Active Motif

5-Methylcytosine (5-mC) Monoclonal Antibody (33D3)

5-Methylcytosine (5-mC) Antibody in Immunohistochemistry (IHC)

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5-Methylcytosine (5-mC) Antibody (39649) in IHC

5-Methylcytosine (5-mc) antibody (pAb) tested by Immunohistochemistry Punctate nuclear staining pattern is detected in Formalin-fixed, paraffin-embedded tissue sections from human substantia nigra (midbrain). Top Panel: 5-mC antibody at 1:1000 dilution. Bottom Panel: No primary antibody (2nd step antibody alone).

5-Methylcytosine (5-mC) Antibody in Methylated DNA Immunoprecipitation (MeDIP)

Product Image Gallery

Product Details

39649

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

1:1,000

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 3 publications 3 publications

ELISA (ELISA)

1:10,000

ChIP-sequencing (ChIP-Seq)

View 2 publications 2 publications

Dot blot (DB)

View 2 publications 2 publications

Miscellaneous PubMed (Misc)

View 4 publications 4 publications

Methylated DNA Immunoprecipitation (MeDIP)

1 µg

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse, Non-human primate

Host/Isotype

Mouse / IgG

Class

Monoclonal

Type

Antibody

Clone

33D3

Immunogen

Clone 33D3 recognizes the modified base 5-methylcytidine found in plant and vertebrate DNA.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

10mM phosphate, pH 7.5, with 0.15M NaCl

Contains

0.01% thimerosal

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2687950

Target Information

Methylation of DNA cytosine bases plays important roles in the regulation of gene transcription. It has been estimated that 60 to 90% of the cytosines in CpG dinucleotides are methylated. An increase in methyl-CpG correlates with transcriptional silencing for the whole chromosome especially in developmentally regulated genes. Methylation is believed to lead to transcriptional silencing by multiple mechanisms including alteration of transcription factor binding to promoters and alteration in chromatin structure.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Integrin α6 signaling induces STAT3-TET3-mediated hydroxymethylation of genes critical for maintenance of glioma stem cells.

Oncogene

Herrmann A,Lahtz C,Song J,Aftabizadeh M,Cherryholmes GA,Xin H,Adamus T,Lee H,Grunert D,Armstrong B,Chu P,Brown C,Lim M,Forman S,Yu H

Research topic: Cancer, Glioma, stem cells

Sun Mar 01 00:00:00 EST 2020

Small-Molecule Targeting of Oncogenic FTO Demethylase in Acute Myeloid Leukemia.

Cancer cell

Huang Y,Su R,Sheng Y,Dong L,Dong Z,Xu H,Ni T,Zhang ZS,Zhang T,Li C,Han L,Zhu Z,Lian F,Wei J,Deng Q,Wang Y,Wunderlich M,Gao Z,Pan G,Zhong D,Zhou H,Zhang N,Gan J,Jiang H,Mulloy JC,Qian Z,Chen J,Yang CG

Research topic: acute myeloid leukemia (AML)

Mon Apr 15 00:00:00 EDT 2019

Global DNA Methylation and Histone Posttranslational Modifications in Human and Nonhuman Primate Brain in Association with Prenatal Alcohol Exposure.

Alcoholism, clinical and experimental research

Jarmasz JS,Stirton H,Basalah D,Davie JR,Clarren SK,Astley SJ,Del Bigio MR

Research topic: Neurobiology, Alcoholism

Sat Jun 01 00:00:00 EDT 2019

Global DNA Methylation and Histone Posttranslational Modifications in Human and Nonhuman Primate Brain in Association with Prenatal Alcohol Exposure.

Alcoholism, clinical and experimental research

Jarmasz JS,Stirton H,Basalah D,Davie JR,Clarren SK,Astley SJ,Del Bigio MR

Research topic: Neurobiology, Alcoholism

Sat Jun 01 00:00:00 EDT 2019

Analysis of diet-induced differential methylation, expression, and interactions of lncRNA and protein-coding genes in mouse liver.

Scientific reports

Silva JP,van Booven D

Research topic: Liver

Wed Aug 01 00:00:00 EDT 2018

PTP1B controls non-mitochondrial oxygen consumption by regulating RNF213 to promote tumour survival during hypoxia.

