Invitrogen

CD223 (LAG-3) Monoclonal Antibody (3DS223H), APC, eBioscience™

CD223 (LAG-3) Antibody in Flow Cytometry (Flow)

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CD223 (LAG-3) Antibody (17-2239-42) in Flow

Normal human peripheral blood cells were stimulated for 3 days with Human IL-2 Recombinant Protein (Product # 14-8029-81), Anti-Human CD3, and Anti-Human CD28 Functional Grade Purifieds (Product # 16-0037-81 and Product # 16-0289-81). These cells were then surface stained with Anti-Human CD8a FITC (Product # 11-0087-42) and Mouse IgG1 K Isotype Control APC (Product # 17-4714-81) (left) or Anti-Human CD223 (LAG-3) APC (right). Viable cells, as determined by Fixable Viability Dye eFluor® 450 (Product # 65-0863-14), in the lymphocyte gate wer... View More

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Product Details

17-2239-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.06 µg)/test

View 17 publications 17 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Rhesus monkey

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

3DS223H

Immunogen

hCD223-FC PFA 4%

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2573186

Product Specific Information

Description: This 3DS223H monoclonal antibody recognizes human CD223 also known as Lymphocyte Activation Gene 3 (LAG-3). LAG-3 is a 70-kDa surface glycoprotein belonging to the Ig superfamily with homology to CD4. LAG-3 binds to MHC class II with higher affinity than CD4 and is thought to be involved in the negative regulation of T cell activation and homeostatic proliferation. Surface expression of LAG-3 has been reported on activated T cells (including regulatory T cells) and NK cells. CD8+ T cells usually express LAG-3 at significantly higher levels than CD4+ T cells. Coexpression of LAG-3 and CD49b has been proposed to identify human and mouse Type 1 regulatory T cells (Tr1 cells).

This 3DS223H antibody will recognize a formaldehyde-fixed epitope.

Applications Reported: This 3DS223H antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 3DS223H antibody has been pre-titrated and tested by flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

LAG-3 is a 70-kDa surface glycoprotein belonging to the Ig superfamily with homology to CD4. LAG-3 binds to MHC class II with higher affinity than CD4 and is thought to be involved in the negative regulation of T cell activation and homeostatic proliferation. Surface expression of LAG-3 has been reported on activated T cells (including regulatory T cells) and NK cells. CD8+ T cells usually express LAG-3 at significantly higher levels than CD4+ T cells. Coexpression of LAG-3 and CD49b has been proposed to identify human and mouse Type 1 regulatory T cells (Tr1 cells).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Low-density lipoprotein balances T cell metabolism and enhances response to anti-PD-1 blockade in a HCT116 spheroid model.

Frontiers in oncology

Babl N,Hofbauer J,Matos C,Voll F,Menevse AN,Rechenmacher M,Mair R,Beckhove P,Herr W,Siska PJ,Renner K,Kreutz M,Schnell A

Published figure using CD223 (LAG-3) monoclonal antibody (Product # 17-2239-42) in Flow Cytometry

Tue Feb 14 00:00:00 EST 2023

Proteogenomic analysis reveals RNA as a source for tumor-agnostic neoantigen identification.

Nature communications

Tretter C,de Andrade Krätzig N,Pecoraro M,Lange S,Seifert P,von Frankenberg C,Untch J,Zuleger G,Wilhelm M,Zolg DP,Dreyer FS,Bräunlein E,Engleitner T,Uhrig S,Boxberg M,Steiger K,Slotta-Huspenina J,Ochsenreither S,von Bubnoff N,Bauer S,Boerries M,Jost PJ,Schenck K,Dresing I,Bassermann F,Friess H,Reim D,Grützmann K,Pfütze K,Klink B,Schröck E,Haller B,Kuster B,Mann M,Weichert W,Fröhling S,Rad R,Hiltensperger M,Krackhardt AM

17-2239-42 was used in Flow cytometry/Cell sorting to underline the importance of RNA-centered variant detection for the identification of shared biomarkers and potentially relevant neoantigen candidates.

Wed Aug 02 00:00:00 EDT 2023

Bispecific T-Cell Engaging Antibodies Against MUC16 Demonstrate Efficacy Against Ovarian Cancer in Monotherapy and in Combination With PD-1 and VEGF Inhibition.

Frontiers in immunology

Yeku OO,Rao TD,Laster I,Kononenko A,Purdon TJ,Wang P,Cui Z,Liu H,Brentjens RJ,Spriggs D

17-2239-42 was used in Flow Cytometry to show the feasibility and efficacy of human MUC16 ectodomain- specific BiTEDs and provide a basis for the combination with anti-VEGF therapy for ovarian cancer.

Thu Oct 21 00:00:00 EDT 2021

Bispecific T-Cell Engaging Antibodies Against MUC16 Demonstrate Efficacy Against Ovarian Cancer in Monotherapy and in Combination With PD-1 and VEGF Inhibition.

