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Active Motif
5-methylcytosine Recombinant Mouse Monoclonal Antibody, AbFlex®
FIGURE: 1 / 1
5-methylcytosine Antibody (91187) in DB
Dot blot of AbFlex® 5-methylcytosine antibody (rAb). DNA standards from Cat# 55008 (5, 1 and 0.2 ng as indicated) were spotted onto positively charged nylon membrane and blotted with AbFlex 5-methylcytosine recombinant antibody at a 1 µg/mL. Lane 1: Unmethylated DNA. Lane 2: DNA containing 5-methylcytosine. Lane 3: DNA containing 5-hydroxymethylcytosine.
Product Details
91187
Product Specifications
Species Reactivity
Chemical
Host/Isotype
Mouse
/ IgG2a
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Immunogen
This antibody was raised against 5-methylcytosine conjugated to kLH.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
140mM HEPES/32mM sodium acetate, pH 7.5, with 30% glycerol, 70mM NaCl
Contains
0.035% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2793794
Target Information
Methylation of DNA cytosine bases plays important roles in the regulation of gene transcription. It has been estimated that 60 to 90% of the cytosines in CpG dinucleotides are methylated. An increase in methyl-CpG correlates with transcriptional silencing for the whole chromosome especially in developmentally regulated genes. Methylation is believed to lead to transcriptional silencing by multiple mechanisms including alteration of transcription factor binding to promoters and alteration in chromatin structure.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 5-mC; 5-meC; 5-Methyl-cytosine; 5mc; C5H7NO3
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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