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Invitrogen
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Description: The monoclonal antibody bg1fpj recognizes mouse CD369, also known as Dectin-1 or CLEC7A, a type II transmembrane protein in the C-type lectin family. The ligand is beta1, 3-glycan. Beta glycans are a main component in polysaccharides and specifically beta1,3-linked carbohydrates are found in the cell wall of fungi, plants and some bacteria. CD369 has been found to bind Candida, Aspergillis, Pneumocystis, Coccidioides and Mycobacterium. Calcium dependent binding to ligand signals through syk via the ITAM domain in the cytoplasmic portion and results in endocyotosis/phagocytosis and production of cytokines (IL-2, IL-6, IL-10, TNF alpha, IL-1 beta, IL-23). A direct role in Th17 and Th1 differentiation has been attributed to CD369 signaling. CD369 is expressed predominantly on myeloid cells (neutrophils, monocytes, macrophages, and dendritic cells). Low levels have also been reported on lymphocytes and mucosal epithelium.
Applications Reported: This bg1fpj antibody has been reported for use in flow cytometric analysis.
Applications Tested: This bg1fpj antibody has been tested by flow cytometric analysis of mouse lysed whole blood cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
CLEC7A, also known as dectin-1, is a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily and is predominantly expressed on myeloid cells. It is a small glycoprotein type II membrane receptor with an extracellular C-type lectin-like domain fold and a cytoplasmic domain with an immunoreceptor tyrosine-based activation motif (ITAM). CLEC7A functions as a pattern-recognition receptor that recognizes a variety of beta-1,3-linked and beta-1,6-linked glucans from fungi and plants, and in this way plays a role in innate immune response. Upon fungal exposure, CLEC7A activates Syk tyrosine kinase, triggering a massive oxidative burst through the formation of reactive oxygen species.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Protein Aliases: beta-glucan receptor; C-type (calcium dependent, carbohydrate recognition domain) lectin, superfamily member 12; C-type lectin domain family 7 member A; C-type lectin superfamily member 12; DC-associated C-type lectin 1; dectin-1; dendritic cell-associated C-type lectin 1
Gene Aliases: beta-GR; BGR; Clecsf12
Entrez Gene ID: (Mouse) 56644
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