Invitrogen

CD73 Monoclonal Antibody (AD2), PE-Cyanine7, eBioscience™

FIGURE: 1 / 40

CD73 Antibody (25-0739-42) in Flow

Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control PE-Cyanine7 (Product # 25-4714-80) (blue histogram) or Anti-Human CD73 PE-Cyanine7 (purple histogram). Cells in the lymphocyte gate were used for analysis.

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Product Details

25-0739-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.25 µg)/test

View 37 publications 37 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-Cyanine7, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

AD2

Conjugate

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

Excitation/Emission Max

569/780 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2573368

Product Specific Information

Description: This AD2 monoclonal antibody reacts with human CD73, a 5'-ectonucleotidase that converts 5'-adenosine monophosphate to adenosine. CD73 is expressed on the surface of endothelial cells, as well as B and T cells, including some CD4+Foxp3+ regulatory T cells. Adenosine production by these cells has been linked to the inhibition of CD4 T cell effector functions such as proliferation and cytokine secretion.

Applications Reported: This AD2 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This AD2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD73 (Ecto-5-prime-nucleotidase, 5-prime-ribonucleotide phosphohydrolase) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. CD73 consists of a dimer of 2 identical 70 kDa subunits bound externally to the plasma membrane by a glycosyl phosphatidyl inositol linkage. CD73 is used as a marker of lymphocyte differentiation. Consequently, a deficiency of CD73 occurs in a variety of immunodeficiency diseases. Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from CD73 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate. The CD73 gene has been localized to 6q14-q21 by immunofluorescence and a study of a panel of human x mouse hybrids that contained fragments of chromosome 6 as translocations. Defects in the CD73 gene can lead to the calcification of joints and arteries, and intestinal tuberculosis. Two transcript variants encoding different isoforms of CD73 have been found.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

The matrix microenvironment influences but does not dominate tissue-specific stem cell lineage differentiation.

Materials today. Bio

Pei YA,Mikaeiliagah E,Wang B,Zhang X,Pei M

Published figure using CD73 monoclonal antibody (Product # 25-0739-42) in Flow Cytometry

Fri Dec 01 00:00:00 EST 2023

Mesenchymal stem cells, as glioma exosomal immunosuppressive signal multipliers, enhance MDSCs immunosuppressive activity through the miR-21/SP1/DNMT1 positive feedback loop.

Journal of nanobiotechnology

Qiu W,Guo Q,Guo X,Wang C,Li B,Qi Y,Wang S,Zhao R,Han X,Du H,Zhao S,Pan Z,Fan Y,Wang Q,Gao Z,Li G,Xue H

Published figure using CD73 monoclonal antibody (Product # 25-0739-42) in Flow Cytometry

Sat Jul 22 00:00:00 EDT 2023

Exosomal CD73 from serum of patients with melanoma suppresses lymphocyte functions and is associated with therapy resistance to anti-PD-1 agents.

Journal for immunotherapy of cancer

Turiello R,Capone M,Morretta E,Monti MC,Madonna G,Azzaro R,Del Gaudio P,Simeone E,Sorrentino A,Ascierto PA,Morello S

Published figure using CD73 monoclonal antibody (Product # 25-0739-42) in Flow Cytometry

Tue Mar 01 00:00:00 EST 2022

Induced Neurodifferentiation of hBM-MSCs through Activation of the ERK/CREB Pathway via Pulsed Electromagnetic Fields and Physical Stimulation Promotes Neurogenesis in Cerebral Ischemic Models.

International journal of molecular sciences

Park HJ,Choi JH,Nam MH,Seo YK

Published figure using CD73 monoclonal antibody (Product # 25-0739-42) in Flow Cytometry

Fri Jan 21 00:00:00 EST 2022

Reprogramming bone progenitor identity and potency through control of collagen density and oxygen tension.

iScience

Al Hosni R,Bozec L,Roberts SJ,Cheema U

Published figure using CD73 monoclonal antibody (Product # 25-0739-42) in Flow Cytometry

Fri Apr 15 00:00:00 EDT 2022

Human placental mesenchymal stem cells regulate inflammation via the NF‑κB signaling pathway.

Experimental and therapeutic medicine

Liu Y,Zhang X,Hu Y,Kang M,Wu Y,Wang Y,Deng C

Published figure using CD73 monoclonal antibody (Product # 25-0739-42) in Flow Cytometry

Tue Nov 01 00:00:00 EDT 2022

hUCMSCs carrying exenatide prevent T1DM by improving intestinal microflora composition and islet tissue damage repair.

Molecular medicine (Cambridge, Mass.)

