Invitrogen

CD3 Monoclonal Antibody (SK7), APC-eFluor™ 780, eBioscience™

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This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

20 References

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CD3 Antibody (47-0036-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD19 eFluor® 450 (Product # 48-0199-42) and Mouse IgG1 K Isotype Control APC-eFluor® 780 (Product # 47-4714-82) (left) or Anti-Human CD3 APC-eFluor® 780 (right). Cells in the lymphocyte gate were used for analysis.

Product Image Gallery

CD3 Antibody — Staining of human peripheral blood cells. As expected based on known relative expression patterns, CD3 clone SK7 stains a subset of lymphocytes (T cells) and does not stain monocytes and granulocytes (middle plot). Additional analysis of lymphocytes shows that CD3 clone SK7 does not stain any CD19+ B cells (right plot). Details: Normal human whole blood was surface stained with CD3 (clone SK7) and CD19 (clone HIB19). After staining, red blood cells were lysed using 1-step Fix/Lyse Buffer. Cells in the lymphocyte (purple histogram), monocyte (orange histogram), or granulocyte (blue histogram) gates were used to compare CD3 staining (middle plot). Cells in the lymphocyte gate were used to compare CD3 and CD19 staining (right plot). {RE}

Product Details

47-0036-42

Applications

Tested Dilution

Publications

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Flow Cytometry (Flow)

5 µL (0.25 µg)/test

View 19 publications 19 publications

Product Specifications

Species Reactivity

Chimpanzee, Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC-eFluor™ 780, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

SK7

Conjugate

APC-eFluor™ 780 APC-eFluor™ 780 APC-eFluor™ 780

Excitation/Emission Max

756/785 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10717514

Product Specific Information

Description: The SK7 monoclonal antibody reacts with human and chimpanzee CD3e, a 20 kDa subunit of the TCR complex. Along with the other CD3 subunits gamma and delta, the epsilon chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. The SK7 and UCHT1 monoclonal antibodies cross-block binding, suggesting recognition of overlapping epitope. In contrast, clones OKT3 and SK7 see different epitopes.

The antibody SK7 recognizes chimpanzee CD3.

Applications Reported: This SK7 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This SK7 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Light sensitivity: This tandem is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The CD3 subunit complex which is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. The genes encoding the CD3 epsilon, gamma and delta polypeptides are located on chromosome 11. Defects in the CD3 gene are associated with CD3 immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition).

European journal of immunology

Cossarizza A,Chang HD,Radbruch A,Abrignani S,Addo R,Akdis M,Andrä I,Andreata F,Annunziato F,Arranz E,Bacher P,Bari S,Barnaba V,Barros-Martins J,Baumjohann D,Beccaria CG,Bernardo D,Boardman DA,Borger J,Böttcher C,Brockmann L,Burns M,Busch DH,Cameron G,Cammarata I,Cassotta A,Chang Y,Chirdo FG,Christakou E,Čičin-Šain L,Cook L,Corbett AJ,Cornelis R,Cosmi L,Davey MS,De Biasi S,De Simone G,Del Zotto G,Delacher M,Di Rosa F,Di Santo J,Diefenbach A,Dong J,Dörner T,Dress RJ,Dutertre CA,Eckle SBG,Eede P,Evrard M,Falk CS,Feuerer M,Fillatreau S,Fiz-Lopez A,Follo M,Foulds GA,Fröbel J,Gagliani N,Galletti G,Gangaev A,Garbi N,Garrote JA,Geginat J,Gherardin NA,Gibellini L,Ginhoux F,Godfrey DI,Gruarin P,Haftmann C,Hansmann L,Harpur CM,Hayday AC,Heine G,Hernández DC,Herrmann M,Hoelsken O,Huang Q,Huber S,Huber JE,Huehn J,Hundemer M,Hwang WYK,Iannacone M,Ivison SM,Jäck HM,Jani PK,Keller B,Kessler N,Ketelaars S,Knop L,Knopf J,Koay HF,Kobow K,Kriegsmann K,Kristyanto H,Krueger A,Kuehne JF,Kunze-Schumacher H,Kvistborg P,Kwok I,Latorre D,Lenz D,Levings MK,Lino AC,Liotta F,Long HM,Lugli E,MacDonald KN,Maggi L,Maini MK,Mair F,Manta C,Manz RA,Mashreghi MF,Mazzoni A,McCluskey J,Mei HE,Melchers F,Melzer S,Mielenz D,Monin L,Moretta L,Multhoff G,Muñoz LE,Muñoz-Ruiz M,Muscate F,Natalini A,Neumann K,Ng LG,Niedobitek A,Niemz J,Almeida LN,Notarbartolo S,Ostendorf L,Pallett LJ,Patel AA,Percin GI,Peruzzi G,Pinti M,Pockley AG,Pracht K,Prinz I,Pujol-Autonell I,Pulvirenti N,Quatrini L,Quinn KM,Radbruch H,Rhys H,Rodrigo MB,Romagnani C,Saggau C,Sakaguchi S,Sallusto F,Sanderink L,Sandrock I,Schauer C,Scheffold A,Scherer HU,Schiemann M,Schildberg FA,Schober K,Schoen J,Schuh W,Schüler T,Schulz AR,Schulz S,Schulze J,Simonetti S,Singh J,Sitnik KM,Stark R,Starossom S,Stehle C,Szelinski F,Tan L,Tarnok A,Tornack J,Tree TIM,van Beek JJP,van de Veen W,van Gisbergen K,Vasco C,Verheyden NA,von Borstel A,Ward-Hartstonge KA,Warnatz K,Waskow C,Wiedemann A,Wilharm A,Wing J,Wirz O,Wittner J,Yang JHM,Yang J

