Invitrogen

MERTK Monoclonal Antibody (DS5MMER), PE-Cyanine7, eBioscience™

6 Published Figures

13 References

View all (70) MERTK antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (6)

FIGURE: 1 / 7

MERTK Antibody (25-5751-82) in Flow

Staining of mouse resident peritoneal exudate cells with Anti-Mouse CD11b APC (Product # 17-0112-82) and 0.25 µg of Rat IgG2a K Isotype Control PE-Cyanine7 (Product # 25-4321-82) (left) or 0.25 µg of Anti-Mouse MerTK PE-Cyanine7 (right). Total viable cells were used for analysis.

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Product Details

25-5751-82

Applications

Tested Dilution

Publications

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Flow Cytometry (Flow)

0.5 µg/test

View 12 publications 12 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PE-Cyanine7, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

DS5MMER

Immunogen

FC-tagged full protein expressed in insect cells

View immunogen

Conjugate

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

Excitation/Emission Max

569/780 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2573466

Product Specific Information

Description: This DS5MMER monoclonal antibody recognizes mouse MerTK, a 170-210 kDa member of the TAM family of tyrosine kinase receptors that also includes Axl and Tyro3. MerTK is expressed on tissue macrophages and is involved in the removal of apoptotic cells. This process relies on two soluble ligands of MerTK, Protein S and Gas6 that bind to phosphatidylserine found on the outer leaflet of the plasma membrane of cells undergoing apoptosis. Upon binding these ligands, MerTK undergoes autophosphorylation at multiple tyrosine residues that activate the PI3K and Akt pathways. This results in the phagocytosis of apoptotic cells and also results in the direct inhibition of TLR-induced production of pro-inflammatory cytokines. In addition, MerTK may function as a putative entry receptor for filoviruses. Deficiency of MerTK causes general autoimmunity, inflammation and accumulation of apoptotic bodies. MerTK is constitutively released from the cell surface by metalloproteinases and thus is present in the serum and culture medium. This process can be enhanced by stimulation with LPS. MerTK can be used to help discriminate macrophages from dendritic cells. MerTK is often expressed on malignant cells and may be implicated in immune evasion.

The DS5MMER antibody will work on paraformaldehyde-fixed cells.

Applications Reported: This DS5MMER antibody has been reported for use in flow cytometric analysis.

Applications Tested: This DS5MMER antibody has been tested by flow cytometric analysis of mouse resident peritoneal cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

MERTK (cMER) is a tyrosine kinase proto-oncogene and is involved in the retinal pigment epithelium (RPE) phagocytosis pathway, which is implicated in human retinal disease. Regulates many physiological processes including cell survival, migration, differentiation, and phagocytosis of apoptotic cells (efferocytosis). Ligand binding at the cell surface induces autophosphorylation of MERTK on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with GRB2 or PLCG2 and induces phosphorylation of MAPK1, MAPK2, FAK/PTK2 or RAC1. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization and engulfment. Functions in the retinal pigment epithelium (RPE) as a regulator of rod outer segments fragments phagocytosis. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

AXL Expression on Homeostatic Resident Liver Macrophages Is Reduced in Cirrhosis Following GAS6 Production by Hepatic Stellate Cells.

Cellular and molecular gastroenterology and hepatology

Pop OT,Geng A,Flint E,Singanayagam A,Ercan C,Possamai L,Patel VC,Kuenzler P,Meier MA,Soysal S,Hruz P,Kollmar O,Tatham KC,Ward JK,Müllhaupt B,Weber A,Wendon J,Niess JH,Heim M,Semela D,Weston C,Antoniades CG,Terracciano LM,Triantafyllou E,Brenig RG,Bernsmeier C

Published figure using MERTK monoclonal antibody (Product # 25-5751-82) in Flow Cytometry

Mon Jun 26 00:00:00 EDT 2023

Lactation-associated macrophages exist in murine mammary tissue and human milk.

Nature immunology

Cansever D,Petrova E,Krishnarajah S,Mussak C,Welsh CA,Mildenberger W,Mulder K,Kreiner V,Roussel E,Stifter SA,Andreadou M,Zwicky P,Jurado NP,Rehrauer H,Tan G,Liu Z,Blériot C,Ronchi F,Macpherson AJ,Ginhoux F,Natalucci G,Becher B,Greter M

25-5751-82 was used in Flow cytometry/Cell sorting to reveal the emergence of unique macrophages in the mammary gland and milk during lactation.

Sat Jul 01 00:00:00 EDT 2023

Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition).