Wang W,Wang Y,Chi J,Tan X,Hu J,Ma X,Sun X,Che K,Lv W,Wang Y

Published figure using CD73 monoclonal antibody (Product # 25-0739-42) in Flow Cytometry

Tue Dec 13 00:00:00 EST 2022

VAP-PLGA microspheres (VAP-PLGA) promote adipose-derived stem cells (ADSCs)-induced wound healing in chronic skin ulcers in mice via PI3K/Akt/HIF-1α pathway.

Bioengineered

Jiang W,Zhang J,Zhang X,Fan C,Huang J

Published figure using CD73 monoclonal antibody (Product # 25-0739-42) in Flow Cytometry

Wed Dec 01 00:00:00 EST 2021

Peptide 11R‑VIVIT promotes fracture healing in osteoporotic rats.

International journal of molecular medicine

Hou C,Wang X,Jiang W,Bian Z,Zhu L,Li M

Published figure using CD73 monoclonal antibody (Product # 25-0739-42) in Flow Cytometry

Sun Aug 01 00:00:00 EDT 2021

Mesenchymal stem cells negatively regulate CD4⁺ T cell activation in patients with primary Sjögren syndrome through the miRNA‑125b and miRNA‑155 TCR pathway.

Molecular medicine reports

Gong B,Zheng L,Lu Z,Huang J,Pu J,Pan S,Zhang M,Liu J,Tang J

25-0739 was used in Flow cytometry/Cell sorting to indicate that the expression levels of miRNA‚125b‚5p and miRNA‚155 in TCR‚activated CD4+ T cells from patients with pSS may provide insight regarding autoimmune diseases and offer a novel target for prospective treatment.

Fri Jan 01 00:00:00 EST 2021

Bcl-xL mutant promotes cartilage differentiation of BMSCs by upregulating TGF-β/BMP expression levels.

Experimental and therapeutic medicine

Xiao K,Yang L,Xie W,Gao X,Huang R,Xie M

Published figure using CD73 monoclonal antibody (Product # 25-0739-42) in Flow Cytometry

Thu Jul 01 00:00:00 EDT 2021

TRPA1 triggers hyperalgesia and inflammation after tooth bleaching.

Scientific reports

Chen C,Huang X,Zhu W,Ding C,Huang P,Li R

Published figure using CD73 monoclonal antibody (Product # 25-0739-42) in Flow Cytometry

Tue Aug 31 00:00:00 EDT 2021

In Vitro Anti-cancer Activity of Adipose-Derived Mesenchymal Stem Cells Increased after Infection with Oncolytic Reovirus.

Advanced pharmaceutical bulletin

Babaei A,Bannazadeh Baghi H,Nezhadi A,Jamalpoor Z

Published figure using CD73 monoclonal antibody (Product # 25-0739-42) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2021

In vitro differentiation of human embryonic stem cells to hemogenic endothelium and blood progenitors via embryoid body formation.

STAR protocols

Garcia-Alegria E,Potts B,Menegatti S,Kouskoff V

Published figure using CD73 monoclonal antibody (Product # 25-0739-42) in Flow Cytometry

Fri Mar 19 00:00:00 EDT 2021

Therapeutic potential of human umbilical cord mesenchymal stem cells on aortic atherosclerotic plaque in a high-fat diet rabbit model.

Stem cell research & therapy

Li Y,Shi G,Han Y,Shang H,Li H,Liang W,Zhao W,Bai L,Qin C

Published figure using CD73 monoclonal antibody (Product # 25-0739-42) in Flow Cytometry

Thu Jul 15 00:00:00 EDT 2021

Down-Regulated Exosomal MicroRNA-221 - 3p Derived From Senescent Mesenchymal Stem Cells Impairs Heart Repair.

Frontiers in cell and developmental biology

Sun L,Zhu W,Zhao P,Zhang J,Lu Y,Zhu Y,Zhao W,Liu Y,Chen Q,Zhang F

Published figure using CD73 monoclonal antibody (Product # 25-0739-42) in Flow Cytometry

Mon Sep 28 00:00:00 EDT 2020

Differentiation Potential of Early- and Late-Passage Adipose-Derived Mesenchymal Stem Cells Cultured under Hypoxia and Normoxia.

Stem cells international

Zhao AG,Shah K,Freitag J,Cromer B,Sumer H

Published figure using CD73 monoclonal antibody (Product # 25-0739-42) in Flow Cytometry

Tue Oct 06 00:00:00 EDT 2020

Vasculogenesis from Human Dental Pulp Stem Cells Grown in Matrigel with Fully Defined Serum-Free Culture Media.

