Active Motif

5-Hydroxymethylcytosine (5-hmC) Polyclonal Antibody

5-Hydroxymethylcytosine (5-hmC) Antibody in Methylated DNA Immunoprecipitation (MeDIP)

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5-Hydroxymethylcytosine (5-hmC) Antibody (39769) in MeDIP

5-Hydroxymethylcytosine (5-hmC, 5-hydroxymethylcytidine) antibody tested by Methyl DNA immunoprecipitation. DNA (25 pg) derived from the promoter of the APC gene was spiked into 500 ng of human genomic DNA and subjected to the MeDIP procedure using 1 µL of 5 Hydroxymethylcytidine antibody (5hmC, maroon bars) or 1 µL of control rabbit IgG (IgG, blue bars). Real time quantitative PCR was performed on the immu... View More

5-Hydroxymethylcytosine (5-hmC) Antibody in Dot Blot (DB)

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Product Details

39769

Applications

Tested Dilution

Publications

Western Blot (WB)

View 2 publications 2 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 5 publications 5 publications

Flow Cytometry (Flow)

View 1 publication 1 publication

ChIP-sequencing (ChIP-Seq)

View 2 publications 2 publications

Dot blot (DB)

1:10,000

View 8 publications 8 publications

Immunomicroscopy (IM)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 18 publications 18 publications

Immunostaining (IS)

View 1 publication 1 publication

Methylated DNA Immunoprecipitation (MeDIP)

0.1-5 µL

View 4 publications 4 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Insect, Mouse, Non-human primate, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

This 5-Hydroxymethylcytosine antibody was raised against 5-hydroxymethylcytidine conjugated to kLH and recognizes 5-hydroxymethylcytosine.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

whole serum with 30% glycerol

Contains

0.035% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_10013602

Target Information

5-hydroxymethylcytosine (5-hmC) has been recently discovered in mammalian DNA. 5-hmC results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of oxygenases. Initially, the 5-hmC base had been identified in Purkinje neurons, in granule cells and embryonic stem cells where it is present at high levels (up to 0,6% of total nucleotides in Purkinje cells). A recent report indicates that 5-hmC is also abundant in brain tissue, especially in areas that are associated with higher cognitive functions.Early evidence suggests that 5-hmC may represent an intermediate in a new pathway which demethylates DNA, converting 5-mC to cytosine. Although its precise role has still to be shown, recent results indicate, however, that 5-hmC may play important roles distinct from 5-mC. This may open up entirely new perspectives in epigenetic studies.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Small-Molecule Targeting of Oncogenic FTO Demethylase in Acute Myeloid Leukemia.

Cancer cell

Huang Y,Su R,Sheng Y,Dong L,Dong Z,Xu H,Ni T,Zhang ZS,Zhang T,Li C,Han L,Zhu Z,Lian F,Wei J,Deng Q,Wang Y,Wunderlich M,Gao Z,Pan G,Zhong D,Zhou H,Zhang N,Gan J,Jiang H,Mulloy JC,Qian Z,Chen J,Yang CG

Research topic: acute myeloid leukemia (AML)

Mon Apr 15 00:00:00 EDT 2019

TET1 reprograms the epithelial ovarian cancer epigenome and reveals casein kinase 2α as a therapeutic target.

The Journal of pathology

Chen LY,Huang RL,Chan MW,Yan PS,Huang TS,Wu RC,Suryo Rahmanto Y,Su PH,Weng YC,Chou JL,Chao TK,Wang YC,Shih IM,Lai HC

Research topic: Ovarian Cancer

Mon Jul 01 00:00:00 EDT 2019

TET1 promotes 5hmC-dependent stemness, and inhibits a 5hmC-independent epithelial-mesenchymal transition, in cervical precancerous lesions.

Cancer letters

Su PH,Hsu YW,Huang RL,Chen LY,Chao TK,Liao CC,Chen CW,Wu TI,Mao SP,Balch C,Lai HC

Research topic: Cervical Cancer, DNA Methylation

Tue May 28 00:00:00 EDT 2019

Reversal of TET-mediated 5-hmC loss in hypoxic fibroblasts by ascorbic acid.

Laboratory investigation; a journal of technical methods and pathology

Liu Y,Xu S,Zu T,Li F,Sang S,Liu C,An Y,Mi B,Orgill DP,Murphy GF,Lian CG

Research topic: DNA Methylation, Hypoxia

Mon Jul 01 00:00:00 EDT 2019

Global DNA Methylation and Histone Posttranslational Modifications in Human and Nonhuman Primate Brain in Association with Prenatal Alcohol Exposure.

