Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 1
AKT2 Antibody (200-508-J35) in DB
Dot Blot of AKT2 Phycoerythrin Conjugated Monoclonal Antibody. Antigen: AKT2 non-phosphorylated. Load: Lane 1: 50 ng. Lane 2: 16.67 ng. Lane 3: 5.56 ng. Lane 4: 1.85 ng. Lane 5: 0.62 ng. Primary antibody: none. Secondary antibody: AKT2 Phycoerythrin Conjugated Monoclonal Antibody at 1:1,000 in MB-070 for 60 min at RT. Block: MB-070 for 60 min at RT.
Product Details
200-508-J35
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rat
/ IgG2a, kappa
Class
Monoclonal
Type
Antibody
Clone
11F6.B2
Immunogen
Anti-AKT2 Antibody was produced in rat by repeated immunizations with a synthetic peptide corresponding to internal residues of human AKT2 protein.
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Lyophilized
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.5M NaCl, 10mg/mL BSA
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent). This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. Do not freeze after reconstitution. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.
Anti-AKT2 antibody is directed against human AKT2. The antibody detects both unphosphorylated and phosphorylated forms of the protein. Cross reactivity with AKT2 from other species has not been determined, however, the sequence of the immunogen shows 88% identity to mouse and 90% identity with rat, therefore, cross reactivity is expected.
Target Information
AKT2 is a phosphoinositide-dependent serine/threonine protein kinase that is enriched in insulin responsive tissues and has been implicated in the metabolic actions of the hormone. Akt is involved in many cellular signaling pathways and acts as a transducer of many functions initiated by growth factor receptors that activate phosphatidylinositol 3-kinase (PI 3-kinase). Akt2 is amplified and overexpressed in some human cancers.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: murine thymoma viral (v-akt) homolog-2; PKB beta; Protein kinase Akt-2; Protein kinase B beta; putative v-akt murine thymoma viral oncoprotein 2; RAC protein kinase beta; RAC-beta serine/threonine-protein kinase; RAC-PK-beta; v-akt murine thymoma viral oncogene homolog 2
Gene Aliases: AKT2; HIHGHH; PKBB; PKBBETA; PRKBB; RAC-BETA
UniProt ID: (Human) Q0VAN1
Entrez Gene ID: (Human) 208
positive regulation of protein phosphorylation
glycogen biosynthetic process
glucose metabolic process
regulation of translation
cellular protein modification process
apoptotic process
response to osmotic stress
signal transduction
insulin receptor signaling pathway
carbohydrate transport
positive regulation of signal transduction
negative regulation of plasma membrane long-chain fatty acid transport
positive regulation of glucose metabolic process
response to muscle activity
peptidyl-serine phosphorylation
regulation of cell migration
positive regulation of cell migration
positive regulation of vesicle fusion
response to nutrient levels
positive regulation of fatty acid beta-oxidation
peripheral nervous system myelin maintenance
cellular response to insulin stimulus
negative regulation of RNA splicing
positive regulation of peptidyl-serine phosphorylation
intracellular signal transduction
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
protein kinase B signaling
positive regulation of nitric oxide biosynthetic process
fat cell differentiation
positive regulation of glycogen biosynthetic process
positive regulation of transcription from RNA polymerase II promoter
positive regulation of glucose import
positive regulation of positive chemotaxis
mammary gland epithelial cell differentiation
intracellular protein transmembrane transport
regulation of cell cycle arrest
cellular response to organic cyclic compound
protein localization to plasma membrane
positive regulation of protein targeting to membrane
activation of GTPase activity
positive regulation of cell motility
positive regulation of glucose import in response to insulin stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support