Invitrogen

CD122 Monoclonal Antibody (TM-b1 (TM-beta1)), eFluor™ 450, eBioscience™

FIGURE: 1 / 1

CD122 Antibody (48-1222-82) in Flow

Staining of C57Bl/6 splenocytes with Anti-Mouse NK1-1 APC (Product # 17-5941-82) and 0.125 µg of Rat IgG2b K Isotype Control eFluor® 450 (Product # 48-4031-82) (left) or 0.125 µg of Anti-Mouse CD122 eFluor® 450 (right). Total viable cells were used for analysis.

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Product Details

48-1222-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.25 µg/test

View 8 publications 8 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2b, kappa

Recommended Isotype Control

Rat IgG2b kappa Isotype Control (eB149/10H5), eFluor™ 450, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

TM-b1 (TM-beta1)

Conjugate

eFluor™ 450 eFluor™ 450 eFluor™ 450

Excitation/Emission Max

405/445 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2016697

Product Specific Information

Description: The TM-b1 monoclonal antibody reacts with mouse CD122, the 90-110 kDa interleukin-2 receptor beta chain (IL-2R beta). CD122 is expressed by NK cells and some T cells in the periphery, and is upregulated by activation. CD122 associates with CD25 and CD132 (common gamma chain) to form the high affinity IL-2 receptor. High affinity binding of IL-2 to the IL-2 receptor is inhibited by the TM-b1 antibody.

Applications Reported: This TM-b1 (TM-beta1) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This TM-b1 (TM-beta1) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD122 (Interleukin 2 receptor) is involved in T cell-mediated immune responses, primarily expressed in the hematopoietic system and is present in 3 forms. The low affinity form of CD122 is a monomer of the alpha subunit and is not involved in signal transduction. The intermediate affinity form consists of an alpha/beta subunit heterodimer, while the high affinity form consists of an alpha/beta/gamma subunit heterotrimer. Both the intermediate and high affinity forms of the receptor are involved in receptor-mediated endocytosis and transduction of mitogenic signals from CD122. The protein encoded by the CD122 gene represents the beta subunit and is a type I membrane protein. Diseases associate with CD122 protein dysfunction include oligoarticular juvenile idiopathic arthritis and rheumatoid factor negative juvenile idiopathic arthritis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

The transcription factor Bach2 negatively regulates murine natural killer cell maturation and function.

eLife

Li S,Bern MD,Miao B,Fan C,Xing X,Inoue T,Piersma SJ,Wang T,Colonna M,Kurosaki T,Yokoyama WM

48-1222-82 was used in Flow cytometry/Cell sorting to investigate the role of Bach2 in natural killer (NK) cell development, revealing that Bach2 expression peaked in immature CD27+CD11b+ cells, and its deficiency led to increased proportions of terminally differentiated NK cells with enhanced granzyme and cytokine production, as well as improved NK cell-mediated control of tumor metastasis.

Mon Oct 03 00:00:00 EDT 2022

SRC-3 Functions as a Coactivator of T-bet by Regulating the Maturation and Antitumor Activity of Natural Killer Cells.

Cancer immunology research

Hu M,Lu Y,Qi Y,Zhang Z,Wang S,Xu Y,Chen F,Tang Y,Chen S,Chen M,Du C,Shen M,Wang F,Su Y,Deng Y,Wang J

48-1222-82 was used in Flow Cytometry to study the role of SRC-3 as a coactivator of T-bet in natural killer-cell biology and indicate that targeting SRC-3 may be a promising strategy to increase the tumor surveillance function of natural killer cells.

Tue Sep 01 00:00:00 EDT 2020

Control of Viral Infection by Natural Killer Cell Inhibitory Receptors.

Cell reports

Parikh BA,Bern MD,Piersma SJ,Yang L,Beckman DL,Poursine-Laurent J,Plougastel-Douglas B,Yokoyama WM

48-1222 was used in Flow cytometry/Cell sorting to show MHC-I-restricted control of murine cytomegalovirus (MCMV) infection in vivo that is NK cell dependent.

