Invitrogen

CD366 (TIM3) Monoclonal Antibody (F38-2E2), Brilliant Violet™ 786, eBioscience™

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Datasheet
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Datasheet
Protocols
Questions & Answers

Product Details

417-3109-42

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

5 µL (1.0 µg)/test
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Brilliant Violet™ 786, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

F38-2E2

Conjugate

Brilliant Violet™ 786 Brilliant Violet™ 786 Brilliant Violet™ 786

Excitation/Emission Max

406/788 nm View spectra spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2925738

Product Specific Information

Description: This F38-2E2 monoclonal antibody reacts with human CD366, also known as T cell immunoglobulin and mucin domain-containing protein 3 (TIM3) or HAVCR2. This cell surface receptor is expressed on activated CD4+ T cell subsets (e.g. Th1, Th17, and Treg), CD8+ T cells, monocytes, dendritic cells, and mast cells. Due to alternative splicing, CD366 exists as membrane-bound and soluble forms. Galectin-9 has been identified as the ligand for CD366. In humans, this receptor negatively regulates CD4+ T cells, influencing the secretion of some Th1- and Th17-related cytokines. CD366 has also been implicated in tolerance, autoimmune disease (e.g. multiple sclerosis), and HIV infection.

Applications Reported: This F38-2E2 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This F38-2E2 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (1.0 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Brilliant Violet™ 786 (BV786) is a tandem dye that emits at 786 nm and is intended for use on cytometers equipped with a violet (405 nm) laser. Please make sure that your instrument is capable of detecting this fluorochrome.

When using two or more Super Bright, Brilliant Violet™, Brilliant Ultra Violet™, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer or Brilliant Stain Buffer for more information.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone-specific performance should be determined empirically.

Our internal testing suggests that Brilliant Violet™ 786 (BV786) is not compatible with methanol-based fixation.

Excitation: 407 nm; Emission: 786 nm; Laser: Violet Laser.

BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by Sirigen™.

Target Information

TIM3 (Hepatitis A virus cellular receptor 2, HAVCR2, T-cell immunoglobulin, mucin-dmain containing-3) is a 281 amino acid long, Type-1 Th1- specific cell surface glycoprotein expressed on terminally differentiated CD4+Th1 and CD8+Tc1 cells. TIM3 consists of an IgV-like domain, a mucin-like domain in the extracellular region, and a conserved Tyrosine phosphorylation motif in the cytoplasmic region. TIM3 is involved in macrophage activation and induction of autoimmune diseases. Further, TIM3 down-regulates aggressive Th1-mediated immune responses and facilitates in the development of immune tolerance. Pathological significance of TIM3 has been attributed to Experimental autoimmune encephalomyelitis (EAE), a Th-1 dependent autoimmune disease, and also enhances the severity of experimental autoimmune encephalomyelitis in mice.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CD366; FLJ14428; HAVcr-2; Hepatitis A virus cellular receptor 2; kidney injury molecule-3; sCD366; soluble CD366; soluble TIM 3; T cell immunoglobulin mucin 3; T cell immunoglobulin mucin-3; T-cell immunoglobulin and mucin domain-containing protein 3; T-cell immunoglobulin mucin family member 3; T-cell immunoglobulin mucin receptor 3; T-cell membrane protein 3; TIM-3; TIMD-3

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Gene Aliases: CD366; HAVcr-2; HAVCR2; KIM-3; Tim-3; TIM3; TIMD-3; TIMD3

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UniProt ID: (Human) Q8TDQ0

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Entrez Gene ID: (Human) 84868

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Function(s)
protein binding metal ion binding immunoglobulin receptor superfamily
Process(es)
adaptive immune response macrophage activation involved in immune response natural killer cell tolerance induction regulation of tolerance induction dependent upon immune response negative regulation of T-helper 1 type immune response negative regulation of natural killer cell mediated cytotoxicity directed against tumor cell target inflammatory response negative regulation of gene expression negative regulation of myeloid dendritic cell activation negative regulation of NF-kappaB transcription factor activity negative regulation of interferon-alpha production negative regulation of interferon-gamma production negative regulation of interleukin-2 production negative regulation of interleukin-3 production negative regulation of interleukin-6 production negative regulation of tumor necrosis factor production positive regulation of chemokine production positive regulation of interferon-gamma production positive regulation of interleukin-1 production positive regulation of interleukin-4 production negative regulation of natural killer cell activation toll-like receptor 3 signaling pathway toll-like receptor 7 signaling pathway toll-like receptor 9 signaling pathway positive regulation of T cell proliferation negative regulation of T cell proliferation positive regulation of macrophage activation innate immune response defense response to Gram-positive bacterium maternal process involved in female pregnancy positive regulation of ERK1 and ERK2 cascade cellular response to lipopolysaccharide negative regulation of granulocyte colony-stimulating factor production negative regulation of defense response to bacterium positive regulation of defense response to bacterium positive regulation of NIK/NF-kappaB signaling positive regulation of tumor necrosis factor secretion negative regulation of immunological synapse formation negative regulation of T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell
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