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Invitrogen
IgM Monoclonal Antibody (SA-DA4), Brilliant Violet™ 786, eBioscience™
FIGURE: 1 / 1
IgM Antibody (417-9998-42) in Flow
Normal human peripheral blood cells were stained with CD19 Monoclonal Antibody, FITC (Product # 11-0199-42) and Mouse IgG1 kappa Isotype Control, Brilliant Violet 786 (Product # 417-4714-81) (left) or IgM Monoclonal Antibody, Brilliant Violet 786 (right). Viable cells in the lymphocyte gate were used for analysis, as determined by 7-AAD (Product # 00-6993-50).
Product Details
417-9998-42
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
SA-DA4
Conjugate
Excitation/Emission Max
406/788 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Wet ice
RRID
AB_3074152
Product Specific Information
Description: The SA-DA4 monoclonal antibody reacts with the mu heavy chain of human IgM. It does not react with other classes of human immunoglobulin including IgD, IgG, or IgA. IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of mature B cells.
Our testing has revealed variations in staining of the non-B cell population that is predicted to be non-specific. This variation can be attributed to both donor variability and the anticoagulant used during blood draw. Therefore, we recommend using sodium citrate as the anticoagulant to minimize non-specific staining by this antibody.
Applications Reported: This SA-DA4 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This SA-DA4 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Brilliant Violet™ 786 (BV786) is a tandem dye that emits at 786 nm and is intended for use on cytometers equipped with a violet (405 nm) laser. Please make sure that your instrument is capable of detecting this fluorochrome.
When using two or more Super Bright, Brilliant Violet™, Brilliant Ultra Violet™, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer or Brilliant Stain Buffer for more information.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone-specific performance should be determined empirically.
Our internal testing suggests that Brilliant Violet™ 786 (BV786) is not compatible with methanol-based fixation.
Excitation: 407 nm; Emission: 786 nm; Laser: Violet Laser.
BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by Sirigen™.
Target Information
IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
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Bioinformatics
Gene Aliases: AGM1; MU; VH
Entrez Gene ID: (Human) 3507
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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