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Invitrogen
5-Carboxylcytosine Recombinant Rabbit Monoclonal Antibody (RM462)
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5-Carboxylcytosine Antibody (MA5-44548) in ELISA
ELISA analysis of DNA using 5-Carboxylcytosine Recombinant Monoclonal antibody (Product # MA5-44548) at a dilution of 1 µg/mL. The plate was coated with different concentrations of linear ssDNA (426 bp) containing either normal cytosines (unmodified), 5-methylcytosines (5-mC), 5-hydroxymethylcytosines (5-hmC), 5-carboxylcytosines (5-caC), or 5-formylcytosines (5-fC).
Product Details
MA5-44548
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chemical
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
RM462
Immunogen
KLH-conjugated 5-Carboxylcytosine.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS with 1% BSA, 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2926678
Product Specific Information
This antibody reacts to human NeuN (Fox3, RBFOX3). The clone may also react to mouse or rat NeuN, as predicted by immunogen homology.
Target Information
5-carboxylcytosine is the final oxidized derivative of 5-methylcytosine, a type of DNA methylation. 5-methylcytosine is oxidized to 5-hydroxymethylcytosine which is then oxidized to 5-formylcytosine and later to 5-carboxylcytosine. These oxidations are catalyzed by the Ten-Eleven Translocation (TET) enzyme family. 5-carboxylcytosine has been shown to cause reduced fidelity of RNA Polymerase II. Decreased levels of 5-hydroxymethylcytosine has been found in some cancers.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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