47-0036-42 was used in Flow cytometry/Cell sorting to provide the key aspects to consider when performing flow cytometry experiments.

Wed Dec 01 00:00:00 EST 2021

Multipeptide stimulated PBMCs generate T_EM/T_CM for adoptive cell therapy in multiple myeloma.

Oncotarget

Vardam-Kaur T,Pathangey LB,McCormick DJ,Bergsagel PL,Cohen PA,Gendler SJ

47-0036-42 was used in Flow Cytometry to evaluate the possibility of adoptive cell transfer (ACT) as a immunotherapeutic strategy for Multiple Myeloma (MM) patients, by generating ex vivo T cells from peripheral blood mononuclear cells (PBMCs) isolated from MM patients by employing our previously devised protocols.

Tue Sep 28 00:00:00 EDT 2021

Droplet-based mRNA sequencing of fixed and permeabilized cells by CLInt-seq allows for antigen-specific TCR cloning.

Proceedings of the National Academy of Sciences of the United States of America

Nesterenko PA,McLaughlin J,Cheng D,Bangayan NJ,Burton Sojo G,Seet CS,Qin Y,Mao Z,Obusan MB,Phillips JW,Witte ON

47-0036-42 was used in Flow cytometry/Cell sorting to describe the use of mRNA sequencing via cross-linker regulated intracellular phenotype (CLInt-Seq) for efficient recovery of antigen-specific TCRs in cells stained for combinations of intracellular proteins such as cytokines or transcription factors.

Tue Jan 19 00:00:00 EST 2021

Th1 responses in vivo require cell-specific provision of OX40L dictated by environmental cues.

Nature communications

Gajdasik DW,Gaspal F,Halford EE,Fiancette R,Dutton EE,Willis C,Rückert T,Romagnani C,Gerard A,Bevington SL,MacDonald AS,Botto M,Vyse T,Withers DR

47-0036 was used in Flow cytometry/Cell sorting to reveal tissue specific compartmentalisation of the cellular provision of OX40L and define a mechanism controlling DC expression of OX40L in vivo.

Thu Jul 09 00:00:00 EDT 2020

Circulating CD56^bright NK cells inversely correlate with survival of melanoma patients.

Scientific reports

de Jonge K,Ebering A,Nassiri S,Maby-El Hajjami H,Ouertatani-Sakouhi H,Baumgaertner P,Speiser DE

47-0036 was used in Flow cytometry/Cell sorting to characterise natural killer cells from peripheral blood obtained from late stage (III/IV) melanoma patients.

Thu Mar 14 00:00:00 EDT 2019

A transcriptionally and functionally distinct PD-1⁺ CD8⁺ T cell pool with predictive potential in non-small-cell lung cancer treated with PD-1 blockade.

Nature medicine

Thommen DS,Koelzer VH,Herzig P,Roller A,Trefny M,Dimeloe S,Kiialainen A,Hanhart J,Schill C,Hess C,Savic Prince S,Wiese M,Lardinois D,Ho PC,Klein C,Karanikas V,Mertz KD,Schumacher TN,Zippelius A

47-0036 was used in Flow cytometry/Cell sorting to compare transcriptional, metabolic and functional signatures of intratumoral CD8+ T lymphocyte populations with high, intermediate and no PD-1 expression from non-small-cell lung cancer patients.

Sun Jul 01 00:00:00 EDT 2018

ATP Release from Chemotherapy-Treated Dying Leukemia Cells Elicits an Immune Suppressive Effect by Increasing Regulatory T Cells and Tolerogenic Dendritic Cells.

