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Invitrogen
CD147 Monoclonal Antibody (8D12), APC, eBioscience™
Product Details
17-1472-42
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
8D12
Conjugate
Excitation/Emission Max
651/660 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with 0.2% BSA
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_10714990
Product Specific Information
Description: The monoclonal antibody 8D12 recognizes CD147 which is a member of the immunoglobulin superfamily. It is also called EMMPRIN and basigin. CD147 has been characterized as a tumor cell collagenase stimulating molecule and has also been shown to have a role in cell adhesion. CD147 can be found in association with integrins alpha3 beta1 and alpha6 beta1 by co-immpunopreciptations. Additionally it is thought to play a role in T cell activation and thymocyte cycling. CD147 is expressed broadly on hematopoietic and non-hematopoietic cells, especially in tumor cells.
Applications Reported: This 8D12 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 8D12 antibody has been pre-titrated and tested by flow cytometric analysis or normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD147 (basigin, neurothelin, OX-47, 5A11, CE9, M6, EMMPRIN, extracellular matrix metalloproteinase inducer, TCSF, tumour cell-derived collagenase-stimulatory factor) is a 50-60 kDa glycoprotein with multiple glycosylated forms. CD147 is a plasma membrane protein that is important in spermatogenesis, embryo implantation, neural network formation, and tumor progression. CD147 is also a member of the immunoglobulin superfamily. The highest level of CD147 expression is on metabolically active cells, such as lymphoblasts, inflammatory cells, brown adipocytes and malignant tumor cells. CD147 has multiple functions, including facilitating of cell surface expression of monocarboxylate transporter proteins and extracellular matrix metalloproteinases, regulation of integrin functions, it plays roles in cell development and activation, fetal development or retinal function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 5F7; Basigin; CD147; Collagenase stimulatory factor; EMMPRIN; Extracellular matrix metalloproteinase inducer; HAb18G; Hepatoma-associated antigen; Leukocyte activation antigen M6; OK blood group antigen; TCSF; Tumor cell-derived collagenase stimulatory factor
Gene Aliases: 5F7; BSG; CD147; EMMPRIN; OK; TCSF; UNQ6505/PRO21383
UniProt ID: (Human) P35613
Entrez Gene ID: (Human) 682
pyruvate metabolic process
cell surface receptor signaling pathway
embryo implantation
monocarboxylic acid transport
extracellular matrix disassembly
extracellular matrix organization
odontogenesis of dentin-containing tooth
response to peptide hormone
response to mercury ion
decidualization
leukocyte migration
response to cAMP
protein targeting to plasma membrane
cell-cell adhesion
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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