Alcoholism, clinical and experimental research

Jarmasz JS,Stirton H,Basalah D,Davie JR,Clarren SK,Astley SJ,Del Bigio MR

Research topic: Neurobiology, Alcoholism

Sat Jun 01 00:00:00 EDT 2019

Global DNA Methylation and Histone Posttranslational Modifications in Human and Nonhuman Primate Brain in Association with Prenatal Alcohol Exposure.

Alcoholism, clinical and experimental research

Jarmasz JS,Stirton H,Basalah D,Davie JR,Clarren SK,Astley SJ,Del Bigio MR

Research topic: Neurobiology, Alcoholism

Sat Jun 01 00:00:00 EDT 2019

5hmC Level Predicts Biochemical Failure Following Radical Prostatectomy in Prostate Cancer Patients with ERG Negative Tumors.

International journal of molecular sciences

Kristensen G,Strand SH,Røder MA,Berg KD,Toft BG,Høyer S,Borre M,Sørensen KD,Brasso K

Research topic: Prostate Cancer

Wed Feb 27 00:00:00 EST 2019

Tet inactivation disrupts YY1 binding and long-range chromatin interactions during embryonic heart development.

Nature communications

Fang S,Li J,Xiao Y,Lee M,Guo L,Han W,Li T,Hill MC,Hong T,Mo W,Xu R,Zhang P,Wang F,Chang J,Zhou Y,Sun D,Martin JF,Huang Y

Research topic: Cardiology, Gene Expression

Fri Sep 20 00:00:00 EDT 2019

Rinf Regulates Pluripotency Network Genes and Tet Enzymes in Embryonic Stem Cells.

Cell reports

Ravichandran M,Lei R,Tang Q,Zhao Y,Lee J,Ma L,Chrysanthou S,Lorton BM,Cvekl A,Shechter D,Zheng D,Dawlaty MM

Research topic: Transcription, Stem Cells

Tue Aug 20 00:00:00 EDT 2019

Mutant IDH1 confers resistance to energy stress in normal biliary cells through PFKP-induced aerobic glycolysis and AMPK activation.

Scientific reports

Fujiwara H,Tateishi K,Misumi K,Hayashi A,Igarashi K,Kato H,Nakatsuka T,Suzuki N,Yamamoto K,Kudo Y,Hayakawa Y,Nakagawa H,Tanaka Y,Ijichi H,Kogure H,Nakai Y,Isayama H,Hasegawa K,Fukayama M,Soga T,Koike K

Research topic: Metabollism, Cancer

Wed Dec 11 00:00:00 EST 2019

CHD3 and CHD4 recruitment and chromatin remodeling activity at DNA breaks is promoted by early poly(ADP-ribose)-dependent chromatin relaxation.

Nucleic acids research

Smith R,Sellou H,Chapuis C,Huet S,Timinszky G

Research topic: fatty liver disease (NAFLD)

Fri Jul 06 00:00:00 EDT 2018

Decoding the chromatin proteome of a single genomic locus by DNA sequencing.

PLoS biology

Korthout T,Poramba-Liyanage DW,van Kruijsbergen I,Verzijlbergen KF,van Gemert FPA,van Welsem T,van Leeuwen F

Research topic: Cancer - Bladder

Sun Jul 01 00:00:00 EDT 2018

TET1 exerts its anti-tumor functions via demethylating DACT2 and SFRP2 to antagonize Wnt/β-catenin signaling pathway in nasopharyngeal carcinoma cells.

Clinical epigenetics

Fan J,Zhang Y,Mu J,He X,Shao B,Zhou D,Peng W,Tang J,Jiang Y,Ren G,Xiang T

Research topic: Nasopharyngeal Carcinoma

Fri Aug 03 00:00:00 EDT 2018

Modelling non-alcoholic fatty liver disease in human hepatocyte-like cells.

Philosophical transactions of the Royal Society of London. Series B, Biological sciences

Lyall MJ,Cartier J,Thomson JP,Cameron K,Meseguer-Ripolles J,O'Duibhir E,Szkolnicka D,Villarin BL,Wang Y,Blanco GR,Dunn WB,Meehan RR,Hay DC,Drake AJ

Research topic: Non-alcoholic fatty liver disease (NAFLD)

Thu Jul 05 00:00:00 EDT 2018

Identification of SMCHD1 domains for nuclear localization, homo-dimerization, and protein cleavage.

