Invitrogen

4EBP1 Monoclonal Antibody (554R16)

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

3 Published Figures

7 References

View all (67) 4EBP1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

4EBP1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 10

4EBP1 Antibody (AHO1382) in ICC/IF

Immunofluorescence analysis of 4EBP1 was performed using 70% confluent log phase A-431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with 4EBP1 Monoclonal Antibody (Product # AHO1382) at 1:250 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilutio... View More

Product Image Gallery

4EBP1 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. A-431 cells were transfected with 4EBP1 siRNA and decrease in signal was observed in Western Blot using Anti-4EBP1 Monoclonal Antibody (Product # AHO1382). {KD}

Product Details

AHO1382

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

View 5 publications 5 publications

Immunocytochemistry (ICC/IF)

1:250

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Rat

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

554R16

Immunogen

Recombinant human 4E-BP1 protein expressed in E. coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

purified

Storage buffer

PBS, pH 7.2, with 1% BSA

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2536336

Target Information

This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E, which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Pathological mitophagy disrupts mitochondrial homeostasis in Leber's hereditary optic neuropathy.

Cell reports

Danese A,Patergnani S,Maresca A,Peron C,Raimondi A,Caporali L,Marchi S,La Morgia C,Del Dotto V,Zanna C,Iannielli A,Segnali A,Di Meo I,Cavaliere A,Lebiedzinska-Arciszewska M,Wieckowski MR,Martinuzzi A,Moraes-Filho MN,Salomao SR,Berezovsky A,Belfort R,Buser C,Ross-Cisneros FN,Sadun AA,Tacchetti C,Broccoli V,Giorgi C,Tiranti V,Carelli V,Pinton P

AHO1382 was used in Western Blotting to show that a sustained pathological autophagy and compartment-specific mitophagy activity affects Leber's hereditary optic neuropathy patient-derived cells and cybrids, as well as induced pluripotent-stem-cell-derived neurons.

Tue Jul 19 00:00:00 EDT 2022

Inhibition of the autophagic protein ULK1 attenuates axonal degeneration in vitro and in vivo, enhances translation, and modulates splicing.

Cell death and differentiation

Vahsen BF,Ribas VT,Sundermeyer J,Boecker A,Dambeck V,Lenz C,Shomroni O,Caldi Gomes L,Tatenhorst L,Barski E,Roser AE,Michel U,Urlaub H,Salinas G,Bähr M,Koch JC,Lingor P

AHO1382 was used in Western Blotting to study the effect of ULK1 inhibition in different models of lesion-induced axonal degeneration in vitro and in vivo.

Thu Oct 01 00:00:00 EDT 2020

Inhibition of the autophagic protein ULK1 attenuates axonal degeneration in vitro and in vivo, enhances translation, and modulates splicing.

Cell death and differentiation

Vahsen BF,Ribas VT,Sundermeyer J,Boecker A,Dambeck V,Lenz C,Shomroni O,Caldi Gomes L,Tatenhorst L,Barski E,Roser AE,Michel U,Urlaub H,Salinas G,Bähr M,Koch JC,Lingor P

AHO1382 was used in Western Blotting to study the effect of ULK1 inhibition in different models of lesion-induced axonal degeneration in vitro and in vivo.

Thu Oct 01 00:00:00 EDT 2020

Polycystin-1 affects cancer cell behaviour and interacts with mTOR and Jak signalling pathways in cancer cell lines.

Journal of cellular and molecular medicine

Papavassiliou KA,Zoi I,Gargalionis AN,Koutsilieris M

AHO1382 was used in Western Blotting to study the role of polycystin-1 in cancer biology.

Sun Sep 01 00:00:00 EDT 2019

Effect and molecular mechanism of mTOR inhibitor rapamycin on temozolomide-induced autophagic death of U251 glioma cells.

Oncology letters

Li B,Zhou C,Yi L,Xu L,Xu M

AHO1382 was used in Western Blotting to examine the effect of temozolomide (TMZ) combined with rapamycin on the TMZ-induced autophagic death of U251 glioma cells.

Thu Feb 01 00:00:00 EST 2018

Induction of genuine autophagy by cationic lipids in mammalian cells.

Autophagy

Man N,Chen Y,Zheng F,Zhou W,Wen LP

AHO1382 was used in western blot to report that cationic lipids induced autophagy in mammalian cells

Sat May 01 00:00:00 EDT 2010

Butyrate-rich colonic microenvironment is a relevant selection factor for metabolically adapted tumor cells.

The Journal of biological chemistry

Serpa J,Caiado F,Carvalho T,Torre C,Gonçalves LG,Casalou C,Lamosa P,Rodrigues M,Zhu Z,Lam EW,Dias S

Fri Dec 10 00:00:00 EST 2010

Butyrate-rich colonic microenvironment is a relevant selection factor for metabolically adapted tumor cells.

The Journal of biological chemistry

Serpa J,Caiado F,Carvalho T,Torre C,Gonçalves LG,Casalou C,Lamosa P,Rodrigues M,Zhu Z,Lam EW,Dias S

Fri Dec 10 00:00:00 EST 2010

Bioinformatics

Protein Aliases: 4E-BP1; eIF4E-binding protein 1; Eukaryotic translation initiation factor 4E-binding protein 1; MGC431; MGC4316; P/OKCL.6; PHAS-1; PHAS-I; Phosphorylated heat- and acid-stable protein regulated by insulin 1

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Gene Aliases: 4E-BP1; 4EBP1; AA959816; BP-1; EIF4EBP1; PHAS-I

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UniProt ID: (Human) Q13541, (Mouse) Q60876, (Rat) Q62622

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Entrez Gene ID: (Human) 1978, (Mouse) 13685, (Rat) 116636

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Function(s)

protein binding eukaryotic initiation factor 4E binding translation repressor activity protein phosphatase 2A binding translation initiation factor binding translation initiation factor

Process(es)

G1/S transition of mitotic cell cycle IRES-dependent translational initiation response to ischemia insulin receptor signaling pathway lung development negative regulation of protein complex assembly TOR signaling response to ethanol positive regulation of mitotic cell cycle negative regulation of translational initiation cellular response to hypoxia regulation of translation regulation of translational initiation negative regulation of translation

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4EBP1 Monoclonal Antibody (554R16)

4EBP1 Antibody (AHO1382) in ICC/IF

Product Details

AHO1382

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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