpha, SIRP alpha), an immune inhibitory receptor on macrophages; this interaction prevents phagocytosis of CD47-positive cells. Moreover, CD47-CD172a system affects cell migration, B cell adhesion, T cell activation, and is involved in modulation of chondrocyte responses to mechanical signals, and promotes neuronal development, being especially abundant in synapse-rich regions of brain and retina.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Altered Membrane Expression and Function of CD11b Play a Role in the Immunosuppressive Effects of Morphine on Macrophages at the Nanomolar Level.

Pharmaceuticals (Basel, Switzerland)

Yu PC,Hao CY,Fan YZ,Liu D,Qiao YF,Yao JB,Li CZ,Yu Y

17-0479-42 was used in Flow cytometry/Cell sorting to find that morphine at 0.1-10 nM levels inhibited CD11b expression and function on macrophages via a µ-opioid receptor (MOR)-dependent mechanism, thereby reducing macrophage phagocytosis of tumor cells, a process that can be reversed by thymopentin (TP5), a commonly used immune-enhancing adjuvant in clinical practice.

Mon Feb 13 00:00:00 EST 2023

Altered Membrane Expression and Function of CD11b Play a Role in the Immunosuppressive Effects of Morphine on Macrophages at the Nanomolar Level.

Pharmaceuticals (Basel, Switzerland)

Yu PC,Hao CY,Fan YZ,Liu D,Qiao YF,Yao JB,Li CZ,Yu Y

Mon Feb 13 00:00:00 EST 2023

Schweinfurthin induces ICD without ER stress and caspase activation.

Oncoimmunology

Zhang R,Neighbors JD,Schell TD,Hohl RJ

Published figure using CD47 monoclonal antibody (Product # 17-0479-42) in Flow Cytometry

Tue Aug 09 00:00:00 EDT 2022

Co-delivery of phagocytosis checkpoint and STING agonist by a Trojan horse nanocapsule for orthotopic glioma immunotherapy.

Theranostics

Zhou Y,Guo Y,Chen L,Zhang X,Wu W,Yang Z,Li X,Wang Y,Hu Z,Wang Z

17-0479-42 was used in Preparation of nanocapsules to develop a smart "Trojan horse" BBB-permeable nanocapsule termed "NAcp@CD47" to deliver anti-CD47 antibodies and stimulator of interferon genes (STING) agonists into GBM tissues in a stealth-like manner to reshaped the immune microenvironment by switching the phenotype of microglia and macrophages.

Tue Aug 02 00:00:00 EDT 2022

Anti-CD47 antibody synergizes with cisplatin against laryngeal cancer by enhancing phagocytic ability of macrophages.

Clinical and experimental immunology

Wang J,Zhang H,Yin X,Bian Y

Published figure using CD47 monoclonal antibody (Product # 17-0479-42) in Flow Cytometry

Wed Sep 01 00:00:00 EDT 2021

LSD1 silencing contributes to enhanced efficacy of anti-CD47/PD-L1 immunotherapy in cervical cancer.

Cell death & disease

Xu S,Wang X,Yang Y,Li Y,Wu S

17-0479-42 was used in Flow Cytometry to validate the increase in expression of LSD1 during the progression of cervical cancer and confirmed the enhanced therapeutic effect against cervical cancer when combining CD47/PD-L1 blockade therapy with an LSD1 inhibitor.

Wed Mar 17 00:00:00 EDT 2021

LSD1 silencing contributes to enhanced efficacy of anti-CD47/PD-L1 immunotherapy in cervical cancer.

Cell death & disease

Xu S,Wang X,Yang Y,Li Y,Wu S

Wed Mar 17 00:00:00 EDT 2021

Exosome-mediated delivery of CRISPR/Cas9 for targeting of oncogenic Kras^G12D in pancreatic cancer.

Life science alliance

McAndrews KM,Xiao F,Chronopoulos A,LeBleu VS,Kugeratski FG,Kalluri R

Published figure using CD47 monoclonal antibody (Product # 17-0479-42) in Flow Cytometry

Wed Sep 01 00:00:00 EDT 2021

Regulation of senescence escape by TSP1 and CD47 following chemotherapy treatment.

Cell death & disease

Guillon J,Petit C,Moreau M,Toutain B,Henry C,Roché H,Bonichon-Lamichhane N,Salmon JP,Lemonnier J,Campone M,Verrièle V,Lelièvre E,Guette C,Coqueret O

17-0479-42 was used in Flow Cytometry to characterise the emergence of transformed tumour cells following chemotherapy-mediated senescence, and demonstrate that senescent cells favour tumour growth and metastasis, in vitro and in vivo.

Wed Feb 27 00:00:00 EST 2019

Regulation of senescence escape by TSP1 and CD47 following chemotherapy treatment.

Cell death & disease

Guillon J,Petit C,Moreau M,Toutain B,Henry C,Roché H,Bonichon-Lamichhane N,Salmon JP,Lemonnier J,Campone M,Verrièle V,Lelièvre E,Guette C,Coqueret O

Wed Feb 27 00:00:00 EST 2019

Generation and testing of clinical-grade exosomes for pancreatic cancer.