Frontiers in immunology

Yeku OO,Rao TD,Laster I,Kononenko A,Purdon TJ,Wang P,Cui Z,Liu H,Brentjens RJ,Spriggs D

Thu Oct 21 00:00:00 EDT 2021

Increased Intrahepatic Expression of Immune Checkpoint Molecules in Autoimmune Liver Disease.

Cells

Macek Jilkova Z,Hilleret MN,Gerster T,Sturm N,Mercey-Ressejac M,Zarski JP,Leroy V,Marche PN,Costentin C,Decaens T

17-2239-42 was used in Flow Cytometry to show that the ICM play an important role in the loss of immune homeostasis in the liver, providing an attractive approach to investigate their role as targets for effective therapeutic interventions.

Wed Oct 06 00:00:00 EDT 2021

PD-1 Blockade Reinvigorates Bone Marrow CD8⁺ T Cells from Patients with Multiple Myeloma in the Presence of TGFβ Inhibitors.

Clinical cancer research : an official journal of the American Association for Cancer Research

Kwon M,Kim CG,Lee H,Cho H,Kim Y,Lee EC,Choi SJ,Park J,Seo IH,Bogen B,Song IC,Jo DY,Kim JS,Park SH,Choi I,Choi YS,Shin EC

17-2239 was used in Flow cytometry/Cell sorting to indicate that combined blockade of PD-1 and TGFβ may be useful for the treatment of multiple myeloma.

Wed Apr 01 00:00:00 EDT 2020

MAVS Genetic Variation Is Associated with Decreased HIV-1 Replication In Vitro and Reduced CD4⁺ T Cell Infection in HIV-1-Infected Individuals.

Viruses

Stunnenberg M,van Pul L,Sprokholt JK,van Dort KA,Gringhuis SI,Geijtenbeek TBH,Kootstra NA

Published figure using CD223 (LAG-3) monoclonal antibody (Product # 17-2239-42) in Flow Cytometry

Thu Jul 16 00:00:00 EDT 2020

Tumor response and endogenous immune reactivity after administration of HER2 CAR T cells in a child with metastatic rhabdomyosarcoma.

Nature communications

Hegde M,Joseph SK,Pashankar F,DeRenzo C,Sanber K,Navai S,Byrd TT,Hicks J,Xu ML,Gerken C,Kalra M,Robertson C,Zhang H,Shree A,Mehta B,Dakhova O,Salsman VS,Grilley B,Gee A,Dotti G,Heslop HE,Brenner MK,Wels WS,Gottschalk S,Ahmed N

Published figure using CD223 (LAG-3) monoclonal antibody (Product # 17-2239-42) in Flow Cytometry

Wed Jul 15 00:00:00 EDT 2020

Symmetric Arginine Dimethylation Is Selectively Required for mRNA Splicing and the Initiation of Type I and Type III Interferon Signaling.

Cell reports

Metz PJ,Ching KA,Xie T,Delgado Cuenca P,Niessen S,Tatlock JH,Jensen-Pergakes K,Murray BW

17-2239 was used in Flow cytometry/Cell sorting to uncover a precise set of mRNA transcripts that require symmetric arginine dimethylation.

Tue Feb 11 00:00:00 EST 2020

Systematically optimized BCMA/CS1 bispecific CAR-T cells robustly control heterogeneous multiple myeloma.

Nature communications

Zah E,Nam E,Bhuvan V,Tran U,Ji BY,Gosliner SB,Wang X,Brown CE,Chen YY

17-2239 was used in Flow cytometry/Cell sorting to demonstrate that BCMA/CS1 bispecific CAR-T cells exhibit superior CAR expression and function compared to T cells that co-express individual BCMA and CS1 CARs.

Fri May 08 00:00:00 EDT 2020

Divergent LAG-3 versus BTLA, TIGIT, and FCRL3 expression in Sézary syndrome.

Leukemia & lymphoma

Anzengruber F,Ignatova D,Schlaepfer T,Chang YT,French LE,Pascolo S,Contassot E,Bobrowicz M,Hoetzenecker W,Guenova E

Published figure using CD223 (LAG-3) monoclonal antibody (Product # 17-2239-42) in Flow Cytometry

Thu Aug 01 00:00:00 EDT 2019

Primary T Cells from Cutaneous T-cell Lymphoma Skin Explants Display an Exhausted Immune Checkpoint Profile.

Cancer immunology research

Querfeld C,Leung S,Myskowski PL,Curran SA,Goldman DA,Heller G,Wu X,Kil SH,Sharma S,Finn KJ,Horwitz S,Moskowitz A,Mehrara B,Rosen ST,Halpern AC,Young JW

17-2239 was used in Flow cytometry/Cell sorting to identify the antigens driving T-cell exhaustion and the evaluation of immune checkpoint inhibition to reverse T-cell exhaustion earlier in treatment of cutaneous T-cell lymphoma.

