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Invitrogen
5-hydroxymethylcytosine Monoclonal Antibody (DTA1002)
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5-hydroxymethylcytosine Antibody (MA5-38433) in MeDIP
Methylated DNA immunoprecipitation analysis of 5-hydroxymethylcytosine in 1 µg fragmented human genomic DNA using 0.2 µg of a 5-Methylcytosine monoclonal antibody (Product # MA5-38433). A mouse IgG isotype antibody was used as a negative control. DNA fragments of 300-500 bp from 1 μg of human Hela cells were spiked with non-methylated, methylated, and hydroxymethylated PCR fragments. The immunoprecipitated ... View More
Product Details
MA5-38433
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chemical
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
DTA1002
Immunogen
5-Hydroxymethylcytosine nucleotide
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS
Contains
0.05% sodium azide
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2898347
Target Information
5-hydroxymethylcytosine (5-hmC) has been recently discovered in mammalian DNA. 5-hmC results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of oxygenases. Initially, the 5-hmC base had been identified in Purkinje neurons, in granule cells and embryonic stem cells where it is present at high levels (up to 0,6% of total nucleotides in Purkinje cells). A recent report indicates that 5-hmC is also abundant in brain tissue, especially in areas that are associated with higher cognitive functions.Early evidence suggests that 5-hmC may represent an intermediate in a new pathway which demethylates DNA, converting 5-mC to cytosine. Although its precise role has still to be shown, recent results indicate, however, that 5-hmC may play important roles distinct from 5-mC. This may open up entirely new perspectives in epigenetic studies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 5-hmc; 5-HydroxymethylCytidine; 5hmc; C5H7N3O2
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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