European journal of immunology

Cossarizza A,Chang HD,Radbruch A,Abrignani S,Addo R,Akdis M,Andrä I,Andreata F,Annunziato F,Arranz E,Bacher P,Bari S,Barnaba V,Barros-Martins J,Baumjohann D,Beccaria CG,Bernardo D,Boardman DA,Borger J,Böttcher C,Brockmann L,Burns M,Busch DH,Cameron G,Cammarata I,Cassotta A,Chang Y,Chirdo FG,Christakou E,Čičin-Šain L,Cook L,Corbett AJ,Cornelis R,Cosmi L,Davey MS,De Biasi S,De Simone G,Del Zotto G,Delacher M,Di Rosa F,Di Santo J,Diefenbach A,Dong J,Dörner T,Dress RJ,Dutertre CA,Eckle SBG,Eede P,Evrard M,Falk CS,Feuerer M,Fillatreau S,Fiz-Lopez A,Follo M,Foulds GA,Fröbel J,Gagliani N,Galletti G,Gangaev A,Garbi N,Garrote JA,Geginat J,Gherardin NA,Gibellini L,Ginhoux F,Godfrey DI,Gruarin P,Haftmann C,Hansmann L,Harpur CM,Hayday AC,Heine G,Hernández DC,Herrmann M,Hoelsken O,Huang Q,Huber S,Huber JE,Huehn J,Hundemer M,Hwang WYK,Iannacone M,Ivison SM,Jäck HM,Jani PK,Keller B,Kessler N,Ketelaars S,Knop L,Knopf J,Koay HF,Kobow K,Kriegsmann K,Kristyanto H,Krueger A,Kuehne JF,Kunze-Schumacher H,Kvistborg P,Kwok I,Latorre D,Lenz D,Levings MK,Lino AC,Liotta F,Long HM,Lugli E,MacDonald KN,Maggi L,Maini MK,Mair F,Manta C,Manz RA,Mashreghi MF,Mazzoni A,McCluskey J,Mei HE,Melchers F,Melzer S,Mielenz D,Monin L,Moretta L,Multhoff G,Muñoz LE,Muñoz-Ruiz M,Muscate F,Natalini A,Neumann K,Ng LG,Niedobitek A,Niemz J,Almeida LN,Notarbartolo S,Ostendorf L,Pallett LJ,Patel AA,Percin GI,Peruzzi G,Pinti M,Pockley AG,Pracht K,Prinz I,Pujol-Autonell I,Pulvirenti N,Quatrini L,Quinn KM,Radbruch H,Rhys H,Rodrigo MB,Romagnani C,Saggau C,Sakaguchi S,Sallusto F,Sanderink L,Sandrock I,Schauer C,Scheffold A,Scherer HU,Schiemann M,Schildberg FA,Schober K,Schoen J,Schuh W,Schüler T,Schulz AR,Schulz S,Schulze J,Simonetti S,Singh J,Sitnik KM,Stark R,Starossom S,Stehle C,Szelinski F,Tan L,Tarnok A,Tornack J,Tree TIM,van Beek JJP,van de Veen W,van Gisbergen K,Vasco C,Verheyden NA,von Borstel A,Ward-Hartstonge KA,Warnatz K,Waskow C,Wiedemann A,Wilharm A,Wing J,Wirz O,Wittner J,Yang JHM,Yang J

25-5751-82 was used in Flow cytometry/Cell sorting to provide the key aspects to consider when performing flow cytometry experiments.

Wed Dec 01 00:00:00 EST 2021

Ligand-dependent kinase activity of MERTK drives efferocytosis in human iPSC-derived macrophages.

Cell death & disease

Wanke F,Gutbier S,Rümmelin A,Steinberg M,Hughes LD,Koenen M,Komuczki J,Regan-Komito D,Wagage S,Hesselmann J,Thoma R,Brugger D,Christopeit T,Wang H,Point F,Hallet R,Ghosh S,Rothlin CV,Patsch C,Geering B

Published figure using MERTK monoclonal antibody (Product # 25-5751-82) in Flow Cytometry

Tue May 25 00:00:00 EDT 2021

Transmission of trained immunity and heterologous resistance to infections across generations.

Nature immunology

Katzmarski N,Domínguez-Andrés J,Cirovic B,Renieris G,Ciarlo E,Le Roy D,Lepikhov K,Kattler K,Gasparoni G,Händler K,Theis H,Beyer M,van der Meer JWM,Joosten LAB,Walter J,Schultze JL,Roger T,Giamarellos-Bourboulis EJ,Schlitzer A,Netea MG

25-5751-82 was used in Flow Cytometry to provide evidence for inheritance of trained immunity in mammals, enhancing protection against infections.

Mon Nov 01 00:00:00 EDT 2021

A Two-Cell Model for IL-1β Release Mediated by Death-Receptor Signaling.

Cell reports

Donado CA,Cao AB,Simmons DP,Croker BA,Brennan PJ,Brenner MB

25-5751 was used in Flow cytometry/Cell sorting to describe an alternative, two-cell model for IL-1β release where invariant natural killer T (iNKT) cells use the death receptor pathway to instruct antigen-presenting cells to secrete IL-1β.

Tue Apr 07 00:00:00 EDT 2020

The cis-Regulatory Atlas of the Mouse Immune System.