Frontiers in immunology

Lecciso M,Ocadlikova D,Sangaletti S,Trabanelli S,De Marchi E,Orioli E,Pegoraro A,Portararo P,Jandus C,Bontadini A,Redavid A,Salvestrini V,Romero P,Colombo MP,Di Virgilio F,Cavo M,Adinolfi E,Curti A

47-0036 was used in Flow cytometry/Cell sorting to study the effect of ATP release in acute myeloid lymphoma on the generation of an immune suppressive microenvironment.

Thu Mar 31 00:00:00 EDT 2022

Increased IL-15 Production and Accumulation of Highly Differentiated CD8⁺ Effector/Memory T Cells in the Bone Marrow of Persons with Cytomegalovirus.

Frontiers in immunology

Pangrazzi L,Naismith E,Meryk A,Keller M,Jenewein B,Trieb K,Grubeck-Loebenstein B

47-0036 was used in Flow cytometry/Cell sorting to imply that a bone marrow environment rich in IL-15 may be important in the maintenance of differentiated CD8(+) T cells generated after cytomegalovirus infection.

Sun Apr 10 00:00:00 EDT 2022

Dominant immunosuppression of dendritic cell function by prostate-cancer-derived exosomes.

Journal of extracellular vesicles

Salimu J,Webber J,Gurney M,Al-Taei S,Clayton A,Tabi Z

47-0036 was used in Flow cytometry/Cell sorting to identify exosomal prostaglandin E2 as a driver of CD73 induction, as prostaglandin E2 receptor inhibition significantly reduced exosome-dependent CD73 induction.

Wed Sep 30 00:00:00 EDT 2020

Surrogate in vitro activation of innate immunity synergizes with interleukin-7 to unleash rapid antigen-driven outgrowth of CD4+ and CD8+ human peripheral blood T-cells naturally recognizing MUC1, HER2/neu and other tumor-associated antigens.

Oncotarget

Pathangey LB,McCurry DB,Gendler SJ,Dominguez AL,Gorman JE,Pathangey G,Mihalik LA,Dang Y,Disis ML,Cohen PA

47-0036 was used in Flow cytometry/Cell sorting to create a mechanistically rational culture sequence, enabling rapid preparation of T-cells recognizing tumor-associated antigens expressed by the majority of human cancer.

Tue Feb 14 00:00:00 EST 2017

Prostaglandin E₂-mediated adenosinergic effects on CD14⁺ cells: Self-amplifying immunosuppression in cancer.

Oncoimmunology

Al-Taei S,Salimu J,Spary LK,Clayton A,Lester JF,Tabi Z

47-0036 was used in Flow cytometry/Cell sorting to show that CD73 expression can be induced by PGE2, cAMP or adenosine on human CD14+ cells.

Tue Jan 12 00:00:00 EST 2021

Eomeshi NK Cells in Human Liver Are Long-Lived and Do Not Recirculate but Can Be Replenished from the Circulation.

Journal of immunology (Baltimore, Md. : 1950)

Cuff AO,Robertson FP,Stegmann KA,Pallett LJ,Maini MK,Davidson BR,Male V

47-0036 was used in Flow cytometry/Cell sorting to study the molecular signal that characterises an Eomeshi population of natural killer cells in the human liver that is not present in the blood.

Thu Dec 01 00:00:00 EST 2016

Platelet-Derived Ectosomes Reduce NK Cell Function.

Journal of immunology (Baltimore, Md. : 1950)

Sadallah S,Schmied L,Eken C,Charoudeh HN,Amicarella F,Schifferli JA

47-0036 was used in Flow cytometry/Cell sorting to study whether platelet ectosomes modify natural killer cell function.

Thu Sep 01 00:00:00 EDT 2016

Human and Murine Hematopoietic Stem Cell Aging Is Associated with Functional Impairments and Intrinsic Megakaryocytic/Erythroid Bias.

PloS one

Rundberg Nilsson A,Soneji S,Adolfsson S,Bryder D,Pronk CJ

47-0036 was used in Flow cytometry/Cell sorting to support the notion that age-related changes also in human hematopoiesis involve the hematopoietic stem cell pool.

Tue Aug 08 00:00:00 EDT 2017

Switching CAR T cells on and off: a novel modular platform for retargeting of T cells to AML blasts.

Blood cancer journal

Cartellieri M,Feldmann A,Koristka S,Arndt C,Loff S,Ehninger A,von Bonin M,Bejestani EP,Ehninger G,Bachmann MP

47-0036 was used in Flow cytometry/Cell sorting to describe a novel modular universal platform for retargeting of T cells to AML blasts, via switching CD19-specific chimeric antigen receptor engineered T cells on or off.

