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5-Methylcytosine Antibody (MA5-38432) in ICC/IF
Immunocytochemical analysis of 5-Methylcytosine in HeLa cells using a 5-Methylcytosine monoclonal antibody (Product # MA5-38432). The cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 1% BSA. The cells were immunofluorescently labelled with the 5-mC antibody (middle) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa594. The le... View More
Product Details
MA5-38432
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chemical
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
33D3
Immunogen
5-Methylcytosine nucleotide
Conjugate
Form
Liquid
Concentration
1.1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.05% sodium azide
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2898346
Target Information
Methylation of DNA cytosine bases plays important roles in the regulation of gene transcription. It has been estimated that 60 to 90% of the cytosines in CpG dinucleotides are methylated. An increase in methyl-CpG correlates with transcriptional silencing for the whole chromosome especially in developmentally regulated genes. Methylation is believed to lead to transcriptional silencing by multiple mechanisms including alteration of transcription factor binding to promoters and alteration in chromatin structure.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 5-mC; 5-meC; 5-Methyl-cytosine; 5mc; C5H7NO3
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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