Invitrogen

CD23 Monoclonal Antibody (B3B4), eFluor™ 450, eBioscience™

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CD23 Antibody (48-0232-82) in Flow

Staining of BALB/c splenocytes with Anti-Human/Mouse CD45R (B220) APC (Product # 17-0452-82) and 0.25 µg of Rat IgG2a K Isotype Control eFluor® 450 (Product # 48-4321-82) (left) or 0.25 µg of Anti-Mouse CD23 eFluor® 450 (right). Total viable cells were used for analysis.

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Product Details

48-0232-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.5 µg/test

View 15 publications 15 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), eFluor™ 450, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

B3B4

Conjugate

eFluor™ 450 eFluor™ 450 eFluor™ 450

Excitation/Emission Max

405/445 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11220067

Product Specific Information

Description: The B3B4 monoclonal antibody reacts with mouse CD23, a 45 kDa type II transmembrane glycoprotein. CD23 is expressed on resting conventional B cells, and its expression is modulated upon B-cell activation. B-1 cell lineage (CD5+ B cells) does not express CD23. Soluble forms of the antigen have been reported to be biologically active. CD23 is a low affinity receptor for IgE and is thought to play a role in the regulation of IgE response and B-cell activation. CD21 is thought to bind to CD23.

Applications Reported: This B3B4 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This B3B4 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD23 is a 45 kDa glycoprotein which is present on a subpopulation of freshly isolated peripheral blood and tonsil B cells and strongly expressed on EBV-transformed B lymphoblasts. The CD23 molecule is identical to the low affinity IgE receptor found on B cells. Expression of CD23 has been detected in neoplastic cells from cases of B cell chronic lymphocyctic leukaemia and some cases of centroblastic/centrocytic lymphoma. CD23 is present on a subpopulation of freshly isolated peripheral blood and tonsil B cells and strongly expressed on EBV-transformed B lymphoblasts. Functionally, CD23 is involved in B cell growth and differentiation, and IgE production. Further, CD23 has a soluble form that is a potent mitogenic factor. Diseases associated with CD23 dysfunction include chronic conjunctivitis and chronic lymphocytic leukemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Smc3 dosage regulates B cell transit through germinal centers and restricts their malignant transformation.

Nature immunology

Rivas MA,Meydan C,Chin CR,Challman MF,Kim D,Bhinder B,Kloetgen A,Viny AD,Teater MR,McNally DR,Doane AS,Béguelin W,Fernández MTC,Shen H,Wang X,Levine RL,Chen Z,Tsirigos A,Elemento O,Mason CE,Melnick AM

Published figure using CD23 monoclonal antibody (Product # 48-0232-82) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2021

Intestinal CD11b⁺ B Cells Ameliorate Colitis by Secreting Immunoglobulin A.

Frontiers in immunology

Fu Y,Wang Z,Yu B,Lin Y,Huang E,Liu R,Zhao C,Lu M,Xu W,Liu H,Liu Y,Wang L,Chu Y

48-0232-82 was used in Flow Cytometry to demonstrate that CD11b+ B cells are essential intestinal suppressive immune cells and the primary source of intestinal IgA, which plays an indispensable role in maintaining intestinal homeostasis.

Fri Jan 07 00:00:00 EST 2022

Isotype Switching Converts Anti-CD40 Antagonism to Agonism to Elicit Potent Antitumor Activity.

Cancer cell

Yu X,Chan HTC,Fisher H,Penfold CA,Kim J,Inzhelevskaya T,Mockridge CI,French RR,Duriez PJ,Douglas LR,English V,Verbeek JS,White AL,Tews I,Glennie MJ,Cragg MS

Published figure using CD23 monoclonal antibody (Product # 48-0232-82) in Flow Cytometry

Mon Jun 08 00:00:00 EDT 2020

Deregulated Notch and Wnt signaling activates early-stage myeloid regeneration pathways in leukemia.

The Journal of experimental medicine

Kang YA,Pietras EM,Passegué E

Published figure using CD23 monoclonal antibody (Product # 48-0232-82) in Flow Cytometry

Mon Mar 02 00:00:00 EST 2020

Activated PI3Kδ breaches multiple B cell tolerance checkpoints and causes autoantibody production.

The Journal of experimental medicine

Lau A,Avery DT,Jackson K,Lenthall H,Volpi S,Brigden H,Russell AJ,Bier J,Reed JH,Smart JM,Cole T,Choo S,Gray PE,Berglund LJ,Hsu P,Wong M,O'Sullivan M,Boztug K,Meyts I,Uzel G,Notarangelo LD,Brink R,Goodnow CC,Tangye SG,Deenick EK

Published figure using CD23 monoclonal antibody (Product # 48-0232-82) in Flow Cytometry

Mon Feb 03 00:00:00 EST 2020

CD83 expression regulates antibody production in response to influenza A virus infection.