Nature cell biology

Banh RS,Iorio C,Marcotte R,Xu Y,Cojocari D,Rahman AA,Pawling J,Zhang W,Sinha A,Rose CM,Isasa M,Zhang S,Wu R,Virtanen C,Hitomi T,Habu T,Sidhu SS,Koizumi A,Wilkins SE,Kislinger T,Gygi SP,Schofield CJ,Dennis JW,Wouters BG,Neel BG

Research topic: Hypoxia

Fri Jul 01 00:00:00 EDT 2016

DNA Hypomethylation and Histone Variant macroH2A1 Synergistically Attenuate Chemotherapy-Induced Senescence to Promote Hepatocellular Carcinoma Progression.

Cancer research

Borghesan M,Fusilli C,Rappa F,Panebianco C,Rizzo G,Oben JA,Mazzoccoli G,Faulkes C,Pata I,Agodi A,Rezaee F,Minogue S,Warren A,Peterson A,Sedivy JM,Douet J,Buschbeck M,Cappello F,Mazza T,Pazienza V,Vinciguerra M

Research topic: Liver Cancer

Mon Feb 01 00:00:00 EST 2016

Tet1 and Tet2 Protect DNA Methylation Canyons against Hypermethylation.

Molecular and cellular biology

Wiehle L,Raddatz G,Musch T,Dawlaty MM,Jaenisch R,Lyko F,Breiling A

Research topic: DNA Methylation

Mon Feb 01 00:00:00 EST 2016

Iodine-125 irradiation inhibits invasion of gastric cancer cells by reactivating microRNA-181c expression.

Oncology letters

Yang Y,Ma ZH,Li XG,Zhang WF,Wan J,Du LJ,Li GJ,Yang GK,Lu P

Research topic: Gastric Cancer

Sat Oct 01 00:00:00 EDT 2016

Paternal under-nutrition programs metabolic syndrome in offspring which can be reversed by antioxidant/vitamin food fortification in fathers.

Scientific reports

McPherson NO,Fullston T,Kang WX,Sandeman LY,Corbett MA,Owens JA,Lane M

Research topic: Metabolism

Fri Jun 03 00:00:00 EDT 2016

TET1 Depletion Induces Aberrant CpG Methylation in Colorectal Cancer Cells.

PloS one

Kai M,Niinuma T,Kitajima H,Yamamoto E,Harada T,Aoki H,Maruyama R,Toyota M,Sasaki Y,Sugai T,Tokino T,Nakase H,Suzuki H

Research topic: Colorectal

Wed Jul 05 00:00:00 EDT 2017

Hemi-methylated DNA regulates DNA methylation inheritance through allosteric activation of H3 ubiquitylation by UHRF1.

eLife

Harrison JS,Cornett EM,Goldfarb D,DaRosa PA,Li ZM,Yan F,Dickson BM,Guo AH,Cantu DV,Kaustov L,Brown PJ,Arrowsmith CH,Erie DA,Major MB,Klevit RE,Krajewski K,Kuhlman B,Strahl BD,Rothbart SB

Research topic: Epigenetic Inheritance

Tue Sep 06 00:00:00 EDT 2016

AF9 promotes hESC neural differentiation through recruiting TET2 to neurodevelopmental gene loci for methylcytosine hydroxylation.

Cell discovery

Qiao Y,Wang X,Wang R,Li Y,Yu F,Yang X,Song L,Xu G,Chin YE,Jing N

Research topic: Brain & Nerves

Wed Jul 27 00:00:00 EDT 2016

Genome-Wide Mapping of 5mC and 5hmC Identified Differentially Modified Genomic Regions in Late-Onset Severe Preeclampsia: A Pilot Study.

PloS one

Zhu L,Lv R,Kong L,Cheng H,Lan F,Li X

Research topic: Preeclampsia

Fri May 06 00:00:00 EDT 2016

Alteration of genic 5-hydroxymethylcytosine patterning in olfactory neurons correlates with changes in gene expression and cell identity.

Proceedings of the National Academy of Sciences of the United States of America

Colquitt BM,Allen WE,Barnea G,Lomvardas S

Research topic: Neurobiology

Tue Sep 03 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: 5-mC; 5-meC; 5-Methyl-cytosine; 5mc; C5H7NO3

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Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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5-Methylcytosine (5-mC) Monoclonal Antibody (33D3)

5-Methylcytosine (5-mC) Antibody (39649) in IHC

Product Details

39649

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

ChIP-sequencing (ChIP-Seq)

Dot blot (DB)

Miscellaneous PubMed (Misc)

Methylated DNA Immunoprecipitation (MeDIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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