Invitrogen

GM-CSF Monoclonal Antibody (MP1-22E9), APC, eBioscience™

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Antibody Testing Data (1)
Published Figures (2)

GM-CSF Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 3

GM-CSF Antibody (17-7331-82) in Flow

eBioscience™ Mouse Cytokine Positive Control Cells (Product # 00-4500-51) were stained intracellularly, using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol, with CD4 Monoclonal Antibody, FITC (Product # 11-0042-82) and 0.25 µg of Rat IgG2a kappa Isotype Control, APC (Product # 17-4321-81) (left) or 0.25 µg of GM-CSF Monoclonal Antibody, APC (right). Cells in the lymphocyte gate were used for analysis.

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Product Details

17-7331-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.5 µg/test

View 7 publications 7 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), APC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

MP1-22E9

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2762700

Product Specific Information

Description: The MP1-22E9 antibody reacts with mouse granulocyte/macrophage - colony stimulating factor (GM-CSF). The MP1-22E9 antibody is a neutralizing antibody. Mouse GM-CSF is a 14 kDa factor produced mainly by activated T cells and macrophages. Other cell types, such as endothelium and fibroblasts, also secrete GM-CSF in response to TNF-alpha, IL-2, IL-1, and IFN-gamma. GM-CSF stimulates growth of macrophages, granulocytes and dendritic cells. GM-CSF is found as a membrane-bound form and also as a complex associated with the extracellular matrix. Non-glycosylated GM-CSF is biologically active.

Applications Reported: This MP1-22E9 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This MP1-22E9 antibody has been tested by intracellular staining followed by flow cytometric analysis of restimulated mouse splenocytes. This may be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser

Target Information

The GM-CSF gene encodes for a cytokine that regulates the production, differentiation, and function of macrophages and granulocytes. The active form of the protein exists as a homodimer in the extracellular space. This gene is located in a cluster of related genes at the chromosome region 5q31, which has been associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include interleukins 4, 5, and 13. The gene is involved in promoting tissue inflammation. Elevated levels of cytokines, including the one produced by this gene, have been observed in SARS-CoV-2 infected patients with acute respiratory distress syndrome. Mice that lack this gene or its receptor have been shown to develop pulmonary alveolar proteinosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Itaconate ameliorates autoimmunity by modulating T cell imbalance via metabolic and epigenetic reprogramming.

Nature communications

Aso K,Kono M,Kanda M,Kudo Y,Sakiyama K,Hisada R,Karino K,Ueda Y,Nakazawa D,Fujieda Y,Kato M,Amengual O,Atsumi T

17-7331-82 was used in Flow cytometry/Cell sorting to indicate that itaconate is a crucial metabolic regulator for Th17/Treg cell balance and could be a potential therapeutic agent for autoimmune diseases.

Mon Feb 27 00:00:00 EST 2023

Itaconate ameliorates autoimmunity by modulating T cell imbalance via metabolic and epigenetic reprogramming.

Nature communications

Aso K,Kono M,Kanda M,Kudo Y,Sakiyama K,Hisada R,Karino K,Ueda Y,Nakazawa D,Fujieda Y,Kato M,Amengual O,Atsumi T

Mon Feb 27 00:00:00 EST 2023

PD-L1 positive astrocytes attenuate inflammatory functions of PD-1 positive microglia in models of autoimmune neuroinflammation.

Nature communications

Linnerbauer M,Beyer T,Nirschl L,Farrenkopf D,Lößlein L,Vandrey O,Peter A,Tsaktanis T,Kebir H,Laplaud D,Oellinger R,Engleitner T,Alvarez JI,Rad R,Korn T,Hemmer B,Quintana FJ,Rothhammer V

17-7331-82 was used in Flow cytometry/Cell sorting to suggest the glial PD-L1/PD-1 axis as a potential therapeutic target for both acute and progressive MS stages.

Sat Sep 09 00:00:00 EDT 2023

Lysosomal acid lipase, CSF1R, and PD-L1 determine functions of CD11c+ myeloid-derived suppressor cells.

JCI insight

Zhao T,Liu S,Ding X,Johnson EM,Hanna NH,Singh K,Sen CK,Wan J,Du H,Yan C

17-7331-82 was used in Flow cytometry/Cell sorting to investigate if CD11c+ cells switched their functions to immune suppression and tumor growth stimulation through CSF1R/PD-L1 upregulation and metabolic reprogramming.

