Invitrogen

ID2 Monoclonal Antibody (ILCID2), APC, eBioscience™

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

3 Published Figures

3 References

View all (21) ID2 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

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FIGURE: 1 / 6

ID2 Antibody (17-9475-82) in Flow

C57BL/6 mouse splenocytes were stained intracellularly, using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol, with either 1.0 µg of Mouse IgG1 kappa Isotype Control, APC (Product # 17-4714-82) (left) or 1.0 µg of ID2 Monoclonal Antibody, APC (right). Cells were co-stained and gated based on the expression of both NK1.1 Monoclonal Antibody, eFluor 450 (Product # 48-5941-82) and CD49b Monoclonal Antibody, eFluor 450 (Product # 48-5971-82) (purple histogram); CD8a Monoclonal Antibody, PE-Cyanine 7 (Prod... View More

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ID2 Antibody — Intracellular staining of human peripheral blood cells. As expected based on known relative expression patterns, ID2 clone ILCID2 stains CD56+ lymphocytes but not CD56- lymphocytes. Details: Human PBMC were surface stained with CD56 (clone TULY56) and CD3 (clone UCHT1), followed by intracellular staining with ID2 (clone ILCID2) using the Foxp3/Transcription Factor Staining Buffer Set and protocol. Lymphocytes in the CD56+ (purple histogram), CD3+CD56- (orange histogram), and CD3-CD56- (blue histogram) gates were used for analysis. {RE}

Product Details

17-9475-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

1.0 µg/test

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Mouse

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

ILCID2

Immunogen

Purified recombinant fragment of human ID2, corresponding to amino acids 1-134 expressed in E. coli

View immunogen

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2735051

Product Specific Information

Description: This ILCID2 monoclonal antibody recognizes mouse and human ID2. This ILCID2 antibody requires Foxp3/Transcription Factor Staining Buffer set and will perform poorly when used with standard IC Fixation & Permeabilization Buffer Set and protocol.

Applications Reported: This ILCID2 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This ILCID2 antibody has been tested by flow cytometric analysis of mouse splenocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Please refer to Best Protocols: Protocol B: One step protocol for (nuclear) intracellular proteins located under the Resources Tab online. This may be used at less than or equal to 1.0 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Target Information

The inhibitor of DNA binding 2 (ID2) protein is essential for promoting the development of a number of cell types including natural killer cell, erythroid precursors, as well as, CD8+ and CD4+ T-cell. A lack of ID2 promotes B-cell development. The chief role of this protein is to associate with ubiquitously-expressed E proteins, and other proteins of the basic helix-loop-helix (HLH) family, preventing them from binding to DNA and activating transcription. Activation of ID2 is critical for the development of the natural killer cell lineage.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Progesterone attenuates Th17-cell pathogenicity in autoimmune uveitis via Id2/Pim1 axis.

Journal of neuroinflammation

Liu X,Gu C,Lv J,Jiang Q,Ding W,Huang Z,Liu Y,Su Y,Zhang C,Xu Z,Wang X,Su W

Published figure using ID2 monoclonal antibody (Product # 17-9475-82) in Flow Cytometry

Wed Jun 21 00:00:00 EDT 2023

Insulin-like Growth Factor 1 Supports a Pulmonary Niche that Promotes Type 3 Innate Lymphoid Cell Development in Newborn Lungs.

Immunity

Oherle K,Acker E,Bonfield M,Wang T,Gray J,Lang I,Bridges J,Lewkowich I,Xu Y,Ahlfeld S,Zacharias W,Alenghat T,Deshmukh H

17-9475 was used in Flow cytometry/Cell sorting to demonstrate that pulmonary type 3 innate lymphoid cells descended from innate lymphoid cell precursors that populated a niche defined by fibroblasts in the developing lung.

Tue Feb 18 00:00:00 EST 2020

Insulin-like Growth Factor 1 Supports a Pulmonary Niche that Promotes Type 3 Innate Lymphoid Cell Development in Newborn Lungs.

Immunity

Oherle K,Acker E,Bonfield M,Wang T,Gray J,Lang I,Bridges J,Lewkowich I,Xu Y,Ahlfeld S,Zacharias W,Alenghat T,Deshmukh H

Tue Feb 18 00:00:00 EST 2020

Transcriptome dynamics of CD4⁺ T cells during malaria maps gradual transit from effector to memory.

Nature immunology

Soon MSF,Lee HJ,Engel JA,Straube J,Thomas BS,Pernold CPS,Clarke LS,Laohamonthonkul P,Haldar RN,Williams CG,Lansink LIM,Moreira ML,Bramhall M,Koufariotis LT,Wood S,Chen X,James KR,Lönnberg T,Lane SW,Belz GT,Engwerda CR,Khoury DS,Davenport MP,Svensson V,Teichmann SA,Haque A

17-9475 was used in Flow cytometry/Cell sorting to apply single-cell RNA sequencing and computational modelling to track memory development during Plasmodium infection and treatment.

Tue Dec 01 00:00:00 EST 2020

Bioinformatics

Protein Aliases: bHLHb26; cell growth-inhibiting gene 8; Class B basic helix-loop-helix protein 26; DNA-binding protein inhibitor ID-2; DNA-binding protein inhibitor ID2; helix-loop-helix protein ID2; Inhibitor of differentiation 2; Inhibitor of DNA binding 2; inhibitor of DNA binding 2, dominant negative helix-loop-helix protein; MGC26389; OTTHUMP00000140258; OTTHUMP00000200297

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Gene Aliases: AI255428; BHLHB26; C78922; GIG8; Id-2; ID2; ID2A; ID2H; Idb2

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UniProt ID: (Human) Q02363, (Mouse) P41136

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Entrez Gene ID: (Human) 3398, (Mouse) 15902

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Function(s)

protein binding ion channel binding protein dimerization activity DNA-binding transcription factor

Process(es)

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ID2 Monoclonal Antibody (ILCID2), APC, eBioscience™

ID2 Antibody (17-9475-82) in Flow

Product Details

17-9475-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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