Fri Aug 12 00:00:00 EDT 2016

Impaired survival of regulatory T cells in pulmonary sarcoidosis.

Respiratory research

Broos CE,van Nimwegen M,Kleinjan A,ten Berge B,Muskens F,in 't Veen JC,Annema JT,Lambrecht BN,Hoogsteden HC,Hendriks RW,Kool M,van den Blink B

47-0036 was used in Flow cytometry/Cell sorting to investigate the role of impaired Treg function in sarcoidosis.

Wed Sep 16 00:00:00 EDT 2015

CALGB 150905 (Alliance): rituximab broadens the antilymphoma response by activating unlicensed NK cells.

Cancer immunology research

Du J,Lopez-Verges S,Pitcher BN,Johnson J,Jung SH,Zhou L,Hsu K,Czuczman MS,Cheson B,Kaplan L,Lanier LL,Venstrom JM

47-0036 was used in Flow cytometry/Cell sorting to investgate the rules determining NK-cell responsiveness to mAb therapies.

Mon Sep 01 00:00:00 EDT 2014

KIR/HLA interactions negatively affect rituximab- but not GA101 (obinutuzumab)-induced antibody-dependent cellular cytotoxicity.

Journal of immunology (Baltimore, Md. : 1950)

Terszowski G,Klein C,Stern M

47-0036 was used in Flow cytometry/Cell sorting to show that modification of the Fc fragment to enhance ADCC can be an effective strategy to augment the efficacy of therapeutic mAbs by recruiting NK cells irrespective of their inhibitory KIR expression.

Sun Jun 15 00:00:00 EDT 2014

A novel ex vivo isolation and expansion procedure for chimeric antigen receptor engrafted human T cells.

PloS one

Cartellieri M,Koristka S,Arndt C,Feldmann A,Stamova S,von Bonin M,Töpfer K,Krüger T,Geib M,Michalk I,Temme A,Bornhäuser M,Lindemann D,Ehninger G,Bachmann MP

47-0036 was used in Flow cytometry/Cell sorting to assess the effectiveness of a novel peptide epitope to trigger the expansion of specific populations of T cells.

Thu Jan 29 00:00:00 EST 2015

A monoclonal antibody selection for immunohistochemical examination of lymphoid tissues from non-human primates.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society

Kap YS,van Meurs M,van Driel N,Koopman G,Melief MJ,Brok HP,Laman JD,'t Hart BA

Tue Dec 01 00:00:00 EST 2009

Bioinformatics

Protein Aliases: CD247; CD247 antigen, zeta subunit; CD3 antigen, delta subunit; CD3 delta; CD3 TCR complex; CD3-epsilon; CD3d; CD3d antigen, delta polypeptide (TiT3 complex); CD3d molecule, delta (CD3-TCR complex); CD3e; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3g; CD3g antigen, gamma polypeptide (TiT3 complex); CD3g molecule, epsilon (CD3-TCR complex); CD3g molecule, gamma (CD3-TCR complex); CD3Z antigen, zeta polypeptide (TiT3 complex); CD3zeta chain; FLJ18683; Leu-4; OKT3, delta chain; T cell antigen receptor complex epsilon subunit of T3; T-cell antigen receptor complex, epsilon subunit of T3; T-cell antigen receptor complex, gamma subunit of T3; T-cell antigen receptor complex, zeta subunit of CD3; T-cell receptor T3 delta chain; T-cell receptor T3 gamma chain; T-cell receptor T3 zeta chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 gamma chain; T-cell surface glycoprotein CD3 zeta chain; T3/TCR complex; TCR zeta chain

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Gene Aliases: CD247; CD3-DELTA; CD3-GAMMA; CD3-ZETA; CD3D; CD3E; CD3G; CD3H; CD3Q; CD3Z; IMD17; IMD18; IMD19; IMD25; T3D; T3E; T3G; T3Z; TCRE; TCRZ

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UniProt ID: (Human) P20963, (Human) P04234, (Human) P07766, (Human) P09693

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Entrez Gene ID: (Human) 919, (Human) 915, (Human) 916, (Human) 917

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Function(s)

transmembrane signaling receptor activity protein binding identical protein binding protein homodimerization activity transcription coactivator activity protein heterodimerization activity receptor signaling protein activity SH3 domain binding protein kinase binding receptor signaling complex scaffold activity T cell receptor binding defense/immunity protein immunoglobulin receptor superfamily

Process(es)

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CD3 Monoclonal Antibody (SK7), APC-eFluor™ 780, eBioscience™

CD3 Antibody (47-0036-42) in Flow

Product Details

47-0036-42

Immunohistochemistry (Frozen) (IHC (F))

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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