Invitrogen

CD71 (Transferrin Receptor) Monoclonal Antibody (R17217 (RI7 217.1.4)), eFluor™ 450, eBioscience™

CD71 (Transferrin Receptor) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 27

CD71 (Transferrin Receptor) Antibody (48-0711-82) in Flow

Staining of C57Bl/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) APC (Product # 17-0452-82) and 0.125 µg of Rat IgG2a K Isotype Control eFluor® 450 (Product # 48-4321-82) (left) or 0.125 µg of Anti-Mouse CD71 (Transferrin Receptor) eFluor® 450 (right). Cells in the large scatter population were used for analysis.

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Product Details

48-0711-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.25 µg/test

View 21 publications 21 publications

Product Specifications

Species Reactivity

Mouse

Published species

Fish, Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), eFluor™ 450, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

R17217 (RI7 217.1.4)

Conjugate

eFluor™ 450 eFluor™ 450 eFluor™ 450

Excitation/Emission Max

405/445 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2574027

Product Specific Information

Description: The R17217 monoclonal antibody reacts with mouse CD71, a 170-180 kDa type II transmembrane protein. CD71, the transferrin receptor, exists as a homodimer on the cell surface and is essential for cellular growth. CD71 is expressed by immature proliferating cells and at low levels on resting mature lymphocytes. Antigen or mitogen stimulation of T and B cells upregulates the expression of CD71. Expression level differences have been observed in different mouse strains.

Applications Reported: This R17217 (RI7 217.1.4) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This R17217 (RI7 217.1.4) antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system. Serum transferrin receptor (sTfR) is used as a means of detecting erythropoietin (EPO) misuse by athletes and as a diagnostic test for anemias resulting from a number of conditions including rheumatoid arthritis, pregnancy, irritable bowel syndrome and in HIV patients.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Perivascular localized cells commit erythropoiesis in PDGF-B-expressing solid tumors.

Cancer communications (London, England)

Hosaka K,Wang C,Zhang S,Lv X,Seki T,Zhang Y,Jing X,Wu J,Du Q,He X,Fan Y,Li X,Kondo M,Yoshihara M,Qian H,Shi L,Zhu P,Xu Y,Yang Y,Cheng T,Cao Y

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 48-0711-82) in Flow Cytometry

Thu Jun 01 00:00:00 EDT 2023

In vivo CRISPR screening reveals nutrient signaling processes underpinning CD8⁺ T cell fate decisions.

Cell

Huang H,Zhou P,Wei J,Long L,Shi H,Dhungana Y,Chapman NM,Fu G,Saravia J,Raynor JL,Liu S,Palacios G,Wang YD,Qian C,Yu J,Chi H

48-0711-82 was used in Flow Cytometry to establish that nutrient uptake and signaling are key determinants of T cell fate and shape the quantity and quality of TMEM responses.

Thu Mar 04 00:00:00 EST 2021

NSMF promotes the replication stress-induced DNA damage response for genome maintenance.

Nucleic acids research

Ju MK,Shin KJ,Lee JR,Khim KW,A Lee E,Ra JS,Kim BG,Jo HS,Yoon JH,Kim TM,Myung K,Choi JH,Kim H,Chae YC

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 48-0711-82) in Flow Cytometry

Fri Jun 04 00:00:00 EDT 2021

Engineered red blood cells carrying PCSK9 inhibitors persistently lower LDL and prevent obesity.

PloS one

Deshycka R,Sudaryo V,Huang NJ,Xie Y,Smeding LY,Choi MK,Ploegh HL,Lodish HF,Pishesha N

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 48-0711-82) in Flow Cytometry

Fri Dec 24 00:00:00 EST 2021

Evaluating the Role of the Steroid and Xenobiotic Receptor (SXR/PXR) in PCB-153 Metabolism and Protection against Associated Adverse Effects during Perinatal and Chronic Exposure in Mice.