JCI insight

Mendt M,Kamerkar S,Sugimoto H,McAndrews KM,Wu CC,Gagea M,Yang S,Blanko EVR,Peng Q,Ma X,Marszalek JR,Maitra A,Yee C,Rezvani K,Shpall E,LeBleu VS,Kalluri R

Published figure using CD47 monoclonal antibody (Product # 17-0479-42) in Flow Cytometry

Thu Apr 19 00:00:00 EDT 2018

Programmed cell removal by calreticulin in tissue homeostasis and cancer.

Nature communications

Feng M,Marjon KD,Zhu F,Weissman-Tsukamoto R,Levett A,Sullivan K,Kao KS,Markovic M,Bump PA,Jackson HM,Choi TS,Chen J,Banuelos AM,Liu J,Gip P,Cheng L,Wang D,Weissman IL

17-0479 was used in Flow cytometry/Cell sorting to show that unwanted cells such as ageing neutrophils and living cancer cells are susceptible to "labelling" by secreted calreticulin from macrophages, enabling their clearance through programmed cell removal.

Fri Aug 10 00:00:00 EDT 2018

Programmed cell removal by calreticulin in tissue homeostasis and cancer.

Nature communications

Feng M,Marjon KD,Zhu F,Weissman-Tsukamoto R,Levett A,Sullivan K,Kao KS,Markovic M,Bump PA,Jackson HM,Choi TS,Chen J,Banuelos AM,Liu J,Gip P,Cheng L,Wang D,Weissman IL

Fri Aug 10 00:00:00 EDT 2018

CD47 promotes ovarian cancer progression by inhibiting macrophage phagocytosis.

Oncotarget

Liu R,Wei H,Gao P,Yu H,Wang K,Fu Z,Ju B,Zhao M,Dong S,Li Z,He Y,Huang Y,Yao Z

Published figure using CD47 monoclonal antibody (Product # 17-0479-42) in Flow Cytometry

Tue Jun 13 00:00:00 EDT 2017

Anti-SIRPα antibodies as a potential new tool for cancer immunotherapy.

JCI insight

Yanagita T,Murata Y,Tanaka D,Motegi SI,Arai E,Daniwijaya EW,Hazama D,Washio K,Saito Y,Kotani T,Ohnishi H,Oldenborg PA,Garcia NV,Miyasaka M,Ishikawa O,Kanai Y,Komori T,Matozaki T

17-0479 was used in Functional assays to show that SIRPα is highly expressed in human renal cell carcinoma and melanoma, and that anti-SIRPα antibody blocks the interaction with CD47, suppressing tumor formation by renal cell carcinoma or melanoma cells.

Thu Jan 12 00:00:00 EST 2017

A CD47-associated super-enhancer links pro-inflammatory signalling to CD47 upregulation in breast cancer.

Nature communications

Betancur PA,Abraham BJ,Yiu YY,Willingham SB,Khameneh F,Zarnegar M,Kuo AH,McKenna K,Kojima Y,Leeper NJ,Ho P,Gip P,Swigut T,Sherwood RI,Clarke MF,Somlo G,Young RA,Weissman IL

17-0479 was used in Flow cytometry/Cell sorting to suggest that cancers can evolve SE to drive CD47 overexpression to escape immune surveillance.

Wed Apr 05 00:00:00 EDT 2017

Evolution of acute myelogenous leukemia stem cell properties after treatment and progression.

Blood

Ho TC,LaMere M,Stevens BM,Ashton JM,Myers JR,O'Dwyer KM,Liesveld JL,Mendler JH,Guzman M,Morrissette JD,Zhao J,Wang ES,Wetzler M,Jordan CT,Becker MW

17-0479 was used in Flow cytometry/Cell sorting to provide a new frame of reference by which to evaluate candidate AML therapies in which both disease control and the induction of more advanced forms of disease should be considered.

Thu Sep 29 00:00:00 EDT 2016

Cells expressing the C/EBPbeta isoform, LIP, engulf their neighbors.

PloS one

Abreu M,Sealy L

17-0479 was used in Flow cytometry/Cell sorting to provide evidence that the transcription factor, LIP, induces cells to ingest other cells.

Thu Apr 11 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: antigen identified by monoclonal antibody 1D8; Antigenic surface determinant protein OA3; CD47; CD47 antigen; CD47 antigen (Rh-related antigen, integrin-associated signal transducer); CD47 glycoprotein; IAP; integrin associated protein; Integrin-associated protein; integrin-associated signal transducer; Leukocyte surface antigen CD47; Protein MER6; Rh-related antigen; sCD47; soluble CD 47; soluble CD47

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Gene Aliases: CD47; IAP; MER6; OA3

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UniProt ID: (Human) Q08722

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Entrez Gene ID: (Human) 961

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Function(s)

protein binding thrombospondin receptor activity cell adhesion molecule

Process(es)

cell adhesion integrin-mediated signaling pathway opsonization positive regulation of cell proliferation response to bacterium positive regulation of cell-cell adhesion extracellular matrix organization positive regulation of inflammatory response positive regulation of phagocytosis positive regulation of T cell activation leukocyte migration

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CD47 Monoclonal Antibody (B6H12), APC, eBioscience™

CD47 Antibody (17-0479-42) in Flow

Product Details

17-0479-42

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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