Invitrogen

IL-4 Monoclonal Antibody (BVD6-24G2), PE-Cyanine7, eBioscience™

5 Published Figures

20 References

View all (109) IL-4 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Published Figures (5)

FIGURE: 1 / 5

IL-4 Antibody (25-7042-42) in Flow

Journal of cellular and molecular medicine 2020 - 3 FIGURE Effects of DTA-64 on the Th1/Th2/Th17 balance in vivo. Flow cytometric analysis of CD4 + T cell and Th1/Th2/Th17 subtypes was performed in BAL cells, mLNs and splenocytes. (A) CD3e+/CD4+ cells in the BAL cells, mLNs and splenocytes were selected. (B) Proportion of CD3e+/CD4+ cells was displayed by histogram. (C) Th1/Th2/Th17 cytokines within the CD3e+ CD4+ cells in the BAL cells were selected. (D) ... View More

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Product Details

25-7042-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.5 µg)/test

View 19 publications 19 publications

In vitro Assay (IV)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG1, kappa

Recommended Isotype Control

Rat IgG1 kappa Isotype Control (eBRG1), PE-Cyanine7, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

BVD6-24G2

Conjugate

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

Excitation/Emission Max

569/780 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1659721

Product Specific Information

Description: The BVD6-24G2 antibody reacts with mouse interleukin-4 (IL-4), a 14 kDa cytokine secreted by Th2 cells.

Applications Reported:This BVD6-24G2 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: Has been pre-titrated and tested intracellular staining and flow cytometric analysis of restimulated mouse splenocytes cultures. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

An enhanced level of LAMP-2A participates in CD4⁺T cell hyperactivity in patients with primary biliary cholangitis.

Annals of translational medicine

Sun K,Ma S,Tian S,Zhang M,Liu Y,Li B,Zhou X,Zheng X,Zhou X,Wang L,Han Y

25-7042-42 was used in Flow Cytometry to evaluate the immunobiology of CD4+T cells in patients with primary biliary cholangitis (PBC), chronic hepatitis B, and healthy control subjects, and investigate the activation responses of PBC-naïve CD4+T cells by stimulation in vitro and tested the changes caused by deleting the gene encoding LAMP-2A.

Fri Jan 01 00:00:00 EST 2021

NLRX1 is a key regulator of immune signaling during invasive pulmonary aspergillosis.

PLoS pathogens

Kastelberg B,Tubau-Juni N,Ayubi T,Leung A,Leber A,Hontecillas R,Bassaganya-Riera J,Kale SD

Published figure using IL-4 monoclonal antibody (Product # 25-7042-42) in Flow Cytometry

Tue Sep 01 00:00:00 EDT 2020

DEK-targeting aptamer DTA-64 attenuates bronchial EMT-mediated airway remodelling by suppressing TGF-β1/Smad, MAPK and PI3K signalling pathway in asthma.

Journal of cellular and molecular medicine

Song Y,Wang Z,Jiang J,Piao Y,Li L,Xu C,Piao H,Li L,Yan G

Published figure using IL-4 monoclonal antibody (Product # 25-7042-42) in Flow Cytometry

Tue Dec 01 00:00:00 EST 2020

Betulin from Hedyotis hedyotidea ameliorates concanavalin A-induced and T cell-mediated autoimmune hepatitis in mice.

Acta pharmacologica Sinica

Zhou YQ,Weng XF,Dou R,Tan XS,Zhang TT,Fang JB,Wu XW

Wed Feb 01 00:00:00 EST 2017

mTORC2 regulates multiple aspects of NKT-cell development and function.

European journal of immunology

Sklarz T,Guan P,Gohil M,Cotton RM,Ge MQ,Haczku A,Das R,Jordan MS

25-7042 was used in Flow cytometry/Cell sorting to define the molecular pathway and role of Rictor in regulating invariant NKT-cell fate.

Wed Mar 01 00:00:00 EST 2017

TIM4-TIM1 interaction modulates Th2 pattern inflammation through enhancing SIRT1 expression.

