Invitrogen

CD117 (c-Kit) Monoclonal Antibody (2B8), Brilliant Ultra Violet™ 615, eBioscience™

View all (212) c-Kit (CD117) antibodies
Datasheet
Protocols
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Datasheet
Protocols
Questions & Answers

Product Details

366-1171-82

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

0.5 µg/test
-
Product Specifications

Species Reactivity

Mouse, Pig

Host/Isotype

Rat / IgG2b, kappa

Recommended Isotype Control

Rat IgG2b kappa Isotype Control (eB149/10H5), Brilliant Ultra Violet™ 615, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

2B8

Conjugate

Brilliant Ultra Violet™ 615 Brilliant Ultra Violet™ 615 Brilliant Ultra Violet™ 615

Excitation/Emission Max

350/607 nm View spectra spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2929048

Product Specific Information

Description: The 2B8 monoclonal antibody reacts with mouse CD117, also known as c-Kit receptor, Steel factor receptor, and stem cell factor receptor. A member of the tyrosine kinase receptor family, this 145-kDa molecule is expressed by a majority of hematopoietic progenitor cells characterized in the mouse bone marrow as a small subset of cells positive for Sca-1 and Thy1 (Thy1 low) and negative for lineage markers. The interaction of the mouse c-Kit receptor and steel factor promotes the proliferation and differentiation of hematopoietic progenitor cells. CD117 is also expressed by mast cells and plays a role in signaling and activation of these cells.

Applications Reported: This 2B8 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 2B8 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This may be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Brilliant Ultra Violet™ 615 (BUV615) is a tandem dye that emits at 615 nm and is intended for use on cytometers equipped with an ultraviolet (355 nm) laser. Please make sure that your instrument is capable of detecting this fluorochrome.

When using two or more Super Bright, Brilliant Violet™, Brilliant Ultra Violet™, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer or Brilliant Stain Buffer for more information.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone-specific performance should be determined empirically.

Excitation: 350 nm; Emission: 615 nm; Laser: Ultraviolet Laser.

BRILLIANT ULTRA VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by Sirigen™.

Target Information

KIT (c-KIT) is a proto-oncogene and a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). KIT was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. KIT together with its ligand regulates growth and activation of a variety of hemopoietic and non-hemopoietic cells. Mutations in KIT are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism. Recently, deregulation of the KIT receptor TK by the prevalent activation loop mutation D816V has served as a focal point in therapeutic strategies aimed at curbing neoplastic mast cell growth. c-Kit is expressed in hematopoietic stem cells, germ cells, mast cells and gastrointestinal tract cajal cells. Upon binding of its ligand stem cell factor (SCF), c-kit dimerizes, resulting in receptor activation and autophosphorylation of various tyrosine residues including tyrosine 703 located on the cytoplasmic domain of the receptor. This modification allows docking of Grb2 and activation of the Ras/ERK signaling pathway. SCF/c-kit can activate multiple downstream signaling pathways including PI3K, PLC-gamma and JAK/STAT. c-kit receptor activation is essential for hematopoiesis, stem cell maintenance and gametogenesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: belly-spot; c-kit; c-kit proto-oncogene protein; CD117; ckit; dominant spotting; Dominant white spotting; Mast/stem cell growth factor receptor Kit; Proto-oncogene c-Kit; proto-oncogene tyrosine-protein kinase Kit; SCFR; spotted sterile male; Steel Factor Receptor; Tyrosine-protein kinase Kit

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Gene Aliases: Bs; c-KIT; CD117; Fdc; Gsfsco1; Gsfsco5; Gsfsow3; KIT; MGF; SCO1; SCO5; Sl; SOW3; Ssm; Tr-kit; W

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UniProt ID: (Pig) Q2HWD6, (Mouse) P05532

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Entrez Gene ID: (Pig) 396810, (Mouse) 16590

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Function(s)
transmembrane receptor protein tyrosine kinase activity ATP binding cytokine binding metal ion binding nucleotide binding protease binding protein kinase activity protein tyrosine kinase activity receptor signaling protein tyrosine kinase activity stem cell factor receptor activity protein binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups protein homodimerization activity transmembrane signal receptor
Process(es)
activation of MAPK activity ovarian follicle development immature B cell differentiation dendritic cell cytokine production inflammatory response spermatogenesis regulation of cell shape peptidyl-tyrosine phosphorylation cytokine-mediated signaling pathway lamellipodium assembly T cell differentiation erythrocyte differentiation melanocyte differentiation actin cytoskeleton reorganization mast cell cytokine production embryonic hemopoiesis megakaryocyte development Fc receptor signaling pathway Kit signaling pathway erythropoietin-mediated signaling pathway mast cell degranulation pigmentation digestive tract development stem cell differentiation detection of mechanical stimulus involved in sensory perception of sound cell chemotaxis mast cell differentiation melanocyte migration melanocyte adhesion myeloid progenitor cell differentiation lymphoid progenitor cell differentiation mast cell chemotaxis myeloid leukocyte differentiation protein phosphorylation glycosphingolipid metabolic process chemotaxis transmembrane receptor protein tyrosine kinase signaling pathway germ cell development spermatid development positive regulation of cell proliferation germ cell migration visual learning response to radiation positive regulation of gene expression phosphorylation hemopoiesis cell differentiation positive regulation of cell migration positive regulation of pseudopodium assembly somatic stem cell population maintenance ectopic germ cell programmed cell death intracellular signal transduction positive regulation of tyrosine phosphorylation of Stat1 protein positive regulation of tyrosine phosphorylation of Stat3 protein positive regulation of tyrosine phosphorylation of Stat5 protein negative regulation of programmed cell death positive regulation of MAP kinase activity positive regulation of MAPK cascade positive regulation of Notch signaling pathway positive regulation of JAK-STAT cascade protein autophosphorylation developmental pigmentation regulation of developmental pigmentation somatic stem cell division positive regulation of long-term neuronal synaptic plasticity epithelial cell proliferation positive regulation of sequence-specific DNA binding transcription factor activity cellular response to thyroid hormone stimulus
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