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Invitrogen
TCR V delta 1 Monoclonal Antibody (TS8.2), APC, eBioscience™
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 2
TCR V delta 1 Antibody (17-5679-42) in Flow
Normal human peripheral blood cells were stained with CD3 Monoclonal Antibody, PE (Product # 12-0038-42), TCR gamma-delta Monoclonal Antibody, PerCP-eFluor 710 (Product # 46-9959-42) and either Mouse IgG1 kappa Isotype Control, APC (Product # 17-4714-42) (left) or TCR V delta 1 Monoclonal Antibody, APC (right). Viable CD3 positive lymphocytes were used for analysis.
Product Details
17-5679-42
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Not Applicable
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
TS8.2
Immunogen
Full length protein of Human TCR V delta 1
Conjugate
Excitation/Emission Max
651/660 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with 0.2% BSA
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2762442
Product Specific Information
Description: This TS8.2 monoclonal antibody recognizes human TCR V delta 1, a common framework determinant on the delta-subunit of the T cell receptor. Gamma-delta T cells are primarily found within the epithelium. They show less TCR diversity and recognize antigens differently than alpha-beta T cells. Subsets of gamma-delta T cells have shown antitumor and immunoregulatory activity. TCR V delta 1 Monoclonal Antibody (TS8.2) has been validated for the following applications: Flow Cytometry
Applications Reported: This TS8.2 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This TS8.2 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser
Target Information
The T Cell Receptor gamma-delta (TCR gamma delta) is a heterodimeric membrane receptor that consists of the highly variable gamma and delta chains expressed as part of a complex with the invariant CD3 proteins. Gamma-delta T cells are less frequent than alpha-beta T cells but compose a substantial part of T cells found at epithelial borders including the gut mucosa resident intraepithelial lymphocytes (IEL). The antigenic stimuli required for gamma-delta T cell receptor activation are not well understood and are thought to be mainly MHC class-independent.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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