Skeletal muscle

Hiramuki Y,Tapscott SJ

Research topic: Embryogenesis, DNA Methylation

Thu Aug 02 00:00:00 EDT 2018

Distinctive epigenomes characterize glioma stem cells and their response to differentiation cues.

Genome biology

Zhou D,Alver BM,Li S,Hlady RA,Thompson JJ,Schroeder MA,Lee JH,Qiu J,Schwartz PH,Sarkaria JN,Robertson KD

Research topic: Brain & Nerves

Tue Mar 27 00:00:00 EDT 2018

Defining baseline epigenetic landscapes in the rat liver.

Epigenomics

Thomson JP,Ottaviano R,Buesen R,Moggs JG,Schwarz M,Meehan RR

Research topic: Liver

Fri Dec 01 00:00:00 EST 2017

Reprogramming towards totipotency is greatly facilitated by synergistic effects of small molecules.

Biology open

Miyamoto K,Tajima Y,Yoshida K,Oikawa M,Azuma R,Allen GE,Tsujikawa T,Tsukaguchi T,Bradshaw CR,Jullien J,Yamagata K,Matsumoto K,Anzai M,Imai H,Gurdon JB,Yamada M

Research topic: Embryogenesis, Animal cloning

Sat Apr 15 00:00:00 EDT 2017

Bi-directional and shared epigenomic signatures following proton and ⁵⁶Fe irradiation.

Scientific reports

Impey S,Jopson T,Pelz C,Tafessu A,Fareh F,Zuloaga D,Marzulla T,Riparip LK,Stewart B,Rosi S,Turker MS,Raber J

Research topic: Brain & Nerves

Thu Aug 31 00:00:00 EDT 2017

Ten-Eleven Translocation-2 (Tet2) Is Involved in Myogenic Differentiation of Skeletal Myoblast Cells in Vitro.

Scientific reports

Zhong X,Wang QQ,Li JW,Zhang YM,An XR,Hou J

Research topic: Muscle differentiation

Wed Mar 08 00:00:00 EST 2017

IDH1 or -2 mutations do not predict outcome and do not cause loss of 5-hydroxymethylcytosine or altered histone modifications in central chondrosarcomas.

Clinical sarcoma research

Cleven AHG,Suijker J,Agrogiannis G,Briaire-de Bruijn IH,Frizzell N,Hoekstra AS,Wijers-Koster PM,Cleton-Jansen AM,Bovée JVMG

Research topic: Bone - Cancer

Thu Mar 31 00:00:00 EDT 2022

Hepatic stellate cell transdifferentiation involves genome-wide remodeling of the DNA methylation landscape.

Journal of hepatology

Page A,Paoli P,Moran Salvador E,White S,French J,Mann J

Research topic: Liver Fibrosis

Tue Mar 01 00:00:00 EST 2016

Hepatic stellate cell transdifferentiation involves genome-wide remodeling of the DNA methylation landscape.

Journal of hepatology

Page A,Paoli P,Moran Salvador E,White S,French J,Mann J

Research topic: Liver Fibrosis

Tue Mar 01 00:00:00 EST 2016

Hepatic stellate cell transdifferentiation involves genome-wide remodeling of the DNA methylation landscape.

Journal of hepatology

Page A,Paoli P,Moran Salvador E,White S,French J,Mann J

Research topic: Liver Fibrosis

Tue Mar 01 00:00:00 EST 2016

TET-catalyzed oxidation of intragenic 5-methylcytosine regulates CTCF-dependent alternative splicing.

The EMBO journal

Marina RJ,Sturgill D,Bailly MA,Thenoz M,Varma G,Prigge MF,Nanan KK,Shukla S,Haque N,Oberdoerffer S

Research topic: RNA splicing

Mon Feb 01 00:00:00 EST 2016

Loss of Tet1-Associated 5-Hydroxymethylcytosine Is Concomitant with Aberrant Promoter Hypermethylation in Liver Cancer.

Cancer research

Thomson JP,Ottaviano R,Unterberger EB,Lempiäinen H,Muller A,Terranova R,Illingworth RS,Webb S,Kerr AR,Lyall MJ,Drake AJ,Wolf CR,Moggs JG,Schwarz M,Meehan RR

Research topic: Liver

Sun May 15 00:00:00 EDT 2016

DNA methylation changes induced by long and short photoperiods in Nasonia.

