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5 Lipoxygenase Polyclonal Antibody

View all (45) ALOX5 antibodies

Questions & Answers

Questions & Answers

Antibody Testing Data (4)

5 Lipoxygenase Antibody in Immunocytochemistry (ICC/IF)

5 Lipoxygenase Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 4

5 Lipoxygenase Antibody (10021-1-IG) in ICC/IF

Immunofluorescent analysis of Hela cells, using ALOX5 antibody 10021-1-IG at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

5 Lipoxygenase Antibody in Immunocytochemistry (ICC/IF)

5 Lipoxygenase Antibody in Western Blot (WB)

5 Lipoxygenase Antibody in Western Blot (WB)

5 Lipoxygenase Antibody in Western Blot (WB)

Product Image Gallery

5 Lipoxygenase Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of Hela cells, using ALOX5 antibody 10021-1-IG at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

5 Lipoxygenase Antibody in Western Blot (WB) — DU 145 cells were subjected to SDS PAGE followed by western blot with 10021-1-Ig (5 Lipoxygenase antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

5 Lipoxygenase Antibody in Western Blot (WB) — HL-60 cells were subjected to SDS PAGE followed by western blot with 10021-1-Ig (5 Lipoxygenase antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

5 Lipoxygenase Antibody in Western Blot (WB) — Various lysates were subjected to SDS PAGE followed by western blot with 10021-1-IG (5 Lipoxygenase antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Product Details

10021-1-IG

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 8 publications 8 publications

Immunohistochemistry (IHC)

-

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

1:10-1:100

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.53 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Target Information

This gene encodes a protein which, with Arachidonate 5-lipoxygenase-activating protein, is required for leukotriene synthesis. Leukotrienes are arachidonic acid metabolites which have been implicated in various types of inflammatory responses, including asthma, arthritis and psoriasis. This protein localizes to the plasma membrane. Inhibitors of its function impede translocation of 5-lipoxygenase from the cytoplasm to the cell membrane and inhibit 5-lipoxygenase activation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The ALOX5 inhibitor Zileuton regulates tumor-associated macrophage M2 polarization by JAK/STAT and inhibits pancreatic cancer invasion and metastasis.

International immunopharmacology

Hu WM,Liu SQ,Zhu KF,Li W,Yang ZJ,Yang Q,Zhu ZC,Chang J

Tue Aug 01 00:00:00 EDT 2023

The ALOX5 inhibitor Zileuton regulates tumor-associated macrophage M2 polarization by JAK/STAT and inhibits pancreatic cancer invasion and metastasis.

International immunopharmacology

Hu WM,Liu SQ,Zhu KF,Li W,Yang ZJ,Yang Q,Zhu ZC,Chang J

Tue Aug 01 00:00:00 EDT 2023

The ALOX5 inhibitor Zileuton regulates tumor-associated macrophage M2 polarization by JAK/STAT and inhibits pancreatic cancer invasion and metastasis.

International immunopharmacology

Hu WM,Liu SQ,Zhu KF,Li W,Yang ZJ,Yang Q,Zhu ZC,Chang J

Tue Aug 01 00:00:00 EDT 2023

The ALOX5 inhibitor Zileuton regulates tumor-associated macrophage M2 polarization by JAK/STAT and inhibits pancreatic cancer invasion and metastasis.

International immunopharmacology

Hu WM,Liu SQ,Zhu KF,Li W,Yang ZJ,Yang Q,Zhu ZC,Chang J

Tue Aug 01 00:00:00 EDT 2023

The ALOX5 inhibitor Zileuton regulates tumor-associated macrophage M2 polarization by JAK/STAT and inhibits pancreatic cancer invasion and metastasis.

International immunopharmacology

Hu WM,Liu SQ,Zhu KF,Li W,Yang ZJ,Yang Q,Zhu ZC,Chang J

Tue Aug 01 00:00:00 EDT 2023

The ALOX5 inhibitor Zileuton regulates tumor-associated macrophage M2 polarization by JAK/STAT and inhibits pancreatic cancer invasion and metastasis.

International immunopharmacology

Hu WM,Liu SQ,Zhu KF,Li W,Yang ZJ,Yang Q,Zhu ZC,Chang J

Tue Aug 01 00:00:00 EDT 2023

Fenofibrate suppresses corneal neovascularization by regulating lipid metabolism through PPARα signaling pathway.

