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Active Motif
5-Formylcytosine (5-fC) Polyclonal Antibody
FIGURE: 1 / 1
5-Formylcytosine (5-fC) Antibody (61227) in DB
Dot blot of 5-Formylcytosine pAb. Dot blot analysis was used to confirm the specificity of 5-Formylcytosine antibody for 5-Formylcytosine (5-formylcytidine, 5-fC). Single-stranded 38 nt DNA oligonucleotides (amount of oligo in nanograms listed on the left side of the blot) corresponding to the immunogen and related sequences were spotted onto nitrocellulose and probed with the antibody at 0.2 µg/ml. Lane 1: oligo containing 5-carboxylcytidine. Lane 2: oligo containing 5-formylcytidine. Lane 3: oligo containing 5-hydroxymethylcytidine. Lane... View More
Product Details
61227
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chemical
Published species
Chemical,
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
This 5-Formylcytosine antibody was raised against 5-formylcytidine conjugated to kLH and recognizes 5-formylcytosine.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.5, with 30% glycerol
Contains
0.035% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2793558
Target Information
5-formylcytosine is an oxidized derivative of 5-methylcytosine, a type of DNA methylation. 5-formylcytosine is a nucleobase analogue with a cytosine that possesses a hydrogen at position 5 that has been replaced by a formyl group. 5-methylcytosine is oxidized to 5-hydroxymethylcytosine which is then oxidized to 5-formylcytosine and later to 5-carboxylcytosine. These oxidations are catalyzed by the Ten-Eleven Translocation (TET) enzyme family. 5-formylcytosine has been shown to determine nucleosome organization and plays a role in regulating transcription.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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