International journal of molecular medicine

Hu T,Fan X,Ma L,Liu J,Chang Y,Yang P,Qiu S,Chen T,Yang L,Liu Z

25-7042 was used in Flow cytometry/Cell sorting to demonstrate that the interaction of T-cell immunoglobulin and mucin domain (TIM)4/TIM1 promotes T helper 2-cell proliferation through enhancing silent information regulator 1 expression in mice with nasal allergic rhinitis.

Wed Nov 01 00:00:00 EDT 2017

Induced Treg Cells Augment the Th17-Mediated Intestinal Inflammatory Response in a CTLA4-Dependent Manner.

PloS one

Watanabe N,Kaminuma O,Kitamura N,Hiroi T

25-7042 was used in Flow cytometry/Cell sorting to study whether Th17 and Tregs that promote and suppress inflammatory responses, respectively, work with or against each other in immune reactions, showing that cotransfer of Th17 and induced Tregs led to increased inflammation, and blockage of CTLA4 led to abrogation of this effect.

Mon Aug 01 00:00:00 EDT 2016

Antiapoptotic Mcl-1 is critical for the survival and niche-filling capacity of Foxp3⁺ regulatory T cells.

Nature immunology

Pierson W,Cauwe B,Policheni A,Schlenner SM,Franckaert D,Berges J,Humblet-Baron S,Schönefeldt S,Herold MJ,Hildeman D,Strasser A,Bouillet P,Lu LF,Matthys P,Freitas AA,Luther RJ,Weaver CT,Dooley J,Gray DH,Liston A

25-7042 was used in Flow cytometry/Cell sorting to study the regulation of the Foxp3+ T cell pool by proliferation and apoptosis, and specifically the role of antiapoptoic Mcl-1 in T cell survival.

Sun Sep 01 00:00:00 EDT 2013

Loss of T cell microRNA provides systemic protection against autoimmune pathology in mice.

Journal of autoimmunity

Tian L,De Hertogh G,Fedeli M,Staats KA,Schonefeldt S,Humblet-Baron S,Van Den Bosch L,Dellabona P,Dooley J,Liston A

25-7042 was used in Flow cytometry/Cell sorting to elucidate the dominant effects of the T cell microRNA expression network and explore it as a potential therapeutic target for autoimmune diseases.

Wed Feb 01 00:00:00 EST 2012

IL-10+ CTLA-4+ Th2 inhibitory cells form in a Foxp3-independent, IL-2-dependent manner from Th2 effectors during chronic inflammation.

Journal of immunology (Baltimore, Md. : 1950)

Altin JA,Goodnow CC,Cook MC

25-7042 was used in Flow cytometry/Cell sorting to investigate the mechanism and molecules involved in the formation of IL10+CTLA-4+Th2 inhibitory cells during chronic inflammation.

Fri Jun 01 00:00:00 EDT 2012

Epithelial cell-intrinsic Notch signaling plays an essential role in the maintenance of gut immune homeostasis.

Journal of immunology (Baltimore, Md. : 1950)

Obata Y,Takahashi D,Ebisawa M,Kakiguchi K,Yonemura S,Jinnohara T,Kanaya T,Fujimura Y,Ohmae M,Hase K,Ohno H

25-7042 was used in Flow cytometry/Cell sorting to demonstrate that mice with an IEC-specific deletion in a TF that mediates Notch signalling spontaneously develop colitis.

Thu Mar 01 00:00:00 EST 2012

The acute environment, rather than T cell subset pre-commitment, regulates expression of the human T cell cytokine amphiregulin.

PloS one

Qi Y,Operario DJ,Georas SN,Mosmann TR

25-7042 was used in in vitro experiments to collate a detailed analysis of the regulation of Amphiregulin expression by human T cell subsets.

Thu Dec 13 00:00:00 EST 2012

The acute environment, rather than T cell subset pre-commitment, regulates expression of the human T cell cytokine amphiregulin.

PloS one

Qi Y,Operario DJ,Georas SN,Mosmann TR

25-7042 was used in in vitro experiments to collate a detailed analysis of the regulation of Amphiregulin expression by human T cell subsets.

Thu Dec 13 00:00:00 EST 2012

Soluble MOG35-55/I-A(b) dimers ameliorate experimental autoimmune encephalomyelitis by reducing encephalitogenic T cells.