Biomedicines

Luzuriaga J,Irurzun J,Irastorza I,Unda F,Ibarretxe G,Pineda JR

Published figure using CD73 monoclonal antibody (Product # 25-0739-42) in Flow Cytometry

Mon Nov 09 00:00:00 EST 2020

PF-127 hydrogel plus sodium ascorbyl phosphate improves Wharton's jelly mesenchymal stem cell-mediated skin wound healing in mice.

Stem cell research & therapy

Deng Q,Huang S,Wen J,Jiao Y,Su X,Shi G,Huang J

Published figure using CD73 monoclonal antibody (Product # 25-0739-42) in Flow Cytometry

Fri Apr 03 00:00:00 EDT 2020

Chondrogenic Differentiation from Induced Pluripotent Stem Cells Using Non-Viral Minicircle Vectors.

Cells

Rim YA,Nam Y,Park N,Jung H,Lee K,Lee J,Ju JH

Published figure using CD73 monoclonal antibody (Product # 25-0739-42) in Flow Cytometry

Sun Mar 01 00:00:00 EST 2020

Prospective isolation of human fibroadipogenic progenitors with CD73.

Heliyon

Goloviznina NA,Xie N,Dandapat A,Iaizzo PA,Kyba M

Published figure using CD73 monoclonal antibody (Product # 25-0739-42) in Flow Cytometry

Wed Jul 01 00:00:00 EDT 2020

Circulating Exosomes Inhibit B Cell Proliferation and Activity.

Cancers

Schroeder JC,Puntigam L,Hofmann L,Jeske SS,Beccard IJ,Doescher J,Laban S,Hoffmann TK,Brunner C,Theodoraki MN,Schuler PJ

Published figure using CD73 monoclonal antibody (Product # 25-0739-42) in Flow Cytometry

Wed Jul 29 00:00:00 EDT 2020

Impact of Fibronectin Knockout on Proliferation and Differentiation of Human Infrapatellar Fat Pad-Derived Stem Cells.

Frontiers in bioengineering and biotechnology

Wang Y,Fu Y,Yan Z,Zhang XB,Pei M

Published figure using CD73 monoclonal antibody (Product # 25-0739-42) in Flow Cytometry

Wed Dec 14 00:00:00 EST 2022

Therapeutic Effects of Human Urine-Derived Stem Cells in a Rat Model of Cisplatin-Induced Acute Kidney Injury In Vivo and In Vitro.

Stem cells international

Sun B,Luo X,Yang C,Liu P,Yang Y,Dong X,Yang Z,Xu J,Zhang Y,Li L

Published figure using CD73 monoclonal antibody (Product # 25-0739-42) in Flow Cytometry

Thu May 13 00:00:00 EDT 2021

Better therapeutic potential of bone marrow-derived mesenchymal stem cells compared with chorionic villi-derived mesenchymal stem cells in airway injury model.

Regenerative medicine

Ji S,Wu C,Tong L,Wang L,Zhou J,Chen C,Song Y

Published figure using CD73 monoclonal antibody (Product # 25-0739-42) in Flow Cytometry

Fri Mar 01 00:00:00 EST 2019

BMP14 induces tenogenic differentiation of bone marrow mesenchymal stem cells in vitro.

Experimental and therapeutic medicine

Wang D,Jiang X,Lu A,Tu M,Huang W,Huang P

Published figure using CD73 monoclonal antibody (Product # 25-0739-42) in Flow Cytometry

Wed Aug 01 00:00:00 EDT 2018

Human umbilical cord-derived mesenchymal stem cells ameliorate the enteropathy of food allergies in mice.

Experimental and therapeutic medicine

Yan N,Xu J,Zhao C,Wu Y,Gao F,Li C,Zhou W,Xiao T,Zhou X,Shao Q,Xia S

Published figure using CD73 monoclonal antibody (Product # 25-0739-42) in Flow Cytometry

Sat Dec 01 00:00:00 EST 2018

The influence of chemotherapy on adenosine-producing B cells in patients with head and neck squamous cell carcinoma.

Oncotarget

Ziebart A,Huber U,Jeske S,Laban S,Doescher J,Hoffmann TK,Brunner C,Jackson EK,Schuler PJ

Published figure using CD73 monoclonal antibody (Product # 25-0739-42) in Flow Cytometry

Fri Jan 19 00:00:00 EST 2018

A high-yield isolation and enrichment strategy for human lung microvascular endothelial cells.

Pulmonary circulation

Gaskill C,Majka SM

Published figure using CD73 monoclonal antibody (Product # 25-0739-42) in Flow Cytometry

Wed Mar 01 00:00:00 EST 2017

Human umbilical cord mesenchymal stem cells improve the reserve function of perimenopausal ovary via a paracrine mechanism.