Cell

Yoshida H,Lareau CA,Ramirez RN,Rose SA,Maier B,Wroblewska A,Desland F,Chudnovskiy A,Mortha A,Dominguez C,Tellier J,Kim E,Dwyer D,Shinton S,Nabekura T,Qi Y,Yu B,Robinette M,Kim KW,Wagers A,Rhoads A,Nutt SL,Brown BD,Mostafavi S,Buenrostro JD,Benoist C

25-5751-82 was used in Flow Cytometry to generate matched epigenome and transcriptome measurements in 86 primary cell types that span the mouse immune system and its differentiation cascade.

Thu Feb 07 00:00:00 EST 2019

Pro-inflammatory Aorta-Associated Macrophages Are Involved in Embryonic Development of Hematopoietic Stem Cells.

Immunity

Mariani SA,Li Z,Rice S,Krieg C,Fragkogianni S,Robinson M,Vink CS,Pollard JW,Dzierzak E

25-5751-82 was used in Immunohistochemistry (Frozen) to show here that macrophage appearance in the AGM was dependent on the chemokine receptor Cx3cr1.

Tue Jun 18 00:00:00 EDT 2019

TIV Vaccination Modulates Host Responses to Influenza Virus Infection that Correlate with Protection against Bacterial Superinfection.

Vaccines

Choi A,Christopoulou I,Saelens X,García-Sastre A,Schotsaert M

25-5751 was used in Flow cytometry/Cell sorting to establish a mouse vaccination model that allows control of disease severity after influenza virus infection despite inefficient induction of virus-neutralizing antibody titers by vaccination.

Thu Sep 12 00:00:00 EDT 2019

High-Dimensional Single-Cell Mapping of Central Nervous System Immune Cells Reveals Distinct Myeloid Subsets in Health, Aging, and Disease.

Immunity

Mrdjen D,Pavlovic A,Hartmann FJ,Schreiner B,Utz SG,Leung BP,Lelios I,Heppner FL,Kipnis J,Merkler D,Greter M,Becher B

Tue Mar 20 00:00:00 EDT 2018

Protective Role of the MER Tyrosine Kinase via Efferocytosis in Rheumatoid Arthritis Models.

Frontiers in immunology

Waterborg CEJ,Beermann S,Broeren MGA,Bennink MB,Koenders MI,van Lent PLEM,van den Berg WB,van der Kraan PM,van de Loo FAJ

Published figure using MERTK monoclonal antibody (Product # 25-5751-82) in Flow Cytometry

Thu Jun 13 00:00:00 EDT 2019

iRhom2-mediated proinflammatory signalling regulates heart repair following myocardial infarction.

JCI insight

Barnette DN,Cahill TJ,Gunadasa-Rohling M,Carr CA,Freeman M,Riley PR

25-5751 was used in Flow cytometry/Cell sorting to reveal a requirement for an iRhom2-mediated proinflammatory response during downstream scarring and fibrosis, which is driven in part by TNF-α signaling.

Thu Feb 08 00:00:00 EST 2018

Aryl Hydrocarbon Receptor Controls Monocyte Differentiation into Dendritic Cells versus Macrophages.

Immunity

Goudot C,Coillard A,Villani AC,Gueguen P,Cros A,Sarkizova S,Tang-Huau TL,Bohec M,Baulande S,Hacohen N,Amigorena S,Segura E

25-5751 was used in Flow cytometry/Cell sorting to elucidate the role of the aryl hydrocarbon receptor as a molecular switch for monocyte fate specification in response to micro-environmental factors.

Tue Sep 19 00:00:00 EDT 2017

Bioinformatics

Protein Aliases: MGC133349; Proto-oncogene c-Mer; proto-oncogene tyrosine-protein kinase Mer; Receptor tyrosine kinase MerTK; sMER; sMERTK; soluble MER; soluble MERTK; Tyro 12; Tyrosine-protein kinase Mer

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Gene Aliases: Eyk; Mer; Mertk; nmf12; Nyk

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UniProt ID: (Mouse) Q60805

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Entrez Gene ID: (Mouse) 17289

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Function(s)

nucleotide binding protein kinase activity protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity ATP binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups transmembrane signal receptor

Process(es)

natural killer cell differentiation protein phosphorylation phagocytosis spermatogenesis phosphorylation platelet activation secretion by cell substrate adhesion-dependent cell spreading apoptotic cell clearance protein kinase B signaling positive regulation of phagocytosis negative regulation of lymphocyte activation retina development in camera-type eye vagina development negative regulation of leukocyte apoptotic process

Performance Guarantee

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MERTK Monoclonal Antibody (DS5MMER), PE-Cyanine7, eBioscience™

MERTK Antibody (25-5751-82) in Flow

Product Details

25-5751-82

Immunohistochemistry (Frozen) (IHC (F))

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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