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Invitrogen
5-hydroxymethylcytosine Polyclonal Antibody
Advanced Verification
This Antibody was verified by Peptide array to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 4
5-hydroxymethylcytosine Antibody (PA5-40097) in IP
hMeDIP (hydroxymethylated DNA IP) was performed using a 5-hmC/5-hydroxymethylcytosine polyclonal antibody (Product # PA5-40097). DNA from mouse ES cells was prepared with the GenDNA module of the hMeDIP kit and sonicated with our Bioruptor® (UCD-200/300 series) to obtain DNA fragments of 300-500 bp. One µg of sheared DNA was spiked with the unmethylated (C) methylated (mC), and hydroxymethylated (hmC) contr... View More
Product Details
PA5-40097
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Human KLH-conjugated 5-hydroxymethylcytosine.
Conjugate
Form
Liquid
Concentration
3.5 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.4
Contains
0.05% ProClin 300, 0.05% sodium azide
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2610671
Target Information
5-hydroxymethylcytosine (5-hmC) has been recently discovered in mammalian DNA. 5-hmC results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of oxygenases. Initially, the 5-hmC base had been identified in Purkinje neurons, in granule cells and embryonic stem cells where it is present at high levels (up to 0,6% of total nucleotides in Purkinje cells). A recent report indicates that 5-hmC is also abundant in brain tissue, especially in areas that are associated with higher cognitive functions.Early evidence suggests that 5-hmC may represent an intermediate in a new pathway which demethylates DNA, converting 5-mC to cytosine. Although its precise role has still to be shown, recent results indicate, however, that 5-hmC may play important roles distinct from 5-mC. This may open up entirely new perspectives in epigenetic studies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 5-hmc; 5-HydroxymethylCytidine; 5hmc; C5H7N3O2
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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