Genome research

Pegoraro M,Bafna A,Davies NJ,Shuker DM,Tauber E

Research topic: DNA methylation in insect photoperiodic timing

Mon Feb 01 00:00:00 EST 2016

Reciprocal changes in DNA methylation and hydroxymethylation and a broad repressive epigenetic switch characterize FMR1 transcriptional silencing in fragile X syndrome.

Clinical epigenetics

Brasa S,Mueller A,Jacquemont S,Hahne F,Rozenberg I,Peters T,He Y,McCormack C,Gasparini F,Chibout SD,Grenet O,Moggs J,Gomez-Mancilla B,Terranova R

Research topic: Fragile X syndrome (FXS)

Fri Apr 22 00:00:00 EDT 2016

DNA cytosine hydroxymethylation levels are distinct among non-overlapping classes of peripheral blood leukocytes.

Journal of immunological methods

Hohos NM,Lee K,Ji L,Yu M,Kandasamy MM,Phillips BG,Baile CA,He C,Schmitz RJ,Meagher RB

Research topic: Immunology & Hematology

Thu Sep 01 00:00:00 EDT 2016

DNA cytosine hydroxymethylation levels are distinct among non-overlapping classes of peripheral blood leukocytes.

Journal of immunological methods

Hohos NM,Lee K,Ji L,Yu M,Kandasamy MM,Phillips BG,Baile CA,He C,Schmitz RJ,Meagher RB

Research topic: Immunology & Hematology

Thu Sep 01 00:00:00 EDT 2016

TET1 Depletion Induces Aberrant CpG Methylation in Colorectal Cancer Cells.

PloS one

Kai M,Niinuma T,Kitajima H,Yamamoto E,Harada T,Aoki H,Maruyama R,Toyota M,Sasaki Y,Sugai T,Tokino T,Nakase H,Suzuki H

Research topic: Colorectal

Wed Jul 05 00:00:00 EDT 2017

Characterization of preneoplastic and neoplastic rat mesothelial cell lines: the involvement of TETs, DNMTs, and 5-hydroxymethylcytosine.

Oncotarget

Roulois D,Deshayes S,Guilly MN,Nader JS,Liddell C,Robard M,Hulin P,Ouacher A,Le Martelot V,Fonteneau JF,Grégoire M,Blanquart C,Pouliquen DL

Research topic: Cancer - Malignant Mesothelioma

Tue Jun 07 00:00:00 EDT 2016

Characterization of preneoplastic and neoplastic rat mesothelial cell lines: the involvement of TETs, DNMTs, and 5-hydroxymethylcytosine.

Oncotarget

Roulois D,Deshayes S,Guilly MN,Nader JS,Liddell C,Robard M,Hulin P,Ouacher A,Le Martelot V,Fonteneau JF,Grégoire M,Blanquart C,Pouliquen DL

Research topic: Cancer - Malignant Mesothelioma

Tue Jun 07 00:00:00 EDT 2016

Anabolic and Antiresorptive Modulation of Bone Homeostasis by the Epigenetic Modulator Sulforaphane, a Naturally Occurring Isothiocyanate.

The Journal of biological chemistry

Thaler R,Maurizi A,Roschger P,Sturmlechner I,Khani F,Spitzer S,Rumpler M,Zwerina J,Karlic H,Dudakovic A,Klaushofer K,Teti A,Rucci N,Varga F,van Wijnen AJ

Research topic: Osteoporosis

Fri Mar 25 00:00:00 EDT 2016

Loss of 5-Hydroxymethylcytosine Is an Independent Unfavorable Prognostic Factor for Esophageal Squamous Cell Carcinoma.

PloS one

Shi X,Yu Y,Luo M,Zhang Z,Shi S,Feng X,Chen Z,He J

Research topic: Cancer - Esophageal Squamous Cell Carcinoma

Mon Aug 22 00:00:00 EDT 2016

Loss of 5-Hydroxymethylcytosine Is an Independent Unfavorable Prognostic Factor for Esophageal Squamous Cell Carcinoma.

PloS one

Shi X,Yu Y,Luo M,Zhang Z,Shi S,Feng X,Chen Z,He J

Research topic: Cancer - Esophageal Squamous Cell Carcinoma

Mon Aug 22 00:00:00 EDT 2016

Genome-wide alteration of 5-hydroxymenthylcytosine in a mouse model of Alzheimer's disease.

BMC genomics

Shu L,Sun W,Li L,Xu Z,Lin L,Xie P,Shen H,Huang L,Xu Q,Jin P,Li X

Research topic: Brain & Nerves - Alzheimers Disease

Fri May 20 00:00:00 EDT 2016

SOX17 is a critical specifier of human primordial germ cell fate.

