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Invitrogen
HA Tag Monoclonal Antibody (2-2.2.14), DyLight™ 800 4X PEG
FIGURE: 1 / 2
HA Tag Antibody (26183-D800) in WB
Western blot analysis of HA Epitope Tag was performed by loading various amounts of E. coli lysate containing a multi-epitope tagged protein per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane and blocked with SEA BLOCK (Product # 37527) for at least 1 hour. The membrane was probed with a DyLight 800-conjugated HA Epitope Tag monoclonal antibody (Product # 26183-D800) at a dilution of 1:1000 for 1 hour at room temperature on a rocking platform and washed in TBS-0.1% Tween-20. Detection ... View More
Product Details
26183-D800
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Tag
Published species
Not Applicable
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
2-2.2.14
Immunogen
HA peptide YPYDVPDYA derivitized to ovalbumin.
Conjugate
Excitation/Emission Max
789/794 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
MES with proprietary stabilizer
Contains
no preservative
Storage conditions
4° C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2533055
Product Specific Information
26183-D800 has been successfully used for Western blotting. DyLight 800 4X PEG has an excitation/emission of 783/797 nm.
HA Synthetic Peptide (Product # 26184) is available for use in neutralization and control experiments and to competitively elute HA-tagged fusion proteins from immobilized anti-HA affinity resin.
Target Information
HA Tag (hemagglutinin tag) is an epitope tag that provides a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. HA (hemagglutinin) is a surface glycoprotein derived from influenza hemagglutinin, and is commonly expressed as a tag in conjunction with proteins to aid in identification and purification of the tagged protein for functional analysis. HA tag antibodies can be used to facilitate protein detection or co-immunopreciptation of tagged proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: HA; HA Epitope Tag; HA Tag; HA-tag; hemagglutinin
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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