Tue Jul 28 00:00:00 EDT 2020

Stage-Specific Requirement for Eomes in Mature NK Cell Homeostasis and Cytotoxicity.

Cell reports

Wagner JA,Wong P,Schappe T,Berrien-Elliott MM,Cubitt C,Jaeger N,Lee M,Keppel CR,Marin ND,Foltz JA,Marsala L,Neal CC,Sullivan RP,Schneider SE,Keppel MP,Saucier N,Cooper MA,Fehniger TA

48-1222 was used in Flow cytometry/Cell sorting to develop a tamoxifen-inducible, type-1-ILC-specific (Ncr1-targeted) cre mouse and combine this with Eomes-floxed mice.

Tue Jun 02 00:00:00 EDT 2020

The PD-1 Pathway Regulates Development and Function of Memory CD8⁺ T Cells following Respiratory Viral Infection.

Cell reports

Pauken KE,Godec J,Odorizzi PM,Brown KE,Yates KB,Ngiow SF,Burke KP,Maleri S,Grande SM,Francisco LM,Ali MA,Imam S,Freeman GJ,Haining WN,Wherry EJ,Sharpe AH

48-1222-82 was used in Flow Cytometry to demonstrate that PD-1 signals are needed for optimal memory.

Tue Jun 30 00:00:00 EDT 2020

An Id2^RFP-Reporter Mouse Redefines Innate Lymphoid Cell Precursor Potentials.

Immunity

Xu W,Cherrier DE,Chea S,Vosshenrich C,Serafini N,Petit M,Liu P,Golub R,Di Santo JP

48-1222 was used in Flow cytometry/Cell sorting to suggest a revised model for ILC differentiation that redefines the cell-fate potential of helper-ILC-restricted Zbtb16+ ILCPs.

Tue Apr 16 00:00:00 EDT 2019

Repression of p53-target gene Bbc3/PUMA by MYSM1 is essential for the survival of hematopoietic multipotent progenitors and contributes to stem cell maintenance.

Cell death and differentiation

Belle JI,Petrov JC,Langlais D,Robert F,Cencic R,Shen S,Pelletier J,Gros P,Nijnik A

48-1222 was used in Flow cytometry/Cell sorting to elucidate the mechanisms underlying the transcriptional activation of p53 stress responses in Mysm1-deficient haematopoietic stem and progenitor cells.

Sun May 01 00:00:00 EDT 2016

Dendritic cell-MHC class II and Itk regulate functional development of regulatory innate memory CD4+ T cells in bone marrow transplantation.

Journal of immunology (Baltimore, Md. : 1950)

Huang W,Qi Q,Hu J,Huang F,Laufer TM,August A

48-1222 was used in Flow cytometry/Cell sorting to demonstrate that dendritic cells-MHCII and IL-2-inducible T cell kinase regulate the functional development of IMP CD4(+) T cells.

Tue Apr 01 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: CD122; High affinity IL-2 receptor subunit beta; IL-15 receptor beta chain; IL-2 receptor beta chain; IL-2 receptor subunit beta; IL-2/15 receptor-beta; IL-2R subunit beta; IL-2RB; Interleukin-2 receptor subunit beta; p70-75

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Gene Aliases: CD122; IL-15Rbeta; Il-2/15Rbeta; Il-2Rbeta; IL15Rbeta; Il2rb; p70

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UniProt ID: (Mouse) P16297

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Entrez Gene ID: (Mouse) 16185

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Function(s)

cytokine receptor activity interleukin-2 receptor activity interleukin-2 binding transmembrane signal receptor

Process(es)

cytokine-mediated signaling pathway natural killer cell activation interleukin-2-mediated signaling pathway negative regulation of apoptotic process

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CD122 Monoclonal Antibody (TM-b1 (TM-beta1)), eFluor™ 450, eBioscience™

CD122 Antibody (48-1222-82) in Flow

Product Details

48-1222-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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