Invitrogen

BCL6 Monoclonal Antibody (BCL-DWN), APC, eBioscience™

1 Reference
View all (100) BCL6 antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

17-5453-82

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

0.03 µg/test
View 1 publication 1 publication
Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), APC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

BCL-DWN

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2573214

Product Specific Information

Description: This BCL-DWN monoclonal antibody reacts with mouse Bcl-6, which is also known as B-cell lymphoma 6. A member of the POZ family of transcriptional repressors, Bcl-6 is expressed most abundantly in germinal center B cells, as well as in differentiating and activated B cells. This 79-kDa protein is essential for germinal center B cell proliferation and survival. Moreover, during B cell development, Bcl-6 plays an important role in promoting pre-B cell survival to enable the generation of a diverse B cell repertoire. Moreover, Bcl-6 is expressed constitutively in diffuse large B cell lymphomas (DLBCLs), Burkitt's lymphoma, and non-Hodgkin's lymphoma. Reports also suggest expression of Bcl-6 in CD4+ T follicular helper cells and memory T cells. Bcl-6 has been reported to be antagonized by Blimp-1.

Applications Reported: This BCL-DWN antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This BCL-DWN antibody has been tested by intracellular staining followed by flow cytometric analysis of splenocytes isolated from sheep red blood cell-immunized mice using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Please refer to Best Protocols: Protocol B: One step protocol for (nuclear) intracellular proteins located under the Resources Tab online. This can be used at less than or equal to 0.03 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Bcl-6 proto-oncogene product (a Kruppel-type zinc-finger protein) is mainly expressed in normal germinal center B cells and related lymphomas. Bcl-6 is involved in chromosome rearrangements at 3q27 in non-Hodgkin's lymphomas and Bcl-6 rearrangements have been detected in 33%-45% of diffuse large B cell lymphomas. Bcl-6 has been detected immunohistochemically in follicular lymphomas, diffuse large B cell lymphomas, Burkitt's lymphomas and in nodular, lymphocyte predominant Hodgkin's disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Bioinformatics

Protein Aliases: B-cell leukemia/lymphoma 5; B-cell leukemia/lymphoma 6; B-cell lymphoma 5 protein (BCL5); B-cell lymphoma 6 protein homolog; cys his2 zinc finger transcription factor; LAZ-3 protein; Lymphoma Associated Zinc Finger Gene On Chromosome 3 (LAZ3); Zinc finger and BTB domain-containing protein 27 (ZBTB27); Zinc Finger Protein 51 (ZNF51)

View more View less

Gene Aliases: Bcl-6; Bcl5; Bcl6

View more View less

UniProt ID: (Mouse) P41183

View more View less

Entrez Gene ID: (Mouse) 12053

View more View less

Function(s)
RNA polymerase II regulatory region sequence-specific DNA binding intronic transcription regulatory region sequence-specific DNA binding transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding nucleic acid binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding protein binding chromatin DNA binding sequence-specific DNA binding metal ion binding C2H2 zinc finger transcription factor
Process(es)
protein import into nucleus, translocation negative regulation of transcription from RNA polymerase II promoter cell morphogenesis negative regulation of cell-matrix adhesion immune system process germinal center formation negative regulation of type 2 immune response transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter inflammatory response cellular response to DNA damage stimulus Rho protein signal transduction spermatogenesis protein localization negative regulation of cell proliferation actin cytoskeleton organization B cell differentiation negative regulation of cell growth positive regulation of B cell proliferation positive regulation of histone deacetylation negative regulation of mast cell cytokine production negative regulation of Rho protein signal transduction type 2 immune response regulation of cell proliferation positive regulation of apoptotic process negative regulation of apoptotic process regulation of GTPase activity regulation of memory T cell differentiation negative regulation of cell differentiation negative regulation of T-helper 2 cell differentiation positive regulation of neuron differentiation negative regulation of Notch signaling pathway negative regulation of transcription, DNA-templated negative regulation of isotype switching to IgE isotypes erythrocyte development regulation of inflammatory response positive regulation of cellular component movement negative regulation of cellular senescence
guarantee_icon

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more
help_icon

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact tech support