Stem cell research & therapy

Li J,Mao Q,He J,She H,Zhang Z,Yin C

Published figure using CD73 monoclonal antibody (Product # 25-0739-42) in Flow Cytometry

Thu Mar 09 00:00:00 EST 2017

A Member of the Nuclear Receptor Superfamily, Designated as NR2F2, Supports the Self-Renewal Capacity and Pluripotency of Human Bone Marrow-Derived Mesenchymal Stem Cells.

Stem cells international

Zhu N,Wang H,Wang B,Wei J,Shan W,Feng J,Huang H

Published figure using CD73 monoclonal antibody (Product # 25-0739-42) in Flow Cytometry

Tue Jan 19 00:00:00 EST 2016

MIF Plays a Key Role in Regulating Tissue-Specific Chondro-Osteogenic Differentiation Fate of Human Cartilage Endplate Stem Cells under Hypoxia.

Stem cell reports

Yao Y,Deng Q,Song W,Zhang H,Li Y,Yang Y,Fan X,Liu M,Shang J,Sun C,Tang Y,Jin X,Liu H,Huang B,Zhou Y

Published figure using CD73 monoclonal antibody (Product # 25-0739-42) in Flow Cytometry

Tue Aug 09 00:00:00 EDT 2016

CRISPR/Cas9-based genetic correction for recessive dystrophic epidermolysis bullosa.

NPJ Regenerative medicine

Webber BR,Osborn MJ,McElroy AN,Twaroski K,Lonetree CL,DeFeo AP,Xia L,Eide C,Lees CJ,McElmurry RT,Riddle MJ,Kim CJ,Patel DD,Blazar BR,Tolar J

Published figure using CD73 monoclonal antibody (Product # 25-0739-42) in Flow Cytometry

Wed Sep 30 00:00:00 EDT 2020

Human Gingival Integration-Free iPSCs; a Source for MSC-Like Cells.

International journal of molecular sciences

Umezaki Y,Hashimoto Y,Nishishita N,Kawamata S,Baba S

Published figure using CD73 monoclonal antibody (Product # 25-0739-42) in Flow Cytometry

Mon Jun 15 00:00:00 EDT 2015

Epithelial cell differentiation of human mesenchymal stromal cells in decellularized lung scaffolds.

Tissue engineering. Part A

Mendez JJ,Ghaedi M,Steinbacher D,Niklason LE

Published figure using CD73 monoclonal antibody (Product # 25-0739-42) in Flow Cytometry

Sun Jun 01 00:00:00 EDT 2014

Hypoxia-cultured human adipose-derived mesenchymal stem cells are non-oncogenic and have enhanced viability, motility, and tropism to brain cancer.

Cell death & disease

Feng Y,Zhu M,Dangelmajer S,Lee YM,Wijesekera O,Castellanos CX,Denduluri A,Chaichana KL,Li Q,Zhang H,Levchenko A,Guerrero-Cazares H,Quiñones-Hinojosa A

Published figure using CD73 monoclonal antibody (Product # 25-0739-42) in Flow Cytometry

Thu Dec 11 00:00:00 EST 2014

Myoblasts derived from normal hESCs and dystrophic hiPSCs efficiently fuse with existing muscle fibers following transplantation.

Molecular therapy : the journal of the American Society of Gene Therapy

Goudenege S,Lebel C,Huot NB,Dufour C,Fujii I,Gekas J,Rousseau J,Tremblay JP

Published figure using CD73 monoclonal antibody (Product # 25-0739-42) in Flow Cytometry

Thu Nov 01 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: 5'-deoxynucleotidase; 5'-NT; 5'-nucleotidase; 5'-nucleotidase, ecto (CD73); CD73; Ecto-5'-nucleotidase; IMP-specific 5'-nucleotidase; Purine 5-Prime-Nucleotidase; Thymidylate 5'-phosphatase

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Gene Aliases: CALJA; CD73; E5NT; eN; eNT; NT; NT5; NT5E; NTE

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UniProt ID: (Human) P21589

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Entrez Gene ID: (Human) 4907

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Function(s)

nucleotide binding ferrous iron binding 5'-nucleotidase activity phosphodiesterase

Process(es)

purine nucleotide biosynthetic process purine nucleotide catabolic process AMP catabolic process DNA metabolic process leukocyte cell-cell adhesion brain development response to aluminum ion dephosphorylation adenosine biosynthetic process pyrimidine nucleoside catabolic process positive regulation of lipid biosynthetic process negative regulation of inflammatory response

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CD73 Monoclonal Antibody (AD2), PE-Cyanine7, eBioscience™

CD73 Antibody (25-0739-42) in Flow

Product Details

25-0739-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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