Wed Aug 01 00:00:00 EDT 2018

Differential expression pattern of co-inhibitory molecules on CD4⁺ T cells in uncomplicated versus complicated malaria.

Scientific reports

Abel A,Steeg C,Aminkiah F,Addai-Mensah O,Addo M,Gagliani N,Casar C,Yar DD,Owusu-Dabo E,Jacobs T,Mackroth MS

17-2239 was used in Flow cytometry/Cell sorting to identify CD4+ T cell signatures that are associated with protection from severe and/or clinical malaria.

Mon Mar 19 00:00:00 EDT 2018

Low-dose interleukin-2 promotes STAT-5 phosphorylation, T_reg survival and CTLA-4-dependent function in autoimmune liver diseases.

Clinical and experimental immunology

Jeffery HC,Jeffery LE,Lutz P,Corrigan M,Webb GJ,Hirschfield GM,Adams DH,Oo YH

17-2239 was used in Flow cytometry/Cell sorting to establish the potential of interleukin-2 to enhance regulatory T cell therapy in autoimmune liver disease.

Thu Jun 01 00:00:00 EDT 2017

OMIP-037: 16-color panel to measure inhibitory receptor signatures from multiple human immune cell subsets.

Cytometry. Part A : the journal of the International Society for Analytical Cytology

Belkina AC,Snyder-Cappione JE

Published figure using CD223 (LAG-3) monoclonal antibody (Product # 17-2239-42) in Flow Cytometry

Wed Feb 01 00:00:00 EST 2017

Regulatory T Cell Modulation by CBP/EP300 Bromodomain Inhibition.

The Journal of biological chemistry

Ghosh S,Taylor A,Chin M,Huang HR,Conery AR,Mertz JA,Salmeron A,Dakle PJ,Mele D,Cote A,Jayaram H,Setser JW,Poy F,Hatzivassiliou G,DeAlmeida-Nagata D,Sandy P,Hatton C,Romero FA,Chiang E,Reimer T,Crawford T,Pardo E,Watson VG,Tsui V,Cochran AG,Zawadzke L,Harmange JC,Audia JE,Bryant BM,Cummings RT,Magnuson SR,Grogan JL,Bellon SF,Albrecht BK,Sims RJ,Lora JM

17-2239 was used in Flow cytometry/Cell sorting to demonstrate a critical role for CREBBP/EP300 bromodomains in regulatory T cell biology.

Fri Jun 17 00:00:00 EDT 2016

Progression of Lung Cancer Is Associated with Increased Dysfunction of T Cells Defined by Coexpression of Multiple Inhibitory Receptors.

Cancer immunology research

Thommen DS,Schreiner J,Müller P,Herzig P,Roller A,Belousov A,Umana P,Pisa P,Klein C,Bacac M,Fischer OS,Moersig W,Savic Prince S,Levitsky V,Karanikas V,Lardinois D,Zippelius A

17-2239 was used in Flow cytometry/Cell sorting to characterise the diversity and expression patterns of inhibitory receptors on tumour-infiltrating T cells from patients with non-small cell lung cancer.

Tue Dec 01 00:00:00 EST 2015

Follicular regulatory T cells impair follicular T helper cells in HIV and SIV infection.

Nature communications

Miles B,Miller SM,Folkvord JM,Kimball A,Chamanian M,Meditz AL,Arends T,McCarter MD,Levy DN,Rakasz EG,Skinner PJ,Connick E

17-2239 was used in Flow cytometry/Cell sorting to determine TFR contribute to inefficient germinal centre responses and inhibit HIV and SIV clearance.

Tue Oct 20 00:00:00 EDT 2015

Follicular regulatory T cells impair follicular T helper cells in HIV and SIV infection.

Nature communications

Miles B,Miller SM,Folkvord JM,Kimball A,Chamanian M,Meditz AL,Arends T,McCarter MD,Levy DN,Rakasz EG,Skinner PJ,Connick E

17-2239 was used in Flow cytometry/Cell sorting to determine TFR contribute to inefficient germinal centre responses and inhibit HIV and SIV clearance.

Tue Oct 20 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: CD223; LAG-3; Lymphocyte activation gene 3 protein; lymphocyte-activation gene 3; sLAG 3; sLAG3; soluble LAG 3lymphocyte activating 3; soluble LAG3

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Gene Aliases: CD223; FDC; LAG3

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UniProt ID: (Human) P18627

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Entrez Gene ID: (Human) 3902

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Function(s)

antigen binding transmembrane signaling receptor activity MHC class II protein binding transmembrane signal receptor

Process(es)

cell surface receptor signaling pathway negative regulation of interleukin-2 biosynthetic process positive regulation of natural killer cell mediated cytotoxicity negative regulation of T cell activation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD223 (LAG-3) Monoclonal Antibody (3DS223H), APC, eBioscience™

CD223 (LAG-3) Antibody (17-2239-42) in Flow

Product Details

17-2239-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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