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Active Motif
5-Hydroxymethylcytosine (5-hmC) Monoclonal Antibody (59.1)
FIGURE: 1 / 4
5-Hydroxymethylcytosine (5-hmC) Antibody (39999) in MeDIP
hMeDIP-chip performed on human brain DNA using 5-Hydroxymethylcytosine (5-hmC) antibody. Human brain DNA (2 µg) was immunoprecipitated with 10 µg of 5-Hydroxymethylcytosine antibody. Following hMeDIP, the DNA was amplified, labeled and hybridized to an Affymetrix Human Tiling 2.0R Array. Shown is a region from chromosome 6q containing the ARID1B, ZDHHC14 and SNX9 genes. The results show that 5-hydroxymethyl... View More
Product Details
39999
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human,
Mouse
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
59.1
Immunogen
This 5-Hydroxymethylcytosine antibody was raised against 5-hydroxymethylcytidine conjugated to kLH and recognizes 5-hydroxymethylcytosine.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 8, with 30% glycerol
Contains
0.035% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2566808
Target Information
5-hydroxymethylcytosine (5-hmC) has been recently discovered in mammalian DNA. 5-hmC results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of oxygenases. Initially, the 5-hmC base had been identified in Purkinje neurons, in granule cells and embryonic stem cells where it is present at high levels (up to 0,6% of total nucleotides in Purkinje cells). A recent report indicates that 5-hmC is also abundant in brain tissue, especially in areas that are associated with higher cognitive functions.Early evidence suggests that 5-hmC may represent an intermediate in a new pathway which demethylates DNA, converting 5-mC to cytosine. Although its precise role has still to be shown, recent results indicate, however, that 5-hmC may play important roles distinct from 5-mC. This may open up entirely new perspectives in epigenetic studies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 5-hmc; 5-HydroxymethylCytidine; 5hmc; C5H7N3O2
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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