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Active Motif
5-Hydroxymethylcytosine (5-hmC) Polyclonal Antibody
FIGURE: 1 / 3
5-Hydroxymethylcytosine (5-hmC) Antibody (39791) in IHC
5-Hydroxymethylcytosine (5-hmc) antibody (pAb) tested by Immunohistochemistry Punctate nuclear staining pattern is detected in Formalin-fixed, paraffin-embedded tissue sections from human substantia nigra (midbrain). Top Panel: 5-mC antibody at 1:1000 dilution. Bottom Panel: No primary antibody (2nd step antibody alone).
Product Details
39791
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human,
Mouse,
Zebrafish
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
This 5-Hydroxymethylcytidine antibody was raised against 5-hydroxymethylcytidine conjugated to kLH and recognizes 5-hydroxymethylcytosine.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.5, with 30% glycerol
Contains
0.035% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2630381
Target Information
5-hydroxymethylcytosine (5-hmC) has been recently discovered in mammalian DNA. 5-hmC results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of oxygenases. Initially, the 5-hmC base had been identified in Purkinje neurons, in granule cells and embryonic stem cells where it is present at high levels (up to 0,6% of total nucleotides in Purkinje cells). A recent report indicates that 5-hmC is also abundant in brain tissue, especially in areas that are associated with higher cognitive functions.Early evidence suggests that 5-hmC may represent an intermediate in a new pathway which demethylates DNA, converting 5-mC to cytosine. Although its precise role has still to be shown, recent results indicate, however, that 5-hmC may play important roles distinct from 5-mC. This may open up entirely new perspectives in epigenetic studies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 5-hmc; 5-HydroxymethylCytidine; 5hmc; C5H7N3O2
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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