Frontiers in pharmacology

Zhou T,Yan K,Zhang Y,Zhu L,Liao Y,Zheng X,Chen Y,Li X,Liu Z,Zhang Z

Tue Jan 03 00:00:00 EST 2023

Fenofibrate suppresses corneal neovascularization by regulating lipid metabolism through PPARα signaling pathway.

Frontiers in pharmacology

Zhou T,Yan K,Zhang Y,Zhu L,Liao Y,Zheng X,Chen Y,Li X,Liu Z,Zhang Z

Tue Jan 03 00:00:00 EST 2023

Targeting Circulating lncRNA ENST00000538705.1 Relieves Acute Coronary Syndrome via Modulating ALOX15.

Disease markers

Chen H,Huang S,Guan F,Han S,Ye F,Li X,You L

Thu May 19 00:00:00 EDT 2022

Lipoxin A₄ and its analog attenuate high fat diet-induced atherosclerosis via Keap1/Nrf2 pathway.

Experimental cell research

Xu F,Zhang J,Zhou X,Hao H

Tue Mar 01 00:00:00 EST 2022

Respiratory Syncytial Virus Nonstructural Protein 1 Promotes 5-Lipoxygenase via miR-19a-3p.

Journal of immunology research

Zheng M,Fan P,Yang P,Zheng J,Zhao D

Thu Jun 02 00:00:00 EDT 2022

PPARα agonist WY-14,643 induces adipose atrophy and fails to blunt chronic ethanol-induced hepatic fat accumulation in mice lacking adipose FGFR1.

Biochemical pharmacology

Xu Y,Denning KL,Lu Y

Fri Oct 01 00:00:00 EDT 2021

Comprehensive Analysis of the Tumor Microenvironment and Ferroptosis-Related Genes Predict Prognosis with Ovarian Cancer.

Frontiers in genetics

Li XX,Xiong L,Wen Y,Zhang ZJ

Tue Dec 07 00:00:00 EST 2021

MK591, a second generation leukotriene biosynthesis inhibitor, prevents invasion and induces apoptosis in the bone-invading C4-2B human prostate cancer cells: implications for the treatment of castration-resistant, bone-metastatic prostate cancer.

PloS one

Sarveswaran S,Ghosh R,Morisetty S,Ghosh J

Fri Jan 15 00:00:00 EST 2016

MK591, a second generation leukotriene biosynthesis inhibitor, prevents invasion and induces apoptosis in the bone-invading C4-2B human prostate cancer cells: implications for the treatment of castration-resistant, bone-metastatic prostate cancer.

PloS one

Sarveswaran S,Ghosh R,Morisetty S,Ghosh J

Fri Jan 15 00:00:00 EST 2016

Inhibition of 5-lipoxygenase selectively triggers disruption of c-Myc signaling in prostate cancer cells.

The Journal of biological chemistry

Sarveswaran S,Chakraborty D,Chitale D,Sears R,Ghosh J

Fri Feb 20 00:00:00 EST 2015

Inhibition of 5-lipoxygenase selectively triggers disruption of c-Myc signaling in prostate cancer cells.

The Journal of biological chemistry

Sarveswaran S,Chakraborty D,Chitale D,Sears R,Ghosh J

Fri Feb 20 00:00:00 EST 2015

Bioinformatics

Protein Aliases: 5-lipoxygenase; 5-LO; Arachidonate 5-lipoxygenase; arachidonic 5-lipoxygenase alpha-10 isoform; arachidonic 5-lipoxygenase delta-10-13 isoform; arachidonic 5-lipoxygenase delta-13 isoform; arachidonic 5-lipoxygenase delta-p10 isoform; arachidonic acid 5-lipoxygenase; leukotriene A4 synthase; LOX-5; lox5; Polyunsaturated fatty acid 5-lipoxygenase; RP11-67C2.3

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Gene Aliases: 5-LO; 5-LOX; 5LO; 5LPG; 5LX; AI850497; ALOX5; F730011J02; LOG5

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UniProt ID: (Human) P09917, (Mouse) P48999

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Entrez Gene ID: (Human) 240, (Mouse) 11689

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arachidonate 5-lipoxygenase activity iron ion binding protein binding oxidoreductase activity oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen metal ion binding dioxygenase activity oxygenase reverse transcriptase

Process(es)

acute inflammatory response leukotriene production involved in inflammatory response leukotriene metabolic process response to nutrient sensory perception of pain arachidonic acid metabolic process leukotriene biosynthetic process lipoxygenase pathway positive regulation of vasoconstriction response to hyperoxia oxidation-reduction process lipoxin metabolic process inflammatory response

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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