Virology journal

Akauliya M,Gautam A,Maharjan S,Park BK,Kim J,Kwon HJ

Published figure using CD23 monoclonal antibody (Product # 48-0232-82) in Flow Cytometry

Thu Dec 10 00:00:00 EST 2020

Estrogen Receptor Alpha Signaling Is Responsible for the Female Sex Bias in the Loss of Tolerance and Immune Cell Activation Induced by the Lupus Susceptibility Locus Sle1b.

Frontiers in immunology

Graham JH,Yoachim SD,Gould KA

Published figure using CD23 monoclonal antibody (Product # 48-0232-82) in Flow Cytometry

Tue Jun 22 00:00:00 EDT 2021

Aging Induces an Nlrp3 Inflammasome-Dependent Expansion of Adipose B Cells That Impairs Metabolic Homeostasis.

Cell metabolism

Camell CD,Günther P,Lee A,Goldberg EL,Spadaro O,Youm YH,Bartke A,Hubbard GB,Ikeno Y,Ruddle NH,Schultze J,Dixit VD

48-0232 was used in Flow cytometry/Cell sorting to study the role of ageing within alterations of adipose tissue.

Tue Dec 03 00:00:00 EST 2019

Peritoneal Cells Mediate Immune Responses and Cross-Protection Against Influenza A Virus.

Frontiers in immunology

Gautam A,Park BK,Kim TH,Akauliya M,Kim D,Maharjan S,Park J,Kim J,Lee H,Park MS,Lee Y,Kwon HJ

Published figure using CD23 monoclonal antibody (Product # 48-0232-82) in Flow Cytometry

Mon Aug 17 00:00:00 EDT 2020

Activated Peyer's patch B cells sample antigen directly from M cells in the subepithelial dome.

Nature communications

Komban RJ,Strömberg A,Biram A,Cervin J,Lebrero-Fernández C,Mabbott N,Yrlid U,Shulman Z,Bemark M,Lycke N

Published figure using CD23 monoclonal antibody (Product # 48-0232-82) in Flow Cytometry

Mon Jun 03 00:00:00 EDT 2019

Prolonged Deleterious Influences of Chemotherapeutic Agent CPT-11 on Resident Peritoneal Macrophages and B1 Cells.

Frontiers in immunology

Bai WJ,Li CG,Zhang CC,Xu LH,Zeng QZ,Hu B,Hong Z,He XH,Ouyang DY

Published figure using CD23 monoclonal antibody (Product # 48-0232-82) in Flow Cytometry

Wed Nov 20 00:00:00 EST 2019

Secreted IgM deficiency leads to increased BCR signaling that results in abnormal splenic B cell development.

Scientific reports

Tsiantoulas D,Kiss M,Bartolini-Gritti B,Bergthaler A,Mallat Z,Jumaa H,Binder CJ

48-0232 was used in Flow cytometry/Cell sorting to identify secreted IgM as negative regulators of BCR signalling and suggest that they can act as decoy receptors for self-antigens that are recognised by membrane bound BCRs.

Wed Jun 14 00:00:00 EDT 2017

Mantle cell lymphoma in cyclin D1 transgenic mice with Bim-deficient B cells.

Blood

Katz SG,Labelle JL,Meng H,Valeriano RP,Fisher JK,Sun H,Rodig SJ,Kleinstein SH,Walensky LD

Published figure using CD23 monoclonal antibody (Product # 48-0232-82) in Flow Cytometry

Thu Feb 06 00:00:00 EST 2014

Critical role of B cell lymphoma 10 in BAFF-regulated NF-κB activation and survival of anergic B cells.

Journal of immunology (Baltimore, Md. : 1950)

Yu M,Chen Y,He Y,Podd A,Fu G,Wright JA,Kleiman E,Khan WN,Wen R,Wang D

Published figure using CD23 monoclonal antibody (Product # 48-0232-82) in Flow Cytometry

Sat Dec 01 00:00:00 EST 2012

A critical role of TAK1 in B-cell receptor-mediated nuclear factor kappaB activation.

Blood

Schuman J,Chen Y,Podd A,Yu M,Liu HH,Wen R,Chen ZJ,Wang D

Published figure using CD23 monoclonal antibody (Product # 48-0232-82) in Flow Cytometry

Thu May 07 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: CD23; FC epsilon RII; Fc-epsilon-RII; FceRII; Low affinity immunoglobulin epsilon Fc receptor; low-affinity IgE receptor; Lymphocyte IgE receptor

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Gene Aliases: CD23; Fce2; Fcer2; Fcer2a; Ly-42

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UniProt ID: (Mouse) P20693

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Entrez Gene ID: (Mouse) 14128

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Function(s)

IgE binding carbohydrate binding metal ion binding membrane traffic protein

Process(es)

positive regulation of humoral immune response mediated by circulating immunoglobulin positive regulation of nitric-oxide synthase activity positive regulation of killing of cells of other organism positive regulation of nitric-oxide synthase biosynthetic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CD23 Monoclonal Antibody (B3B4), eFluor™ 450, eBioscience™

CD23 Antibody (48-0232-82) in Flow

Product Details

48-0232-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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