Invitrogen

Axl Monoclonal Antibody (DS7HAXL), PE-Cyanine7, eBioscience™

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

2 Published Figures

3 References

View all (66) AXL antibodies

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FIGURE: 1 / 6

Axl Antibody (25-1087-42) in Flow

Normal human peripheral blood cells were stained with HLA-DR Monoclonal Antibody, FITC (Product # 11-9956-42), CD1c Monoclonal Antibody, APC (Product # 17-0015-42), CD11c Monoclonal Antibody, eFluor 450 (Product # 49-0116-42), and Mouse IgG1 kappa Isotype Control, PE-Cyanine7 (Product # 25-4714-82) (left) or Axl Monoclonal Antibody, PE-Cyanine7 (right). CD11c+/CD14- cells were used for analysis.

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Axl Antibody — Staining of human peripheral blood mononuclear cells. As expected based on known expression patterns, Axl clone DS7HAXL stains a subset of CD11c+/CD14- dendritic cells. Details: Normal human PBMC were surface stained with Axl clone DS7HAXL and co-stained with CD14, CD11c, CD1c and HLA-DR. CD11c+/CD14- cells were used for analysis. {RE}

Product Details

25-1087-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.06 µg)/test

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human

Published species

Not Applicable

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-Cyanine7, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

DS7HAXL

Conjugate

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

Excitation/Emission Max

569/780 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2723959

Product Specific Information

This DS7HAXL monoclonal antibody recognizes human Axl, a member of the TAM family of tyrosine kinase receptors that also includes MerTK and Tyro3. This DS7HAXL antibody will work in flow cytometry on both native and paraformaldehyde-fixed cells. It has also been shown to stain formalin-fixed, paraffin-embedded tissues for IHC analysis.

Applications Reported: This DS7HAXL antibody has been reported for use in flow cytometric analysis.

Applications Tested: This DS7HAXL antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-34) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Target Information

AXL is a chronic myelogenous leukemia-associated oncogene that is also associated with colon cancer and melanoma. The AXL protein is a receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding growth factors like vitamin K-dependent protein growth-arrest-specific gene 6, and is involved in the stimulation of cell proliferation. TAM receptors are also involved in phagocytosis of apoptotic cells. Mice lacking all three TAM receptors have several degenerative phenotypes linked to inefficient removal of apoptotic cells and membranes (e.g., in the retina and the male reproductive tract) and develop a severe autoimmune phenotype akin to systemic lupus erythematosus, including the production of broad spectrum auto-antibodies. In addition, Axl may function as a putative entry receptor for filoviruses. Axl is also used to identify a specific subpopulation of human blood dendritic cells, also referred to as Siglec 6+/Axl+ dendritic cells. Cellular expression of Axl can be upregulated by TLR ligands, such as LPS or poly I:C. Soluble Axl is generated by proteolytic cleavage of the membrane form. Increased plasma levels may be indicative of inflammation and cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Oral Epithelial Cells Expressing Low or Undetectable Levels of Human Angiotensin-Converting Enzyme 2 Are Susceptible to SARS-CoV-2 Virus Infection In Vitro.

Pathogens (Basel, Switzerland)

Ebraham L,Xu C,Wang A,Hernandez C,Siclari N,Rajah D,Walter L,Marras SAE,Tyagi S,Fine DH,Daep CA,Chang TL

Published figure using Axl monoclonal antibody (Product # 25-1087-42) in Flow Cytometry

Mon Jun 19 00:00:00 EDT 2023

Single-cell profiling of alveolar rhabdomyosarcoma reveals RAS pathway inhibitors as cell-fate hijackers with therapeutic relevance.

Science advances

Danielli SG,Porpiglia E,De Micheli AJ,Navarro N,Zellinger MJ,Bechtold I,Kisele S,Volken L,Marques JG,Kasper S,Bode PK,Henssen AG,Gürgen D,Delattre O,Surdez D,Roma J,Bühlmann P,Blau HM,Wachtel M,Schäfer BW

Published figure using Axl monoclonal antibody (Product # 25-1087-42) in Flow Cytometry

Fri Feb 10 00:00:00 EST 2023

Remodelling of tumour microenvironment by microwave ablation potentiates immunotherapy of AXL-specific CAR T cells against non-small cell lung cancer.

Nature communications

Cao B,Liu M,Wang L,Zhu K,Cai M,Chen X,Feng Y,Yang S,Fu S,Zhi C,Ye X,Zhang J,Zhang Z,Yang X,Zhao M,Wu Q,Xu L,Yang L,Lian H,Zhao Q,Zhang Z

Published figure using Axl monoclonal antibody (Product # 25-1087-42) in Flow Cytometry

Wed Oct 19 00:00:00 EDT 2022

Bioinformatics

Protein Aliases: AXL oncogene; AXL transforming sequence/gene; AZF1; oncogene AXL; sAXL; soluble AXL; Tyrosine-protein kinase receptor UFO

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Gene Aliases: ARK; AXL; JTK11; Tyro7; UFO

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UniProt ID: (Human) P30530

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Entrez Gene ID: (Human) 558

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Function(s)

virus receptor activity phosphatidylserine binding protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity protein binding ATP binding protein heterodimerization activity transmembrane signal receptor

Process(es)

neuron migration natural killer cell differentiation positive regulation of cytokine-mediated signaling pathway blood vessel remodeling phagocytosis inflammatory response signal transduction spermatogenesis peptidyl-tyrosine phosphorylation forebrain cell migration platelet activation cellular response to extracellular stimulus negative regulation of interferon-gamma production negative regulation of tumor necrosis factor production positive regulation of natural killer cell differentiation secretion by cell erythrocyte homeostasis substrate adhesion-dependent cell spreading cellular response to interferon-alpha ovulation cycle apoptotic cell clearance protein kinase B signaling negative regulation of neuron apoptotic process innate immune response viral entry into host cell vascular endothelial growth factor receptor signaling pathway cell maturation positive regulation of pinocytosis negative regulation of lymphocyte activation positive regulation of protein kinase B signaling vagina development cellular response to lipopolysaccharide dendritic cell differentiation negative regulation of dendritic cell apoptotic process

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Axl Monoclonal Antibody (DS7HAXL), PE-Cyanine7, eBioscience™

Axl Antibody (25-1087-42) in Flow

Product Details

25-1087-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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