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Invitrogen
CD169 (Siglec-1) Monoclonal Antibody (SER-4), eFluor™ 660, eBioscience™
FIGURE: 1 / 3
CD169 (Siglec-1) Antibody (50-5755-82) in Flow
Staining of C57Bl/6 bone marrow cells with Anti-Mouse CD11b FITC (Product # 11-0112-41) and 0.125 µg of Rat IgG2a K Isotype Control eFluor® 660 (Product # 50-4321-82) (left) or 0.125 µg of Anti-Mouse CD169 eFluor® 660 (right). Total cells were used for analysis.
Product Details
50-5755-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rat
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
SER-4
Conjugate
Excitation/Emission Max
651/668 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2574241
Product Specific Information
Description: The SER-4 monoclonal antibody reacts with mouse CD169, which is also known as Sialoadhesin and Siglec-1. CD169 is a 185-kDa member of the Siglec family of adhesion and endocytic receptors that recognize sialylated glycans. This receptor is expressed on a subset of macrophages that are found with high frequency in the spleen, liver, lymph node, and bone marrow. CD169 can also be detected on monocytes in the bone marrow and periphery. This receptor plays a role in cell-cell adhesion, as well as internalization of sialylated virus and bacteria. This endocytic pathway allows CD169+ macrophages to present lipid antigens to iNKT cells, thereby leading to their activation. Studies have also demonstrated that direct interaction of CD169 with regulatory T cells inhibits their proliferation.
The SER-4 antibody has been reported to have antagonist activity.
Applications Reported: This SER-4 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This SER-4 antibody has been tested by flow cytometric anaylsis of mouse bone marrow cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.
Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
The siglecs are a family of membrane bound lectins (of the immunoglobulin superfamily) that bind sialic acid and mediate cell-cell interactions. Family members include sialoadhesin, CD22 and CD33. Sialoadhesin is a macrophage-specific cell adhesion molecule that is expressed on activated macrophages in chronic inflammation and in tumours.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD169; SER; Sheep erythrocyte receptor; Sialic acid-binding Ig-like lectin 1; Sialoadhesin; Siglec-1
Gene Aliases: Cd169; Sa; Siglec-1; Siglec1; Sn
UniProt ID: (Mouse) Q62230
Entrez Gene ID: (Mouse) 20612
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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