Environmental health perspectives

Egusquiza RJ,Ambrosio ME,Wang SG,Kay KM,Zhang C,Lehmler HJ,Blumberg B

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 48-0711-82) in Flow Cytometry

Wed Apr 01 00:00:00 EDT 2020

miR‑210 enhances mesenchymal stem cell‑modulated neural precursor cell migration.

Molecular medicine reports

Wang F,Zhu J,Zheng J,Duan W,Zhou Z

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 48-0711-82) in Flow Cytometry

Mon Jun 01 00:00:00 EDT 2020

Gene-centric functional dissection of human genetic variation uncovers regulators of hematopoiesis.

eLife

Nandakumar SK,McFarland SK,Mateyka LM,Lareau CA,Ulirsch JC,Ludwig LS,Agarwal G,Engreitz JM,Przychodzen B,McConkey M,Cowley GS,Doench JG,Maciejewski JP,Ebert BL,Root DE,Sankaran VG

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 48-0711-82) in Flow Cytometry

Thu May 09 00:00:00 EDT 2019

A cryptic cycle in haematopoietic niches promotes initiation of malaria transmission and evasion of chemotherapy.

Nature communications

Lee RS,Waters AP,Brewer JM

48-0711 was used in Flow cytometry/Cell sorting to enumerate Plasmodium infected erythropoietic precursor cells using an in vivo rodent model of Plasmodium berghei.

Fri Apr 27 00:00:00 EDT 2018

TGF-β-Induced Quiescence Mediates Chemoresistance of Tumor-Propagating Cells in Squamous Cell Carcinoma.

Cell stem cell

Brown JA,Yonekubo Y,Hanson N,Sastre-Perona A,Basin A,Rytlewski JA,Dolgalev I,Meehan S,Tsirigos A,Beronja S,Schober M

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 48-0711-82) in Flow Cytometry

Thu Nov 02 00:00:00 EDT 2017

Intron 1 GATA site enhances ALAS2 expression indispensably during erythroid differentiation.

Nucleic acids research

Zhang Y,Zhang J,An W,Wan Y,Ma S,Yin J,Li X,Gao J,Yuan W,Guo Y,Engel JD,Shi L,Cheng T,Zhu X

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 48-0711-82) in Flow Cytometry

Wed Jan 25 00:00:00 EST 2017

Genetically engineered red cells expressing single domain camelid antibodies confer long-term protection against botulinum neurotoxin.

Nature communications

Huang NJ,Pishesha N,Mukherjee J,Zhang S,Deshycka R,Sudaryo V,Dong M,Shoemaker CB,Lodish HF

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 48-0711-82) in Flow Cytometry

Mon Sep 04 00:00:00 EDT 2017

Hepcidin is regulated by promoter-associated histone acetylation and HDAC3.

Nature communications

Pasricha SR,Lim PJ,Duarte TL,Casu C,Oosterhuis D,Mleczko-Sanecka K,Suciu M,Da Silva AR,Al-Hourani K,Arezes J,McHugh K,Gooding S,Frost JN,Wray K,Santos A,Porto G,Repapi E,Gray N,Draper SJ,Ashley N,Soilleux E,Olinga P,Muckenthaler MU,Hughes JR,Rivella S,Milne TA,Armitage AE,Drakesmith H

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 48-0711-82) in Flow Cytometry

Fri Sep 01 00:00:00 EDT 2017

The Poly(C) Binding Protein Pcbp2 and Its Retrotransposed Derivative Pcbp1 Are Independently Essential to Mouse Development.

Molecular and cellular biology

Ghanem LR,Kromer A,Silverman IM,Chatterji P,Traxler E,Penzo-Mendez A,Weiss MJ,Stanger BZ,Liebhaber SA

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 48-0711-82) in Flow Cytometry

Fri Jan 15 00:00:00 EST 2016

Effects of nerve growth factor and basic fibroblast growth factor dual gene modification on rat bone marrow mesenchymal stem cell differentiation into neuron-like cells in vitro.

