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Invitrogen
4-Hydroxy-2-hexenal Monoclonal Antibody (5C11.1), FITC
FIGURE: 1 / 1
4-Hydroxy-2-hexenal Antibody (MA5-45858) in DB
Dot blot analysis using 4-Hydroxy-2-hexenal. Samples were incubated with 4-Hydroxy-2-hexenal monoclonal antibody (Product # MA5-45858) at 1:1,000 for 2 hours at RT, followed by Goat Anti-Mouse IgG:HRP at 1:3,000 for 1 hour at RT. The quantities of protein spotted on each panel are as shown.
Product Details
MA5-45858
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chemical
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
5C11.1
Immunogen
Synthetic 4-Hydroxy-2-hexenal modified Keyhole Limpet Hemocyanin (KLH)
Conjugate
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
9.1mM sodium bicarbonate/PBS, pH 7.4, with 581mM DMSO, 123mM ethanolamine
Contains
no preservative
Storage conditions
4° C, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2932312
Product Specific Information
A 1:1,000 dilution of MA5-45858 was sufficient for detection of 4-Hydroxy-2-hexenal in 1 µg of 4-Hydroxy-2-hexenal-BSA conjugate by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.|Specific for 4-Hydroxy-2-hexenal. Slight cross-reactivity with 4-Hydroxynonenal. Does not cross-react with Acrolein, Crotonaldehyde, Hexanoyl Lysine, Malondialdehyde, or Methylglyoxal modified proteins.
Target Information
4-Hydroxynonenal, or 4-hydroxy-2-nonenal or 4-HNE or HNE, (C9H16O2), is an alpha,beta-unsaturated hydroxyalkenal that is produced by lipid peroxidation in cells. 4-HNE is the primary alpha,beta-unsaturated hydroxyalkenal formed in this process.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 4-HHE; 4-hydroxy Hexenal; 4-Hydroxy-2-hexenal; 4-Hydroxy-2-hexenal-modified; HHE; MG; MG-modified protein
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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