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Invitrogen
Galectin 9 Monoclonal Antibody (RG9-35), PerCP-eFluor™ 710, eBioscience™
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 2
Galectin 9 Antibody (46-9211-82) in Flow
Mouse bone marrow cells were surface stained with CD45R (B220) Monoclonal Antibody, eFluor 450 (Product # 48-0452-82). Cells were then stained intracellularly, using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol, with 0.03 µg of Rat IgG2a kappa Isotype Control, PerCP-eFluor 710 (Product # 46-4321-82) (left) or 0.03 µg of Galectin 9 Monoclonal Antibody, PerCP-eFluor 710 (Product # 46-9211-82) (right). Cells in the lymphocyte gate were used for analysis.
Product Details
46-9211-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rat
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
RG9-35
Conjugate
Excitation/Emission Max
482/708 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2735045
Product Specific Information
Description: This RG9-35 monoclonal antibody recognizes mouse Galectin 9, a member of the S-type lectin family. Galectin 9 is widely expressed within a majority of hematopoietic cells with the particularly high expression on CD45R(B220)-intermediate pre-B cells.
Applications Reported: This RG9-35 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This RG9-35 antibody has been tested by flow cytometric analysis of mouse bone marrow cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This may be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.
Target Information
The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. The protein encoded by this gene is an S-type lectin. It is overexpressed in Hodgkin's disease tissue and might participate in the interaction between the H and RS cells with their surrounding cells and might thus play a role in the pathogenesis of this disease and/or its associated immunodeficiency. Multiple alternatively spliced transcript variants have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Gal-9; Gal9; Galectin-9; Galectin9; HOM HD 21; lectin, galactose binding, soluble 5
Gene Aliases: AA407335; AI194909; AI265545; gal-9; galectin-9; LGALS35; Lgals5; Lgals9
UniProt ID: (Mouse) O08573
Entrez Gene ID: (Mouse) 16859
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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