Molecular medicine reports

Hu Y,Zhang Y,Tian K,Xun C,Wang S,Lv D

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 48-0711-82) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2016

Reprogramming mouse fibroblasts into engraftable myeloerythroid and lymphoid progenitors.

Nature communications

Cheng H,Ang HY,A El Farran C,Li P,Fang HT,Liu TM,Kong SL,Chin ML,Ling WY,Lim EK,Li H,Huber T,Loh KM,Loh YH,Lim B

48-0711 was used in Flow cytometry/Cell sorting to demonstrate how it is possible to reprogram mouse embryonic fibroblasts into engraftable blood progenitors.

Mon Nov 21 00:00:00 EST 2016

PPAR-α and glucocorticoid receptor synergize to promote erythroid progenitor self-renewal.

Nature

Lee HY,Gao X,Barrasa MI,Li H,Elmes RR,Peters LL,Lodish HF

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 48-0711-82) in Flow Cytometry

Thu Jun 25 00:00:00 EDT 2015

Genome-wide association study follow-up identifies cyclin A2 as a regulator of the transition through cytokinesis during terminal erythropoiesis.

American journal of hematology

Ludwig LS,Cho H,Wakabayashi A,Eng JC,Ulirsch JC,Fleming MD,Lodish HF,Sankaran VG

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 48-0711-82) in Flow Cytometry

Fri May 01 00:00:00 EDT 2015

Mitochondrial ATP transporter Ant2 depletion impairs erythropoiesis and B lymphopoiesis.

Cell death and differentiation

Cho J,Seo J,Lim CH,Yang L,Shiratsuchi T,Lee MH,Chowdhury RR,Kasahara H,Kim JS,Oh SP,Lee YJ,Terada N

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 48-0711-82) in Flow Cytometry

Tue Sep 01 00:00:00 EDT 2015

A Systems Approach Identifies Essential FOXO3 Functions at Key Steps of Terminal Erythropoiesis.

PLoS genetics

Liang R,Campreciós G,Kou Y,McGrath K,Nowak R,Catherman S,Bigarella CL,Rimmelé P,Zhang X,Gnanapragasam MN,Bieker JJ,Papatsenko D,Ma'ayan A,Bresnick E,Fowler V,Palis J,Ghaffari S

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 48-0711-82) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2015

Concomitant inactivation of Rb and E2f8 in hematopoietic stem cells synergizes to induce severe anemia.

Blood

Hu T,Ghazaryan S,Sy C,Wiedmeyer C,Chang V,Wu L

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 48-0711-82) in Flow Cytometry

Thu May 10 00:00:00 EDT 2012

Insulin stimulates the phosphorylation of the exocyst protein Sec8 in adipocytes.

Bioscience reports

Lyons PD,Peck GR,Kettenbach AN,Gerber SA,Roudaia L,Lienhard GE

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 48-0711-82) in Flow Cytometry

Sat Aug 01 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: CD antigen CD71; CD71; mammary tumor virus receptor 1; TR; Transferrin receptor protein 1

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Gene Aliases: 2610028K12Rik; AI195355; AI426448; AU015758; CD71; E430033M20Rik; Mtvr-1; Mtvr1; p90; TFR; TFR1; Tfrc; TR; Trfr

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UniProt ID: (Mouse) Q62351

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Entrez Gene ID: (Mouse) 22042

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Function(s)

glycoprotein binding double-stranded RNA binding transferrin receptor activity iron ion transmembrane transporter activity protein binding Hsp70 protein binding transferrin transmembrane transporter activity identical protein binding poly(A) RNA binding chaperone binding metalloprotease

Process(es)

cellular iron ion homeostasis endocytosis receptor-mediated endocytosis osteoclast differentiation transferrin transport iron ion transmembrane transport cellular response to drug positive regulation of bone resorption protein transmembrane transport iron ion import

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CD71 (Transferrin Receptor) Monoclonal Antibody (R17217 (RI7 217.1.4)), eFluor™ 450, eBioscience™

CD71 (Transferrin Receptor) Antibody (48-0711-82) in Flow

Product Details

48-0711-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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