Cell

Irie N,Weinberger L,Tang WW,Kobayashi T,Viukov S,Manor YS,Dietmann S,Hanna JH,Surani MA

Thu Jan 15 00:00:00 EST 2015

High levels of 5-hydroxymethylcytosine (5hmC) is an adverse predictor of biochemical recurrence after prostatectomy in ERG-negative prostate cancer.

Clinical epigenetics

Strand SH,Hoyer S,Lynnerup AS,Haldrup C,Storebjerg TM,Borre M,Orntoft TF,Sorensen KD

Research topic: Prostate Cancer

Mon Oct 19 00:00:00 EDT 2015

D-2-hydroxyglutarate is essential for maintaining oncogenic property of mutant IDH-containing cancer cells but dispensable for cell growth.

Oncotarget

Ma S,Jiang B,Deng W,Gu ZK,Wu FZ,Li T,Xia Y,Yang H,Ye D,Xiong Y,Guan KL

Research topic: Cancer

Mon Apr 20 00:00:00 EDT 2015

TET1-mediated different transcriptional regulation in prostate cancer.

International journal of clinical and experimental medicine

Feng J,Wang Q,Li G,Zeng X,Kuang S,Li X,Yue Y

Research topic: Prostate Cancer

Wed Mar 18 00:00:00 EDT 2015

Resetting transcription factor control circuitry toward ground-state pluripotency in human.

Cell

Takashima Y,Guo G,Loos R,Nichols J,Ficz G,Krueger F,Oxley D,Santos F,Clarke J,Mansfield W,Reik W,Bertone P,Smith A

Research topic: Stem Cells

Thu Sep 11 00:00:00 EDT 2014

TET1 is a maintenance DNA demethylase that prevents methylation spreading in differentiated cells.

Nucleic acids research

Jin C,Lu Y,Jelinek J,Liang S,Estecio MR,Barton MC,Issa JP

Research topic: DNA Methylation

Sun Jun 01 00:00:00 EDT 2014

Polycomb binding precedes early-life stress responsive DNA methylation at the Avp enhancer.

PloS one

Murgatroyd C,Spengler D

Research topic: Neurobiology, Developmental Biology

Tue Jan 13 00:00:00 EST 2015

Cancer-associated IDH2 mutants drive an acute myeloid leukemia that is susceptible to Brd4 inhibition.

Genes & development

Chen C,Liu Y,Lu C,Cross JR,Morris JP,Shroff AS,Ward PS,Bradner JE,Thompson C,Lowe SW

Research topic: Leukemia (AML)

Sun Sep 15 00:00:00 EDT 2013

Cancer-associated IDH2 mutants drive an acute myeloid leukemia that is susceptible to Brd4 inhibition.

Genes & development

Chen C,Liu Y,Lu C,Cross JR,Morris JP,Shroff AS,Ward PS,Bradner JE,Thompson C,Lowe SW

Research topic: Leukemia (AML)

Sun Sep 15 00:00:00 EDT 2013

5'-Methylcytosine and 5'-hydroxymethylcytosine each provide epigenetic information to the mouse zygote.

PloS one

Li Y,O'Neill C

Research topic: Embryogenesis

Mon Dec 30 00:00:00 EST 2013

Analysis of TET expression/activity and 5mC oxidation during normal and malignant germ cell development.

PloS one

Nettersheim D,Heukamp LC,Fronhoffs F,Grewe MJ,Haas N,Waha A,Honecker F,Waha A,Kristiansen G,Schorle H

Research topic: Germ Cell Development

Tue Sep 30 00:00:00 EDT 2014

Analysis of TET expression/activity and 5mC oxidation during normal and malignant germ cell development.

PloS one

Nettersheim D,Heukamp LC,Fronhoffs F,Grewe MJ,Haas N,Waha A,Honecker F,Waha A,Kristiansen G,Schorle H

Research topic: Germ Cell Development

Tue Sep 30 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: 5-hmc; 5-HydroxymethylCytidine; 5hmc; C5H7N3O2

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5-Hydroxymethylcytosine (5-hmC) Polyclonal Antibody

5-Hydroxymethylcytosine (5-hmC) Antibody (39769) in MeDIP

Product Details

39769

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ChIP-sequencing (ChIP-Seq)

Dot blot (DB)

Immunomicroscopy (IM)

Miscellaneous PubMed (Misc)

Immunostaining (IS)

Methylated DNA Immunoprecipitation (MeDIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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