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Invitrogen
CD150 Monoclonal Antibody (mShad150), PerCP-eFluor™ 710, eBioscience™
Advanced Verification
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 7
CD150 Antibody (46-1502-82) in Flow
Staining of C57BL/6 splenocytes with Anti-Human/Mouse CD45R (B220) eFluor® 450 (Product # 48-0452-82) and 0.06 µg of Rat IgG2b K Isotype Control PerCP-eFluor® 710 (Product # 46-4031-82) (left) or 0.06 µg of Anti-Mouse CD150 PerCP-eFluor® 710 (right). Total viable cells were used for analysis.
Product Details
46-1502-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rat
/ IgG2b, lambda
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
mShad150
Conjugate
Excitation/Emission Max
482/708 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2016699
Product Specific Information
Description: The mShad150 monoclonal antibody reacts with mouse CD150, an ~70 kDa transmembrane glycoprotein also known as Signaling Lymphocyte Activation Molecule (SLAM). CD150 is expressed by T cells, in particular Th1, and B cells; this expression is rapidly upregulated upon activation. Immature thymocytes and dendritic cells also express this antigen. Signaling through SLAM in T cells induces proliferation and augmentation of the interferon-gamma response. Furthermore, SLAM is thought to play a role in adhesion between the T cell and antigen-presenting cell. The mShad150 antibody has been reported to also stain the CD150+CD244-CD48-CD41- population of pluripotent hematopoietic stem cells.
Applications Reported: This mShad150 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This mShad150 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative.
Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols.
Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD150 (SLAM, Signaling Lymphocyte Activation Molecule) belongs to the immunoglobulin gene superfamily, and is involved in T cell stimulation. SLAM is constitutively expressed on peripheral blood memory T cells, T cell clones, immature thymocytes, and a proportion of B cells, and is rapidly induced on naive T cells after activation. Activated B cells express the membrane bound form, the soluble form and the cytoplasmic isoforms of SLAM. It is suggested that signalling through homophilic SLAM-SLAM binding during B to B and B to T cell interactions enhances the expansion and differentiation of activated B cells. Reports suggest that the extracellular domain of CD150 is the receptor for the measles virus and acts as a co-activator on T cells and B cells. Moreover, CD150 is thought to interact with SH2D1A and with PTPN11 via its cytoplasmic domain. Mutations CD150 gene may be associated with X-linked lympho-proliferative disease (XLP).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD150; CD150 antigen; signaling lymphocyte activation molecule; Signaling lymphocytic activation molecule; SLAM family member 1
Gene Aliases: 4933415F16; AA177906; CD150; CDw150; ESTM51; IPO-3; Slam; Slamf1
UniProt ID: (Mouse) Q9QUM4
Entrez Gene ID: (Mouse) 27218
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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