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Invitrogen
5-Hydroxymethylcytosine (5-hmC) Monoclonal Antibody (GT513)
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5-Hydroxymethylcytosine (5-hmC) Antibody (MA5-42319) in MeDIP
Methylated DNA Immunoprecipitation of 5-Hydroxymethylcytosine (5-hmC) in 500 ng of human genomic DNA mixed with methylated cytosine standard kit. Sample was incubated with monoclonal 5-Hydroxymethylcytosine (5-hmC) antibody (Product # MA5-42319) at a dilution of 1:500.
Product Details
MA5-42319
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chemical
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
GT513
Immunogen
The immunogen used to generate this antibody corresponds to 5-Hydroxymethylcytosine / 5-hmC
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2911461
Product Specific Information
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Target Information
5-hydroxymethylcytosine (5-hmC) has been recently discovered in mammalian DNA. 5-hmC results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of oxygenases. Initially, the 5-hmC base had been identified in Purkinje neurons, in granule cells and embryonic stem cells where it is present at high levels (up to 0,6% of total nucleotides in Purkinje cells). A recent report indicates that 5-hmC is also abundant in brain tissue, especially in areas that are associated with higher cognitive functions.Early evidence suggests that 5-hmC may represent an intermediate in a new pathway which demethylates DNA, converting 5-mC to cytosine. Although its precise role has still to be shown, recent results indicate, however, that 5-hmC may play important roles distinct from 5-mC. This may open up entirely new perspectives in epigenetic studies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 5-hmc; 5-HydroxymethylCytidine; 5hmc; C5H7N3O2
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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