Thu Sep 08 00:00:00 EDT 2022

Cold exposure protects from neuroinflammation through immunologic reprogramming.

Cell metabolism

Spiljar M,Steinbach K,Rigo D,Suárez-Zamorano N,Wagner I,Hadadi N,Vincenti I,Page N,Klimek B,Rochat MA,Kreutzfeldt M,Chevalier C,Stojanović O,Bejuy O,Colin D,Mack M,Cansever D,Greter M,Merkler D,Trajkovski M

Published figure using GM-CSF monoclonal antibody (Product # 17-7331-82) in Flow Cytometry

Tue Nov 02 00:00:00 EDT 2021

Prevention of EAE by tolerogenic vaccination with PEGylated antigenic peptides.

Therapeutic advances in chronic disease

Pfeil J,Simonetti M,Lauer U,von Thülen B,Durek P,Poulsen C,Pawlowska J,Kröger M,Krähmer R,Leenders F,Hoffmann U,Hamann A

Published figure using GM-CSF monoclonal antibody (Product # 17-7331-82) in Flow Cytometry

Tue Nov 07 00:00:00 EST 2023

Astrocyte-Derived Pleiotrophin Mitigates Late-Stage Autoimmune CNS Inflammation.

Frontiers in immunology

Linnerbauer M,Lößlein L,Farrenkopf D,Vandrey O,Tsaktanis T,Naumann U,Rothhammer V

17-7331-82 was used in Flow Cytometry to demonstrate in vivo that astrocyte-derived pleiotrophin is critical for the recovery phase of autoimmune encephalomyelitis and limits chronic CNS inflammation.

Mon Feb 14 00:00:00 EST 2022

Astrocyte-Derived Pleiotrophin Mitigates Late-Stage Autoimmune CNS Inflammation.

Frontiers in immunology

Linnerbauer M,Lößlein L,Farrenkopf D,Vandrey O,Tsaktanis T,Naumann U,Rothhammer V

Mon Feb 14 00:00:00 EST 2022

Regulatory T Cells Restrain Interleukin-2- and Blimp-1-Dependent Acquisition of Cytotoxic Function by CD4⁺ T Cells.

Immunity

Śledzińska A,Vila de Mucha M,Bergerhoff K,Hotblack A,Demane DF,Ghorani E,Akarca AU,Marzolini MAV,Solomon I,Vargas FA,Pule M,Ono M,Seddon B,Kassiotis G,Ariyan CE,Korn T,Marafioti T,Lord GM,Stauss H,Jenner RG,Peggs KS,Quezada SA

Published figure using GM-CSF monoclonal antibody (Product # 17-7331-82) in Flow Cytometry

Tue Jan 14 00:00:00 EST 2020

Bioinformatics

Protein Aliases: Colony-stimulating factor; CSF; GM-CSF; granulocyte macrophage colony stimulating factor; granulocyte-macrophage colony stimulating factor 2; Granulocyte-macrophage colony-stimulating factor; M-GM-CSF; MGC131935; MGC138897; MGC151255; MGC151257; put. GM-CSF

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Gene Aliases: Csf2; Csfgm; Gm-CSf; GMCSF; MGI-IGM

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UniProt ID: (Mouse) P01587

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Entrez Gene ID: (Mouse) 12981

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Function(s)

cytokine activity granulocyte macrophage colony-stimulating factor receptor binding growth factor activity cytokine

Process(es)

embryonic placenta development immune response positive regulation of cell proliferation regulation of gene expression positive regulation of gene expression positive regulation of macrophage derived foam cell differentiation positive regulation of interleukin-23 production epithelial fluid transport macrophage activation regulation of cell proliferation positive regulation of tyrosine phosphorylation of Stat5 protein myeloid dendritic cell differentiation positive regulation of DNA replication negative regulation of cytolysis positive regulation of podosome assembly dendritic cell differentiation negative regulation of extrinsic apoptotic signaling pathway in absence of ligand

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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GM-CSF Monoclonal Antibody (MP1-22E9), APC, eBioscience™

GM-CSF Antibody (17-7331-82) in Flow

Product Details

17-7331-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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