PloS one

Gong Y,Wang Z,Liang Z,Duan H,Ouyang L,Yu Q,Xu Z,Shen G,Weng X,Wu X

25-7042 was used in Flow cytometry/Cell sorting to demonstrate that soluble divalent MHC class II molecules can abrogate pathogenic T cells in experimental autoimmune encephalomyelitis.

Fri Jul 19 00:00:00 EDT 2013

IL-17-producing invariant NKT cells in lymphoid organs are recent thymic emigrants identified by neuropilin-1 expression.

Blood

Milpied P,Massot B,Renand A,Diem S,Herbelin A,Leite-de-Moraes M,Rubio MT,Hermine O

25-7042 was used in Flow cytometry/Cell sorting to determine the function of recent thymic emigrant invariant natural killer (RTE TiNKT) cells in naive mice.

Thu Sep 15 00:00:00 EDT 2011

Foxp3⁺ regulatory T cells exert asymmetric control over murine helper responses by inducing Th2 cell apoptosis.

Blood

Tian L,Altin JA,Makaroff LE,Franckaert D,Cook MC,Goodnow CC,Dooley J,Liston A

25-7042 was used in Immunocytochemistry to demonstrate that Th2-driven diseases may be more responsive to regulatory T-cell manipulation.

Thu Aug 18 00:00:00 EDT 2011

Decreased T-cell receptor signaling through CARD11 differentially compromises forkhead box protein 3-positive regulatory versus T(H)2 effector cells to cause allergy.

The Journal of allergy and clinical immunology

Altin JA,Tian L,Liston A,Bertram EM,Goodnow CC,Cook MC

25-7042 was used in Flow cytometry/Cell sorting to find that reduction in the efficiency of T-cell receptor (TCR)- nuclear factor κB (NF-κB) signalling has graded effects on T-cell activation and Foxp3(+) regulatory T-cell suppression causing allergy.

Sun May 01 00:00:00 EDT 2011

Human basophils express amphiregulin in response to T cell-derived IL-3.

The Journal of allergy and clinical immunology

Qi Y,Operario DJ,Oberholzer CM,Kobie JJ,Looney RJ,Georas SN,Mosmann TR

25-7042 was used in Flow cytometry/Cell sorting to identify the human peripheral blood cell population expressing amphiregulin, showing that human basophils express amphiregulin in response to T cell-derived IL-3.

Wed Dec 01 00:00:00 EST 2010

Wiskott-Aldrich syndrome protein is required for homeostasis and function of invariant NKT cells.

Journal of immunology (Baltimore, Md. : 1950)

Astrakhan A,Ochs HD,Rawlings DJ

25-7042 was used in Flow cytometry/Cell sorting to investigate the role for WASp within the iNKT lineage.

Mon Jun 15 00:00:00 EDT 2009

IL-9- and mast cell-mediated intestinal permeability predisposes to oral antigen hypersensitivity.

The Journal of experimental medicine

Forbes EE,Groschwitz K,Abonia JP,Brandt EB,Cohen E,Blanchard C,Ahrens R,Seidu L,McKenzie A,Strait R,Finkelman FD,Foster PS,Matthaei KI,Rothenberg ME,Hogan SP

25-7042 was used in Flow cytometry/Cell sorting to demonstrate a central role for IL-9 and mast cells in experimental intestinal permeability in oral antigen sensitisation.

Mon Apr 14 00:00:00 EDT 2008

Bioinformatics

Protein Aliases: B-cell growth factor 1; B-cell IgG differentiation factor; B-cell stimulatory factor 1; BSF-1; H-IL-4; IGG1 induction factor; IL-4; ILN; Interleukin; Interleukin-4; Interleukin4; Lymphocyte stimulatory factor 1; M-IL-4; MGC79402

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Gene Aliases: BSF-1; Il-4; Il4

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UniProt ID: (Mouse) P07750

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Entrez Gene ID: (Mouse) 16189

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Function(s)

cytokine activity cytokine receptor binding interleukin-4 receptor binding growth factor activity interleukin superfamily

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IL-4 Monoclonal Antibody (BVD6-24G2), PE-Cyanine7, eBioscience™

IL-4 Antibody (25-7042-42) in Flow

Product Details

25-7042-42

Flow Cytometry (Flow)

In vitro Assay (IV)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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